Skip to main content

Comparison of desferrioxamine and NODAGA for the gallium-68 labeling of exendin-4



Radiolabeled exendin-4 (Ex4) derivatives are used to target the glucagon-like peptide-1 receptor (GLP-1R) for the clinical diagnosis of insulinomas, a rare type of neuroendocrine tumor. Gallium-68 is an ideal diagnostic nuclide for this application and a study evaluating an exendin-4-NODAGA conjugate is currently underway. However, in complexion with the chelator DFO, its in vivo stability has been a matter of dispute. The aim of this work was to directly compare [68Ga]Ga-Ex4NOD with [68Ga]Ga-Ex4DFO in vitro and in vivo.


In our approach, we directly compared N′-[5-(acetyl-hydroxy-amino)pentyl]-N-[5-[3-(5-aminopentyl-hydroxy-carbamoyl)propanoylamino]pentyl]-N-hydroxy-butane diamide (desferriox-amine B, DFO) and 2-(4,7-bis (carboxymethyl)-1,4,7-triazonan-1-yl) pentanedioic acid (NODAGA) conjugated to exendin-4 in vitro and in vivo. We radiolabeled the peptides with gallium-68, followed by HPLC quality control. In vitro characterization was performed in CHL cells overexpressing the GLP-1R and in vivo studies were conducted with CD1 nu/nu mice carrying tumors derived from these cells.


We found that both peptides could be radiolabeled with a molar activity of about 9.33 MBq/nmol without further purification. They internalized equally well into GLP-1R-expressing cells and their IC50 was similar with 15.6 ± 7.8 nM and 18.4 ± 3.0 nM for [natGa]Ga-Ex4NOD and [natGa]Ga-Ex4DFO, respectively. In vivo, [68Ga]Ga-Ex4NOD accumulated more in all tissue, while [68Ga]Ga-Ex4DFO exhibited a more favorable target-to-kidney ratio.

Conclusion and relevance

DFO is a suitable chelator for the radiolabeling of exendin-4 derivatives with gallium-68 for in vitro and preclinical in vivo studies. DFO performed better in vivo due to its significantly lower kidney accumulation (p < 0.0001). It was also found to be stable in vivo in mice, contrary to earlier reports. Based on our results, the DFO chelating system in combination with exendin-4 would be an interesting option for clinical imaging of insulinomas.


Insulinomas, a form of neuroendocrine tumors originating from pancreatic β-cells and are usually benign. However, in a minority of cases malignant tumors are found at time of diagnosis (Hirshberg et al. 2000; Service et al. 1991). Insulinomas belong to the functional type of pancreatic neuroendocrine tumors (PNET) as they secrete insulin (Burns and Edil 2012). Due to this autonomous insulin secretion, hypoglycemia is a major concern in patients and the complete surgical removal of such tumors is the favored treatment (Burns and Edil 2012).

Insulinomas generally express highly and specifically the glucagon-like peptide-1 receptor (GLP-1R) as they are of β-cell origin (Reubi and Waser 2003). GLP-1R is a G-protein coupled receptor of the class B receptor family (Zhang et al. 2017). Its endogenous peptide ligand, GLP-1, has a very short biological half-life of about 2 min. Dipeptidyl peptidase-4 (DPP4) is responsible for the cleavage of the peptide (Baggio and Drucker 2007). GLP-1R can also be targeted by several more stable GLP-1 derivatives, such as liraglutide and semaglutide, and analogues, such as lixisenatide and exendin-4. All of them are clinically used in non-radiolabeled form for the management of type 2 diabetes (Aroda 2018).

Currently, exendin-4 coupled to the chelator 2-(4,7-bis (carboxymethyl)-1,4,7-triazonan-1-yl) pentanedioic acid (NODAGA) is in clinical evaluation of adult hyperinsulinemic hypoglycemia (EudraCT Number: 2014–003167-38) (68Ga-NODAGA-exendin-4 PET/CT for Diagnostic Imaging in AHH n.d.). Furthermore, NODAGA is used in preclinical imaging studies with gallium-68 and it is also frequently investigated with copper-64, another interesting PET radionuclide with a half-life of 12 h, which has been used previously with exendins and other peptidic tracers (Nedrow et al. 2014; Mikkola et al. 2014). N′-[5-(acetyl-hydroxy-amino)pentyl]-N-[5-[3-(5-aminopentyl-hydroxy-carbamoyl)-propanoylamino]pentyl]-N-hydroxy-butane diamide (desferrioxamine B, DFO) DFO has mainly been investigated as a chelator for zirconium-89 labeling of antibodies (immunoPET) (Verel et al. 2003; Mayer et al. 2017; Brandt et al. 2018).

We previously described an exendin-4 tracer conjugated to the chelator DFO for use with either the short-lived gallium-68 or the longer-lived PET nuclide zirconium-89, for the imaging at later timepoints or in combination with radioguided surgery (Bauman et al. 2015). We could show that the tumor uptake with [68Ga]Ga-Ex4DFO was higher than with the clinically tested SPECT tracer [111In]In-DTPA-exendin-4.

In the present study, we expanded on previous encouraging findings made with DFO-exendin-4 and investigated how NODAGA-exendin-4 compared in vitro and in vivo when used with the standard PET nuclide gallium-68. A gallium-68 labeled NODAGA-conjugated exendin-4 derivative is currently investigated in clinics for the diagnosis of adult hyperinsulinemic hypoglycemia and insulinoma (68Ga-NODAGA-exendin-4 PET/CT for Diagnostic Imaging in AHH n.d.). A prevailing issue with radiometal labeled exendin-4 derivatives is the persistent kidney accumulation (Simonsen et al. 2006; Eriksson et al. 2016). Insulinomas are frequently located within the pancreas and therefore in close proximity to the kidneys. SPECT imaging has been shown not to achieve sufficient resolution to detect them. Instead, PET is the favored imaging modality with its higher resolution, as shown very clearly by Antwi and colleagues (Antwi et al. 2015). Therefore, we also aimed to evaluate Ex4DFO and Ex4NOD with respect to their accumulation in the kidneys as chelators are known to have a substantial effect on the biodistribution of a tracer (Price and Orvig 2014).


Peptides and labeling

Exendin-4 was conjugated to N′-[5-(acetyl-hydroxy-amino)pentyl]-N-[5-[3-(5-aminopentyl-hydroxy-carbamoyl)propanoylamino]pentyl]-N-hydroxy-butane diamide (desferrioxamine, DFO) to give rise to [Lys40-(Ahx-DFO)NH2]exendin-4 as described in (Bauman et al. 2015), in short Ex4DFO. In the second derivative, exendin-4 was modified at position 14 where methionine (Met) was replaced with Norleucine (Nle) to improve oxidative stability during labeling (Velikyan et al. 2017). Then, it was conjugated to 2-(4,7-bis (carboxymethyl)-1,4,7-triazonan-1-yl) pentanedioic acid (NODAGA) to give rise to [Nle14-Lys40-(NODAGA)NH2]exendin-4, in short Ex4NOD. It was synthesized commercially (piChem, Graz, Austria). The schematic structures of the peptides are shown in Additional file 1: Figure S1.

For the radiolabeling, gallium-68 was freshly eluted with 0.1 M HCl (Rotem Industries, Israel) from a IGG100 68Ge/68Ga Generator (Eckert & Ziegler, Berlin, Germany) through a plastic needle from a generator into a glass vial, which was prewashed with metal-free 0.1 mM HCl and flushed with metal free analytical grade water (Sigma-Aldrich, Buchs, Switzerland). Typically, an activity of about 950 to 990 MBq in 6.5 to 7.0 mL was obtained (activity concentration of about 140 MBq/mL). Briefly, the labeling was performed as follows. Six μL of peptide (250 μM) was added to 50 μL of 1 M ammonium acetate (pH 5.5) and 2 μL of 3 M NaOH in a 1.5 mL screw-top tube. Then, 100 μL of gallium-68 (14 MBq) was added and the mixture was incubated at 95 °C for 10 min in a heating block, after which it was spun down. One μL of the labeling mixture was diluted into 150 μL of 1% ethylenediaminetetraacetic acid (EDTA) in water and analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) on a ReproSil-Pur C18-AQ column (100 × 4.6 mm, 3 μm, Dr. Maisch, Germany). The column was eluted with H2O containing 0.1% trifluoroacetic acid (TFA), with a linear gradient from 15%–55% of acetonitrile containing 0.1% TFA for 10 min, followed by a linear gradient from 55%–70% for 5 min. The peptide peaks were observed at about 9.40 min (Ex4NOD) and 9.20 min (Ex4DFO) and the yields and purity of labeled peptides were typically ≥95%. No further purification was performed.

The labeling with the stable isotope was performed as described previously (Jodal et al. 2015). Briefly, 40 μL of the respective 250 μM peptide solution were added to 2 μL of a 10 mM [natGa]GaCl3 (Sigma-Aldrich, Buchs, Switzerland) solution and mixed with 60 μL of 0.5 M ammonium acetate buffer (pH 5.5). The reaction mixture was incubated at 75 °C for 30 min. 10 μL of 0.1 mM diethylenetriaminepentaacetic acid (DTPA) was added after incubation. The success of the labeling was verified by liquid chromatography-mass spectrometry (LC/MS) on an Atlantis C18 (250 × 4.6 mm; 5 μm) column on a Waters LCT Premier mass spectrometer (Milford, USA).

For the IC50 experiments, Ex4NOD was labeled with indium-111 as follows. Three MBq of [111In]InCl3 was added to the reaction mixture containing a final concentration of 10 μM of the peptide, 5 μL of 0.5 M ammonium acetate (pH 5.5) and 0.02 M HCl (adjusting the amount of [111In]InCl3 to 25 μL). The quality control was performed as described above. [111In]InCl3 was purchased from Mallinckrodt (Cham, Switzerland).

Cell culture

Throughout the study Chinese hamster lung (CHL) cells stably transfected with human GLP-1R were used (a kind gift of Prof. Brigitte Lankat-Buttgereit). They were cultured in Dulbecco’s modified Eagle medium (DMEM) containing 4.5 g/L D-glucose, to which 10% fetal calf serum (FCS), 100 IU/mL penicillin G, 100 μg/mL streptomycin, 1.25 μg/mL fungizone®, 500 μg/mL geneticin sulfate, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids and 2 mM L-glutamine were added. The cells were maintained at 37 °C in a humidified 5% CO2 atmosphere (Jodal et al. 2014, 2015). They were harvested with trypsin/EDTA. The trypsin was removed by centrifugation at 1000 g for 5 min.

Competition binding (IC50) assay

To determine the half-maximal inhibitory concentration (IC50) of the exendin-4 derivatives, the cells were seeded in 12-well plates at 0.2 × 106 cells per well and grown over night to reach a confluency of about 90–95%. The peptide under investigation was labeled with the stable isotope as described above, the reference peptide Ex4NOD was labeled with [111In]InCl3 as described above. The cells were washed twice with cold PBS (0.1 M NaCl, 0.042 M Na2HPO4, 0.011 M NaH2PO4) and incubated for 1 h on ice with the stably labeled peptide concentrations from 10− 6 to 10− 11 M and 150 to 200 pM of radiolabeled Ex4NOD. After binding, the cells were washed twice with PBS and solubilized with 1 M NaOH. The activity was measured using a γ-counter (Packard Cobra II Auto Gamma, PerkinElmer, Switzerland). The IC50 values were determined by fitting the data with non-linear regression using the least-squares fit of GraphPad Prism (version 7.0, GraphPad Software, La Jolla, USA). Significance of the calculated IC50 values was assessed in Prism using multiple t-test with the Holm-Sidak method. All experiments were performed in triplicates.

Cell uptake assay

The cells were cultured as described for the IC50 assay and seeded into 6-well plates at a density of 0.75 × 106 cells per well. They were probed with 150 to 200 pM labeled peptide and incubated at 37 °C for different timepoints (5, 15, 30, 60 and 120 min) at which the supernatant was collected. The cells were washed twice with 600 μL of PBS and the wash was pooled with the supernatant. The surface-bound peptide was collected from the cells after 5 min incubation with glycine buffer (0.1 M NaCl, 0.05 M Glycine, pH 2.8) at room temperature, during which the peptide detached from the receptors on the cell membranes. This fraction was collected separately and the glycine treatment was repeated. Finally, the cells were lysed by the addition of 1 M NaOH. In the latter samples, the internalized fraction of the radiolabeled peptide could be found. The non-specific binding and internalization were determined by blocking the receptors with 1 μM of commercially available, non-labeled exendin-4 (Bachem, Bubendorf, Switzerland), which was added to the control cells together with the radiolabeled peptide. The activity of the samples was measured in the γ-counter. The measurements were decay corrected and all experiments were performed in triplicates. Significance at the 2-h timepoint was analyzed with a one-way ANOVA and multiple comparisons of the three tracers were performed using Tukey’s multiple comparisons test with α = 0.05.


All animal experiments were conducted with permission of the local veterinary office and in accordance to the Swiss law of animal protection.

Six-week old female CD1 nu/nu mice were inoculated bilaterally in the shoulders with 8 × 106 GLP-1R-expressing CHL cells (suspended in PBS, pH 7.4). Tumor growth was observed for 3 weeks after which the tumors weighed generally around 250 mg. The mice were randomly divided into the different study groups, four mice per group. All mice were injected with 100 kBq (8 to 10 pmol) of gallium-68-labeled peptide in 100 μl PBS via the tail vein. One group per peptide was co-injected with an excess of exendin-4 peptide (100 μg/mouse) to block specific tracer uptake. The mice were euthanized 0.5 or 1 h after injection of the tracer by CO2 inhalation. Organs (blood, heart, lungs, spleen, kidneys, pancreas, stomach, intestine, liver, muscle, bone) and tumors were collected in pre-weighted tubes. The activity was measured in the γ-counter. The values were decay corrected for evaluation. The percentage of injected activity per gram tissue (% i.A./g) was calculated for all collected tissues and analyzed in Excel (Microsoft, Redmond, USA). A two-way ANOVA was performed in GraphPad Prism, and comparisons of the two tracers was done using Tukey’s multiple comparisons test with α = 0.05. The target-to-kidney ratios were calculated in Microsoft Excel, and their averages, standard deviations, and significance were analyzed in Prism using two-way ANOVA as described above.


Labeling of the peptides

Both, Ex4NOD and Ex4DFO could be radiolabeled with gallium-68 according to the protocol described above. The radiochemical yield was typically over 95% and a molar activity of 9.33 MBq/nmol for both peptides. Representative HPLC chromatographs of the radiolabeled peptides are shown in Additional file 1: Figure S2.

The labeling with stable gallium was achieved and representative MS analyses of the [natGa]Ga-labeled peptides are shown in Additional file 1: Figures S3 and S4.

There was no difference in the labeling outcome between Ex4NOD and Ex4DFO even though the latter still carries the potentially oxidizable methionine at position 14, which was replaced by neurleucine in Ex4NOD.

Binding affinity to GLP-1R and receptor-mediated cell uptake

We tested the affinity to the human GLP-1 receptor expressed in CHL cells in an IC50 competition assay using indium-111 labeled Ex4NOD peptide as reference and Ex4NOD and Ex4DFO labeled with stable gallium isotope in increasing concentrations. The IC50 of [natIn]In-Ex4NOD was determined to be 22.4 ± 2.9 nM. We found that [natGa]Ga-Ex4DFO had a slightly lower IC50 than [natGa]Ga-Ex4NOD, with 18.4 ± 3.0 nM and 19.6 ± 3.4 nM, respectively. However, the difference was statistically not significant. In fact, the IC50 curves of both peptides largely overlapped, as shown in in Fig. 1a.

Fig. 1
figure 1

In vitro characterization of Ex4NOD and Ex4DFO (a) The IC50 curves of [natGa]Ga-Ex4NOD and [natGa]Ga-Ex4DFO, shown as the displacement of [111In]In-Ex4NOD from the GLP-1 receptor on CHL cells, correspond closely. b The GLP-1R-mediated cell binding and internalization show similar time-dependent trend in both gallium-68 labeled peptides, which again in turn is comparable to indium-111 labeled peptide. The percentage represents total cell uptake

Further, we investigated the pattern of GLP-1R mediated cell uptake in CHL cells. We observed an increase in internalization over 2 h, with a near-constant surface-binding, in particular towards later timepoints, as apparent in Fig. 1b. At 2 h, [68Ga]Ga-Ex4NOD reached 38.6 ± 7.4% internalization, whereas [68Ga]Ga-Ex4DFO was taken up to 43.6 ± 2.4% and the comparison peptide [111In]In-Ex4NOD showed 40.5 ± 4.2% internalization. The surface-binding was similar in all cases (14.5 ± 3.4%, 14.3 ± 1.2%, and 13.7 ± 1.5%, respectively).

Timecourse biodistribution in GLP-1R tumor bearing mice

In the timecourse biodistribution study performed with both [68Ga]Ga-Ex4NOD and [68Ga]Ga-Ex4DFO, we found that [68Ga]Ga-Ex4DFO accumulated generally less all organs and tissues, including the GLP-1R-positive lung, pancreas, and tumor, as presented in Table 1. However, when comparing the tissues expressing GLP-1R such as the CHL tumors and the pancreas, no significant difference between the tracers could be found, except in the lungs, where the uptake at 1 h p.i. was significantly higher in [68Ga]Ga-Ex4NOD (Fig. 2). The kidney uptake in both timepoints was significantly higher in [68Ga]Ga-Ex4NOD, except for the blocking group, where no significant difference to [68Ga]Ga-Ex4DFO could be detected. Tumor uptake at 1 h p.i. appeared different, but did not reach significance (p = 0.0938).

Table 1 Full timecourse biodistribution study of gallium-68 labeled Ex4NOD and Ex4DFO, given as percent injected activity per gram tissue (% i.A/g)
Fig. 2
figure 2

Selected organs from a timecourse biodistribution with [68Ga]Ga-Ex4NOD and [68Ga]Ga-Ex4DFO, performed in GLP-1R positive tumor-bearing CD 1 nu/nu mice (n = 4 per group). For both tracers, blocking at 1 h p.i. was achieved in all GLP-1R-expressing tissue

We then proceeded to calculate the target-to-kidney ratio for the tumor, lungs, and pancreas and discovered that [68Ga]Ga-Ex4DFO significantly outperformed [68Ga]Ga-Ex4NOD in that respect, as evident from Table 2.

Table 2 GLP-1R-positive tissue-to-kidney and tumor-to-GLP-1R-positive tissue ratios derived from above timecourse biodistribution


Methods for high-resolution diagnostic imaging of insulinomas continue to be investigated pre-clinically and clinically. Exendin-4-based tracers, such as [68Ga]Ga-DOTA-Exendin-4 (Antwi et al. 2015), have already shown potential in clinical trials to replace less specific diagnostic tools, e.g. the [111In]In-DTPA-octreotide scintigraphy for the visualization of somatostatin receptor-positive tissue (Shi et al. 1998), or more general imaging modalities such as CT or MRI (Noone et al. 2005).

Based on our previous study investigating the chelator DFO for applications with the radiometal zirconium-89 for PET imaging (Bauman et al. 2015), we were curious how DFO would compare to NODAGA, which is, next to the clinically employed DOTA and the pre-clinically used DTPA (Antwi et al. 2015; Christ et al. 2015; van der Kroon et al. 2016), another chelator frequently used to pre-clinically investigate exendin-based tracers (Mikkola et al. 2014; Jodal et al. 2014).

There are conflicting reports about the stability of the gallium-chelation by DFO (Wester et al. 1997; Smith-Jones et al. 1994) and the complex has been observed to decompose during labeling at high temperatures (Tsionou et al. 2017). NODAGA on the other hand was demonstrated to label well with gallium-68 at elevated temperatures providing in vivo stable complexes (Asti et al. 2014). However, their thermodynamic complex formation stability constant (log K) have been reported to be very similar, with 28.65 for DFO and 29.63 for NOTA (Šimeček et al. 2011; Evers et al. 1989).

We found [68Ga]Ga-Ex4DFO to be stable, both during labeling, and during in vivo studies, as apparent from the liver uptake, which is comparable for both peptides. Additionally, we did not observe any oxidation of methionine in the quality control for the gallium-68 and the stable isotope labeling.

We established radiolabeling protocols that could be applied to both peptides, Ex4NOD and Ex4DFO, with equally good yields. We labeled the peptides at molar activities of about 9.3 MBq/nmol, which is in the range of what was previously used clinically (Antwi et al. 2015; Eriksson et al. 2014).

We then tested the tracers side-by-side for key features in vitro and in vivo and obtained nearly identical in vitro results, both for receptor affinity and cellular uptake, suggesting that the choice of chelator may not have a large impact in cell assays. However, these results need to be interpreted in the context of the cell model used, as previously, when cell uptake was tested in the rat insulinoma cell line RIN-m5F, [Lys40-(Ahx-DFO-68Ga)NH2]exendin-4 outperformed the reference compound [Lys40-(Ahx-DTPA-111In)NH2]exendin-4 (Bauman et al. 2015).

In our previous study, Rin-m5F did not strongly internalize exendin-4 based radiotracers in vitro, reaching less than 6% internalization after 2 h (Bauman et al. 2015). This is in contrast to CHL cells stably expressing the GLP-1 receptor where we reported internalization of around 40% with both gallium-67 and indium-111 (Jodal et al. 2014, 2015). In vivo, however, the results obtained with either cell line compare well with each other.

In vivo, we observed an overall higher kidney uptake with [68Ga]Ga-Ex4NOD, whereas the uptake of [68Ga]Ga-Ex4DFO was significantly lower and therefore resulted in a more favorable GLP-1R-positive tissue-to-kidney ratio. The high kidney uptake remains to be of concern and a wide variety of strategies are currently being developed to address this problem, such as by using fluorination and iodination approaches to circumvent the issue of residualizing radiometals (Mikkola et al. 2016; Lappchen et al. 2017). However, once a strategy is found to sufficiently decrease kidney uptake, the availability of a suitable, a versatile chelator for the use of radiometals will become central to the field of application of exendin-4 based tracers. Patients suffering from insulinomas, but also other conditions, such as hyperplasias of the pancreatic β-cell, could profit from such developments.


In summary, we compared two gallium-68 labelled exendin-4 derivatives with DFO and NODAGA as a chelator. In vitro, both derivatives have similar cell uptake and affinity to GLP-1R. Nevertheless, overall, the DFO-functionalized peptide may be the better choice for imaging applications due to its significantly lower kidney uptake resulting in an improved target-to-kidney ratio.

To conclude, we believe this study should serve as a reference for consideration in future developments of exendin-4-based tracers for the targeting of GLP-1R, which will clearly remain of outmost importance.



Bovine serum albumin


Chinese hamster lung


N′-[5-(acetyl-hydroxy-amino)pentyl]-N-[5-[3-(5-aminopentyl-hydroxy-carbamoyl)propanoylamino]pentyl]-N-hydroxy-butane diamide


Dipeptidyl peptidase-4


Diethylene triamine pentaacetic acid


Ethylenediaminetetraacetic acid








Fetal calf serum


Glucagon-like peptide 1 receptor

IC50 :

Half-maximal inhibitory concentration


Liquid chromatography-mass spectrometry






2-(4,7-bis (carboxymethyl)-1,4,7-triazonan-1-yl) pentanedioic acid


Phosphate-buffered saline


Positron emission tomography


Pancreatic neuroendocrine tumor


Reversed-phase high performance liquid chromatography


Single-photon emission computed tomography


Trifluoroacetic acid


  • Antwi K, Fani M, Nicolas G, Rottenburger C, Heye T, Reubi JC, et al. Localization of hidden Insulinomas with 68Ga-DOTA-Exendin-4 PET/CT: a pilot study. J Nucl Med. 2015;56:1075–8.

    Article  CAS  PubMed  Google Scholar 

  • Aroda VR. A review of GLP-1 receptor agonists: evolution and advancement, through the lens of randomised controlled trials. Diabetes Obes Metab. 2018;20:22–33.

    Article  CAS  Google Scholar 

  • Asti M, Iori M, Capponi PC, Atti G, Rubagotti S, Martin R, et al. Influence of different chelators on the radiochemical properties of a 68-gallium labelled bombesin analogue. Nucl Med Biol. 2014;41:24–35.

    Article  CAS  PubMed  Google Scholar 

  • Baggio LL, Drucker DJ. Biology of incretins: GLP-1 and GIP. Gastroenterology. 2007;132:2131–57.

    Article  CAS  Google Scholar 

  • Bauman A, Valverde IE, Fischer CA, Vomstein S, Mindt TL. Development of 68Ga-and 89Zr-labeled Exendin-4 as potential radiotracers for the imaging of Insulinomas by PET. J Nucl Med. 2015;56:1569–74.

    Article  CAS  Google Scholar 

  • Brandt M, Cardinale J, Aulsebrook ML, Gasser G, Mindt TL. An overview of PET radiochemistry, part 2: Radiometals. J Nucl Med. 2018;59:1500–6.

    Article  CAS  Google Scholar 

  • Burns WR, Edil BH. Neuroendocrine pancreatic tumors: guidelines for management and update. Curr Treat Options in Oncol. 2012;13:24–34.

    Article  Google Scholar 

  • Christ E, Wild D, Antwi K, Waser B, Fani M, Schwanda S, et al. Preoperative localization of adult nesidioblastosis using 68Ga-DOTA-exendin-4-PET/CT. Endocrine. 2015;50:821–3.

    Article  CAS  PubMed  Google Scholar 

  • [Internet]. Bethesda (MD): National Library of Medicine (US). Identifier NCT03189953, 68Ga-NODAGA-exendin-4 PET/CT for Diagnostic Imaging in AHH (GLP-1-AHH); June 16, 2017 [cited February 15, 2019]. Available from:

  • Eriksson O, Laughlin M, Brom M, Nuutila P, Roden M, Hwa A, et al. In vivo imaging of beta cells with radiotracers: state of the art, prospects and recommendations for development and use. 2016;59:1340–1349. doi:

  • Eriksson O, Velikyan I, Selvaraju RK, Kandeel F, Johansson L, Antoni G, et al. Detection of metastatic Insulinoma by positron emission tomography with [68Ga]Exendin-4—a case report. J Clin Endocrinol Metab. 2014;99:1519–24.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Evers A, Hancock RD, Martell AE, Motekaitis RJ. Metal ion recognition in ligands with negatively charged oxygen donor groups. Complexation of iron (III), gallium (III), indium (III), aluminum (III), and other highly charged metal ions. Inorg Chem. 1989;28:2189–95.

    Article  CAS  Google Scholar 

  • Hirshberg B, Livi A, Bartlett DL, Libutti SK, Alexander HR, Doppman JL, et al. Forty-eight-hour fast: the diagnostic test for insulinoma. J Clin Endocrinol Metab. 2000;85:3222–6.

    Article  CAS  PubMed  Google Scholar 

  • Jodal A, Lankat-Buttgereit B, Brom M, Schibli R, Behe M. A comparison of three 67/68Ga-labelled exendin-4 derivatives for beta-cell imaging on the GLP-1 receptor: the influence of the conjugation site of NODAGA as chelator. EJNMMI Res. 2014;4:31.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jodal A, Pape F, Becker-Pauly C, Maas O, Schibli R, Behe M. Evaluation of 111In-labelled exendin-4 derivatives containing different meprin beta-specific cleavable linkers. PLoS One. 2015;10:e0123443.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lappchen T, Tonnesmann R, Eersels J, Meyer PT, Maecke HR, Rylova SN. Radioiodinated Exendin-4 is superior to the Radiometal-labelled glucagon-like Peptide-1 receptor probes overcoming their high kidney uptake. PLoS One. 2017;12:e0170435.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mayer AT, Natarajan A, Gordon SR, Maute RL, McCracken MN, Ring AM, et al. Practical immuno-PET radiotracer design considerations for human immune checkpoint imaging. J Nucl Med. 2017;58:538.

    Article  CAS  Google Scholar 

  • Mikkola K, Yim CB, Fagerholm V, Ishizu T, Elomaa VV, Rajander J, et al. 64Cu- and 68Ga-labelled [Nle (14),Lys (40)(Ahx-NODAGA)NH2]-exendin-4 for pancreatic beta cell imaging in rats. Mol Imaging Biol. 2014;16:255–63.

    Article  PubMed  Google Scholar 

  • Mikkola K, Yim CB, Lehtiniemi P, Kauhanen S, Tarkia M, Tolvanen T, et al. Low kidney uptake of GLP-1R-targeting, beta cell-specific PET tracer, 18F-labeled [Nle14,Lys40]exendin-4 analog, shows promise for clinical imaging. EJNMMI Res. 2016;6:91.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nedrow JR, White AG, Modi J, Nguyen K, Chang AJ, Anderson CJ. Positron emission tomographic imaging of copper 64- and gallium 68-labeled chelator conjugates of the somatostatin agonist tyr3-octreotate. Mol Imaging. 2014;13.

  • Noone TC, Hosey J, Firat Z, Semelka RC. Imaging and localization of islet-cell tumours of the pancreas on CT and MRI. Best Pract Res Clin Endocrinol Metab. 2005;19:195–211.

    Article  PubMed  Google Scholar 

  • Price EW, Orvig C. Matching chelators to radiometals for radiopharmaceuticals. Chem Soc Rev. 2014;43:260–90.

    Article  CAS  PubMed  Google Scholar 

  • Reubi JC, Waser B. Concomitant expression of several peptide receptors in neuroendocrine tumours: molecular basis for in vivo multireceptor tumour targeting. Eur J Nucl Med Mol I. 2003;30:781–93.

    Article  CAS  Google Scholar 

  • Service FJ, McMahon MM, O'Brien PC, Ballard DJ. Functioning insulinoma--incidence, recurrence, and long-term survival of patients: a 60-year study. Mayo Clin Proc. 1991;66:711–9.

    Article  CAS  Google Scholar 

  • Shi W, Johnston CF, Buchanan KD, Ferguson WR, Laird JD, Crothers JG, et al. Localization of neuroendocrine tumours with [111In] DTPA-octreotide scintigraphy (Octreoscan): a comparative study with CT and MR imaging. QJM. 1998;91:295–301.

    Article  CAS  Google Scholar 

  • Šimeček J, Schulz M, Notni J, Plutnar J, Kubíček VC, Havlíčková J, et al. Complexation of metal ions with TRAP (1, 4, 7-triazacyclononane phosphinic acid) ligands and 1, 4, 7-triazacyclononane-1, 4, 7-triacetic acid: phosphinate-containing ligands as unique chelators for trivalent gallium. Inorg Chem. 2011;51:577–90.

    Article  Google Scholar 

  • Simonsen L, Holst JJ, Deacon C. Exendin-4, but not glucagon-like peptide-1, is cleared exclusively by glomerular filtration in anaesthetised pigs. Diabetologia. 2006;49:706–12.

    Article  CAS  Google Scholar 

  • Smith-Jones PM, Stolz B, Bruns C, Albert R, Reist HW, Fridrich R, et al. Gallium-67/gallium-68-[DFO]-octreotide - a potential radiopharmaceutical for PET imaging of somatostatin receptor-positive tumors: synthesis and radiolabeling in vitro and preliminary in vivo studies. J Nucl Med. 1994;35:317–25.

    CAS  PubMed  Google Scholar 

  • Tsionou MI, Knapp CE, Foley CA, Munteanu CR, Cakebread A, Imberti C, et al. Comparison of macrocyclic and acyclic chelators for gallium-68 radiolabelling. RSC Adv. 2017;7:49586–99.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • van der Kroon I, Joosten L, Nock BA, Maina T, Boerman OC, Brom M, et al. Improved quantification of the Beta cell mass after pancreas visualization with 99mTc-demobesin-4 and Beta cell imaging with 111In-exendin-3 in rodents. Mol Pharm. 2016;13:3478–83.

    Article  CAS  PubMed  Google Scholar 

  • Velikyan I, Rosenstrom U, Eriksson O. Fully automated GMP production of [(68) Ga]Ga-DO3A-VS-Cys (40)-Exendin-4 for clinical use. Am J Nucl Med Mol Imaging. 2017;7:111–25.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Verel I, Visser GWM, Boellaard R, Stigter-van Walsum M, Snow GB, van Dongen GAMS. 89Zr Immuno-PET: comprehensive procedures for the production of 89Zr-labeled monoclonal antibodies. J Nucl Med. 2003;44:1271–81.

    CAS  PubMed  Google Scholar 

  • Wester H-J, Brockmann J, Rösch F, Wutz W, Herzog H, Smith-Jones P, et al. PET-pharmacokinetics of 18F-octreotide: a comparison with 67Ga-DFO and 86Y-DTPA-octreotide. Nucl Med Biol. 1997;24:275–86.

    Article  CAS  Google Scholar 

  • Zhang Y, Sun B, Feng D, Hu H, Chu M, Qu Q, et al. Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein. Nature. 2017;546:248–53.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references


The authors would like to thank Alain Blanc for the assistance in establishing the labeling procedure, Stefan Landolt and Marc Tautschnig for delivering gallium-68, and Saskia Müller for her help in conducting the animal studies.


This study received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 602812.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Additional data referenced in the manuscript can be found in the supporting information.

Author information

Authors and Affiliations



The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. SK has planned, conducted, and analyzed the experiments and written the manuscript, TLM and MB have designed the study, planned and analyzed the experiments and critically read the manuscript.

Corresponding author

Correspondence to Martin Behe.

Ethics declarations

Ethics approval

Animal experimentation.

Ethical approval for animal experimentation has been granted by the ethics committee for animal experimentation of the Cantons of Aargau and Basel. All experiments have been conducted in accordance to the Swiss law of animal protection.

Consent for publication

Not applicable.

Competing interests

Patent disclosure: Martin Béhé declares that they are inventors of the patent: Invention affecting GLP-1 and exendin. Philipps-Universität Marburg, June 17th 2009. All other authors declare that they have no conflict of interest.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Additional file

Additional file 1:

Figure S1. Schematic representation of the peptides investigated in the study. Figure S2. RP-HPLC chromatograph of [68Ga]Ga-Ex4NOD (top) and [68Ga]Ga-Ex4DFO (bottom). Figure S3. Mass spectrometric analysis of [natGa]Ga-Ex4NOD. Figure S4. Mass spectrometric analysis of [natGa]Ga-Ex4DFO. (DOCX 812 kb)

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaeppeli, S.A.M., Schibli, R., Mindt, T.L. et al. Comparison of desferrioxamine and NODAGA for the gallium-68 labeling of exendin-4. EJNMMI radiopharm. chem. 4, 9 (2019).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: