Skip to main content

Advertisement

[11C]Carbon monoxide: advances in production and application to PET radiotracer development over the past 15 years

Article metrics

  • 556 Accesses

Abstract

[11C]Carbon monoxide is an appealing synthon for introducing carbon-11 at a carbonyl position (C=O) in a wide variety of chemotypes (e.g., amides, ketones, acids, esters, and ureas). The prevalence of the carbonyl group in drug molecules and the present-day broad versatility of carbonylation reactions have led to an upsurge in the production of this synthon and in its application to PET radiotracer development. This review focuses on the major advances of the past 15 years.

Background

Carbon-11 is an unstable isotope that has a short half-life of 20.4 min. This radioisotope decays to stable boron-11 predominantly by positron emission (99.79%) and to a very low extent by electron capture (0.21%). Replacement of a carbon atom in an organic compound (e.g., drug or biomolecule) with carbon-11 does not modify its biological or physicochemical properties to any appreciable extent (Pike 1997; Scott 2009). These are attractive features for using carbon-11 to develop radiotracers for application with the highly sensitive molecular imaging technique of positron emission tomography (PET).

The short half-life of carbon-11 allows more than one PET experiment in a single day and in the same subject (Antoni 2015), enhancing the speed of data collection and the throughput of subjects. The possibility to perform more than one study in 1 day in the same subject facilitates test-retest studies and comparison of baseline PET data with PET data after a pharmacological intervention (e.g., blocking studies). Consequently, carbon-11 has a uniquely valuable role in expanding the biomedical and clinical applications of PET. However, radiolabeling with carbon-11 requires quick and efficient methods to maximize radiotracer yields (Långström et al. 2007, Dahl et al. 2017a).

Carbon-11 can be produced in high activity, commonly up to about 100 GBq, and with a high molar activity (ratio of radioactivity to mass; Am) often in a range of 40 to 750 GBq/μmol at the end of synthesis (Gómez-Vallejo et al. 2012) but also up to 9.7 TBq/μmol (Kihlberg et al. 2002; Noguchi and Suzuki 2003; Zhang and Suzuki 2005). By comparison, a single dose of a PET radiotracer for an experiment in a human subject is about 400 MBq. In our experience, a radiotracer Am that is greater than 40 GBq/μmol is acceptable for most PET experiments (Dahl et al. 2018). Arbitrarily, the following qualitative description scale for Am (GBq/μmol) values has been used in this review: 5–50 GBq/μmol: low; 50–100 GBq/μmol: moderate; 100–200 GBq/μmol: good; 200–400 GBq/μmol: high; 400 GBq/μmol or above: very high.

Carbon-11 is generally produced with a cyclotron by proton bombardment of nitrogen gas according to the 14N(p,α)11C nuclear reaction. Bombardment in the presence of oxygen (0.5–1%) or hydrogen (5–10%) gives [11C]carbon dioxide or [11C]methane, respectively. Oxygen or hydrogen can be present after [11C]carbon dioxide and [11C]methane production, respectively. Oxygen and potential radioactive impurities (e.g., oxygen-15) can be removed by concentrating the cyclotron-produced [11C]carbon dioxide in a cryogenic trap (at liquid nitrogen or argon temperature) or over activated molecular sieves, placed after the output of the cyclotron target chamber. Hydrogen can be eliminated by trapping the cyclotron-produced [11C]methane in a Porapak column cooled to liquid nitrogen temperature (Landais and Finn 1989). Furthermore, it is necessary to consider possible traces of water in the target gas and in any subsequently used inert delivery gas (e.g., helium). These traces can affect the outcome and reproducibility of subsequent radiolabeling reactions but can be eliminated with a phosphorous pentoxide trap positioned before a reaction apparatus (Landais and Finn 1989).

Several secondary [11C]synthons have been developed from the primary cyclotron-produced [11C]precursors, [11C]carbon dioxide and [11C]methane (Fig. 1), with yet others emerging. These [11C]synthons enable quick, efficient, versatile, and creative 11C-labeling of functionalized molecules (Miller et al. 2008; Rotstein et al. 2013; Taddei and Gee 2018) and underpin fast progress in new PET radiotracer development. Examples of recently developed [11C]synthons are [11C]carbon disulfide for labeling organosulfur compounds (Haywood et al. 2015; Haywood et al. 2018) and [11C]fluoroform (Haskali and Pike 2017) for labeling trifluoromethyl compounds.

Fig. 1
figure1

Some established [11C]synthons. Reaction conditions: i) H2/Ni at 400 °C; ii) 1. LiAlH4, 2. H2O; iii) Ag at ~ 350 °C; iv) I2 at 700–725 °C; v) S8/sand at 500 °C or P2S5/sand at 400 °C; vi) AgOTf at 160–200 °C; vii) CoF3 at 270 °C; viii) CuCl2 on pumice at 380 °C; ix) 2% O2, Fe at 300 °C; x) NH3/Pt at 920 °C

[11C]Carbon monoxide was early described (Clark and Buckingham 1971) but its emergence as a useful radiochemical synthon was relatively slow to follow. Interest has been accelerated by major developments in mainstream transition metal-mediated carbonylation chemistry and by improvements in [11C]carbon monoxide radiosynthesis and application. This review summarizes the progress in [11C]carbon monoxide radiochemistry over the past 15 years, and covers advances in production, uses in radiolabeling, and PET radiotracer development (Fig. 1).

Advances in [11C]carbon monoxide production

‘Gas-phase’ [11C]carbon monoxide production

The earliest methods for producing [11C]carbon monoxide were based on ‘gas-phase’ reduction of cyclotron-produced [11C]carbon dioxide over activated charcoal (Fig. 2, a) or a metal surface, such as zinc or molybdenum, at a high temperature (Fig. 2, b and c). The method using activated charcoal (Clark and Buckingham 1971) results in low Am. Because PET radiotracers commonly need to be produced at high Am for efficacy and/or safety, this method is nowadays more or less obsolete.

Fig. 2
figure2

‘Gas-phase’ [11C]carbon dioxide reduction on heated columns of: a activated charcoal; b Zn; c Mo; d Zn supported on fused silica

The first system for routine production of [11C]carbon monoxide used a heated zinc column (390–400 °C) (Andersson and Långström 1995; Lidström et al. 1997). The use of pre-concentrated [11C]carbon dioxide and a recirculation unit for the reduction produced up to 70% yield and high Am typically in the 50 to 500 GBq/μmol range (Lidström et al. 1997). The generated [11C]carbon monoxide was utilized to produce 11C-labeled ketones in 36–62% isolated yields. In some settings, this method has provided near quantitative yields over many repeated runs (Dahl et al. 2015a). However, this method requires regular column maintenance and its operational success depends on the quality of the zinc. Without adequate maintenance, yields become irreproducible due to formation of zinc oxides on the metal surface over successive heating cycles. Performance may also vary unpredictably with each batch of zinc. Furthermore, the temperature required for reducing [11C]carbon dioxide (400 °C) is close to the melting point of zinc (420 °C). Therefore, accidental overheating of the zinc column must be avoided.

With the aim of developing a more convenient, efficient, and reproducible [11C]carbon monoxide synthesis, molybdenum heated to 850 °C was introduced as an alternative reductant (Zeisler et al. 1997). [11C]Carbon monoxide was obtained in up to 80% yield and with high Am (up to 555 GBq/μmol), corrected to the end of radioisotope production (ERP). The produced [11C]carbon monoxide was used to synthesize [11C]benzophenone with a non-isolated yield of up to 81%. A molybdenum column, although needing to be heated to a much higher temperature, requires less and easier maintenance than a zinc column, and gives acceptably high and more reproducible yields (up to 71%) (Dahl et al. 2015a). These features have allowed the molybdenum method to be widely adopted for the production of [11C]carbon monoxide.

Recently, the use of solid-supported zinc has been reported for improving the ‘gas-phase’ reduction of [11C]carbon dioxide to [11C]carbon monoxide (Dahl et al. 2017b). Molecular sieves, fused silica (originally in the form of silica gel), and molybdenum were investigated as solid supports with fused silica proving to be preferred. The use of a heated column of zinc supported on fused silica at 485 °C (Fig. 2, d) gave an impressive yield (93 ± 3%; n = 20). This approach overcame the main limitations of fast deactivation and potential zinc metal melting, experienced with the traditional heated zinc column. The generated [11C]carbon monoxide was tested in a 11C-carbonylation reaction yielding the corresponding 11C-labeled product in 72% yield but with a rather low Am of 11 GBq/μmol (Dahl et al. 2017b). Developments to improve the Am from this method are needed for regular radiotracer synthesis applications. Nonetheless, this advance signifies an attractive direction for developing effective ‘gas-phase’ [11C]carbon monoxide production methods.

A common requirement of these ‘gas-phase’ production methods is for fixed dedicated equipment to occupy valuable hot cell space on a long-term basis. Therefore, alternative ‘wet’ methods for [11C]carbon monoxide synthesis using portable apparatus have been sought, as follows.

‘Wet’ [11C]carbon monoxide production

Over the past 15 years, many ‘liquid-phase’ methods to produce [11C]carbon monoxide have been developed as alternatives to the ‘gas-phase’ methods. These methods aim to avoid the dedicated equipment needs of the ‘gas-phase’ methodologies and to further amplify the utility of [11C]carbon monoxide in PET radiotracer development. Examples of these ‘wet’ methods are: i) the decomposition of [11C]formyl chloride, ii) the decomposition of [11C]silacarboxylic acids, iii) the treatment of [11C]carbon dioxide with fluoride-activated disilanes, and iv) electrochemical reduction of [11C]carbon dioxide.

The production of [11C]carbon monoxide from [11C]formyl chloride was one of the first ‘wet’ methods to be described (Roeda et al. 2004). This method requires two chemical steps: 1) synthesis of [11C]formate (or [11C]formic acid) through coupling of [11C]carbon dioxide with lithium triethylborohydride in tetrahydrofuran (THF) at low temperature (ethanol-ice bath), and 2) reaction of the [11C]formate with a complex formed from hexachloroacetone and triphenylphosphine in THF at room temperature (RT) to give [11C]formyl chloride. The [11C]formyl chloride decomposes instantly to [11C]carbon monoxide (Fig. 3). With this methodology, [11C]carbon monoxide has been obtained in a very high yield (98%) but with a low Am of 9.3 GBq/μmol. Limitations of this method may be the low reproducibility of the high yield and the low Am if the system is not well maintained under an inert atmosphere. Low Am may especially arise from the contamination of prepared reagents with atmospheric carbon dioxide, especially the reactive lithium triethylborohydride. Hence, this method has not gained much traction for routine use.

Fig. 3
figure3

‘Wet’ [11C]carbon monoxide production through decomposition of [11C]formyl chloride

In non-radiochemical studies, silacarboxylic acids have been shown to degrade upon heating or in the presence of a base or a fluoride ion source to eliminate carbon monoxide (Brook 1955; Brook and Gilman 1955; Friis et al. 2011). Based on these studies, [11C]silacarboxylic acids have been explored as precursors to [11C]carbon monoxide. Lithiosilanes, prepared freshly from chlorosilanes and lithium in THF stirred at RT (either for 3 h or overnight), were found to react with [11C]carbon dioxide to yield the corresponding [11C]silacarboxylic acids. Specific [11C]silacarboxylates and [11C]silacarboxylic acids were shown to release [11C]carbon monoxide almost quantitatively within a few minutes, upon addition of tetrabutylammonium fluoride (TBAF) solution either at RT or with gentle heating (60 °C) (Fig. 4, a). The generated [11C]carbon monoxide can be transferred to a second vial with helium and used in a 11C-carbonylation reaction. Many radiolabeled compounds, mainly [11C]amides and [11C]esters, were produced in this manner. Moderate yields (> 30%) and Am in the 70–499 GBq/μmol range were achieved (Taddei et al. 2015; Nordeman et al. 2015; Bongarzone et al. 2017).

Fig. 4
figure4

‘Wet’ [11C]carbon monoxide production: a through decomposition of [11C]silacarboxylic acids; b through fluoride-activated disilanes

The use of lithiosilanes enables [11C]carbon monoxide to be produced in a simple two-vial set-up under mild reaction conditions. This overcomes the need for dedicated and fixed infrastructure in the conventional ‘gas-phase’ methods. However, limitations of this methodology are the rather lengthy preparation of fresh lithiosilane before [11C]carbon dioxide production, reagent instability, and reactivity with atmospheric carbon dioxide. These can negatively affect the [11C]carbon monoxide yield and Am. Furthermore, the requirement to add the TBAF solution after [11C]carbon dioxide capture may also be a limitation of this methodology in a routine setting.

Disilanes have been found to react with carbon dioxide in the presence of a fluoride ion source to yield the corresponding disiloxane with the elimination of carbon monoxide (Lescot et al. 2014). Disilanes are now considered to be compounds that are able to produce [11C]carbon monoxide while overcoming some of the constraints of the [11C]silacarboxylic acids methodology. 1,2-Dimethyl-1,1,2,2-tetraphenyldisilane was initially chosen for method development. Different fluoride ion sources and solvents were explored leading to TBAF and THF as the optimal activator and reaction medium for the process, respectively. Use of 0.1 equivalent of TBAF was found to be optimal for maximal [11C]carbon monoxide production. The [11C]carbon monoxide yield increased from 32% to 59% by optimizing the flow rate of the carrier gas for the delivery of [11C]carbon dioxide into the reaction system.

Other disilanes have also been investigated. 1,1,2,2-Tetramethyl-1,2-diphenyldisilane gave a maximal [11C]carbon monoxide yield of up to 74% at RT within 10 min from ERP (Fig. 4, b). The generated [11C]carbon monoxide was used in 11C-carbonylation reactions yielding radiolabeled products in high yield and with an Am in the 100–120 GBq/μmol range (Taddei et al. 2017a). For optimal Am and reproducible yield, an inert atmosphere (e.g., from a helium flow) must be maintained in the reaction vial during reagent preparation and delivery of [11C]carbon dioxide to minimize contamination from atmospheric carbon dioxide. This methodology enables the production of [11C]carbon monoxide with commercially available compounds under very mild reaction conditions and in a simple apparatus. It also eliminates: 1) the requirement for devoted infrastructure, as in the routinely used oven-based methods (Zn and Mo), and 2) time-consuming reagent preparation, as in the [11C]silacarboxylic acids method.

The first electrochemical method for producing [11C]carbon monoxide from [11C]carbon dioxide was recently described (Anders et al. 2017). This gave [11C]carbon monoxide in ≤10% yield when using electrodes with an applied potential of − 1.8 V and a Ni(cyclam)2+ or Zn(cyclen)2+ complex as an electrocatalyst under aqueous conditions (0.1 M KCl) at 20 °C (Fig. 5). One limitation of this method was the low [11C]carbon dioxide trapping efficiency. Therefore, base addition was explored for increasing the retention of [11C]carbon dioxide. 1,8-Diazabicyclo[5.4.0]undec-7-ene (DBU) or triethanolamine (TEA) raised the trapping efficiency to 80% but decreased the yield of [11C]carbon monoxide to about 3%. This decrease was attributed to an increase in the pH of the solution after the addition of base. Nevertheless, the produced [11C]carbon monoxide was used in a test carbonylation reaction and gave the 11C-labeled product with an Am of 56 GBq/μmol. Further development is needed for this method to become attractive and widespread for regular [11C]carbon monoxide production. Especially, a substantial improvement in yield is required.

Fig. 5
figure5

‘Wet’ [11C]carbon monoxide production through electrochemical reduction with Ni and Zn complexes

Advances in trapping and utilization of [11C]carbon monoxide

Major obstacles that must be surmounted for effective utilization of gaseous [11C]carbon monoxide are the generally low solubility and retention of this gas in organic solvents. Various types of apparatus have been introduced to address this issue. A system in which gaseous [11C]carbon monoxide is recirculated through the reactor was the first to be introduced (Lidström et al. 1997). The introduction of high-pressure miniature autoclaves quickly followed (Kihlberg and Långström 1999; Hostetler and Burns 2002; Kihlberg et al. 2006). If needed, these could be operated at high temperature (~ 200 °C). Such autoclaves are still in use. A high-pressure (~ 3.5 MPa) apparatus was reported to trap [11C]carbon monoxide in organic solvents with high efficiency (generally > 90%) allowing the 11C-labeling of various chemotypes in good yields (> 37%) (Dahl et al. 2015a). Moreover, loop reactors and micro-autoclaves enable efficient [11C]carbon monoxide utilization because the volume ratio of gas-phase to solution-phase within the reactor can be kept small (Eriksson et al. 2004, 2006, 2007).

A further notable advance has been the use of xenon to carry [11C]carbon monoxide efficiently into reaction media. Xenon is highly soluble in organic solvents, such as THF (Gibanel et al. 1993), and its use as delivery gas avoids undesirable build-up of pressure in the reaction vessel (Eriksson et al. 2012).

In addition to the development of improved synthesis apparatus, several compounds able to trap and then release [11C]carbon monoxide under specific conditions, such as borane-THF and copper complexes, have been used to improve the retention and utilization of [11C]carbon monoxide in solution. [11C]Boron carbonyl complexes, produced by reaction of [11C]carbon monoxide with a borane-THF complex in THF, have been found to release the [11C]carbon monoxide upon heating (Fig. 6, a) (Audrain et al. 2004). This source of concentrated [11C]carbon monoxide was used in a Pd-mediated 11C-aminocarbonylation of iodobenzene to give [11C]N-benzylbenzamide in up to 47% yield and the Pd-mediated 11C-carbonylation of 2-bromobenzyl alcohol to give [11C]isobenzofuran-1(3H)-one in up to 27% yield from trapped [11C]carbon monoxide. Trapping efficiency was high (> 90%) under the reported conditions.

Fig. 6
figure6

[11C]Carbon monoxide trapping and release: a from borane-THF complex; b from copper(I) scorpionate

Another example of a complex able to trap and release [11C]carbon monoxide is copper(I) scorpionate (copper(I) tris(pyrazolyl)borate, Cu(Tp*)). This can be formed from commercially available reagents and traps [11C]carbon monoxide from carrier streams with very high efficiency (96%). Release of [11C]carbon monoxide from this complex is also highly efficient (99%) in the presence of a competing ligand, such as triphenylphosphine (Fig. 6, b) (Kealey et al. 2009, 2014a, 2014b). The released [11C]carbon monoxide was used for 11C-carbonylation reactions in situ to give substituted [11C]amides and [11C]ureas in moderate to high yields (≥ 47%).

Advances in radiolabeling methods with [11C]carbon monoxide

[11C]Carbon monoxide is an appealing synthon for labeling a vast array of radiotracer chemotypes (Fig. 7) because of the broad versatility of modern transition-metal mediated carbonylation reactions (Brennführer et al. 2009; Gadge and Bhanage 2014; Cornilleau et al. 2015; Nielsen et al. 2018). Over the past 15 years, the radiochemistry of [11C]carbon monoxide has been widely investigated and expanded (Kealey et al. 2014b; Rahman 2015; Rotstein et al. 2016). Herein are summarized some of the latest advances in the use of [11C]carbon monoxide in Pd-mediated reactions, UV-promoted reactions, and other transition metal-mediated reactions for the synthesis of a vast variety of [carbonyl-11C]compounds (e.g., 11C-labeled ureas, amides, esters).

Fig. 7
figure7

Versatility of 11C-carbonylation reactions with [11C]carbon monoxide

[11C]Carbon monoxide has been recognized as a potentially attractive alternative to [11C]phosgene for the synthesis of [11C]ureas. Although [11C]phosgene is highly reactive and has been widely used for [11C]ureas synthesis (Roeda and Dollé 2010), the regular production of this [11C]synthon is very challenging and tedious, and often provides low Am. Pd(II)-Mediated oxidative 11C-carbonylation reactions between aliphatic or aromatic amines with [11C]carbon monoxide have been explored recently for the synthesis of symmetrical and unsymmetrical [11C]ureas (Roslin et al. 2017) (Fig. 8, a). Xenon was used to carry the [11C]carbon monoxide into a septum-sealed glass reactor. [11C]Ureas were obtained in yields up to 61% and with high Am in the 247–319 GBq/μmol range, under quite mild conditions (≤ 120 °C, 10 min).

Fig. 8
figure8

Pd-Mediated 11C-carbonylations producing a [11C]ureas; b [11C]acrylamides; c [11C]amides, [11C]esters, [11C]carboxylic acids, an [11C]aldehyde, and a [11C]ketone

Pd-Mediated carbonylation reactions have featured in the 11C-carbonylation of several other chemotypes. Functionalized [11C]acrylamides have been prepared through Pd(0)-mediated carbonyl insertions between 4-anilino-6-aminoquinazoline and substituted vinyl iodides in a high-pressure micro-autoclave (Fig. 8, b). The desired [11C]acrylamides were obtained in 24–61% yields and with an Am of 60 GBq/μmol (Åberg and Långström 2012).

In another study, Pd(II)-mediated reactions were used to synthesize [11C]amides, a [11C]ester, a [11C]carboxylic acid, a [11C]aldehyde, and a [11C]ketone at atmospheric pressure under thermal heating (Fig. 8, c) (Dahl et al. 2013). The catalyst, Pd2(π-cinnamyl)Cl2, was paired with the ligand Xantphos. This ligand performed better than others, probably because of its wide bite angle. Microwave heating improved the yields in the reactions of electron-deficient aryl halides. Furthermore, this methodology has also been used with aryl chlorides as substrates to produce an [11C]arylcarboxylic acid and [11C]aryl esters (Dahl et al. 2014).

Another approach for producing [11C]amides is a quick (5 min) one- or two-pot Pd-mediated 11C-carbonylation reaction between an aryl halide and a lithiated amine that has been freshly prepared from amine and n-butyllithium. Eleven [11C]amides were obtained in isolated yields of 18–72% (Fig. 9) (Itsenko et al. 2007). This method extends the scope of Pd-mediated 11C-carbonylation reactions to the use of weakly nucleophilic amines.

Fig. 9
figure9

Synthesis of [11C]amides from lithiated amines through Pd-mediated 11C-carbonylation

11C-Carbonylation of aryl, heteroaryl, allyl, and alkyl boronic acid pinacol esters in the presence of p-benzoquinone and triphenylphosphine has been investigated for producing a variety of [11C]methyl esters. Yields in the 6–80% range were obtained from quick reactions (5 min) performed under atmospheric pressure with gentle heating (Fig. 10) (Ishii et al. 2015). The conversion of [11C]methyl esters into the corresponding [11C]carboxylic acids or [11C]amides was easily achieved through treatment with sodium hydroxide or aqueous ammonium, respectively (Fig. 10). This approach was also applied to the synthesis of [carbonyl-11C]aspirin and [carbonyl-11C]salicylic acid in yields of 15 ± 2% and 58 ± 5%, respectively. These yields surpass those obtained via the direct 11C-carboxylation of Grignard reagents (Sasaki et al. 1999).

Fig. 10
figure10

Synthesis of [11C]methyl esters through Pd(0)-mediated 11C-carbonylation of boronic acid pinacol esters. PBQ = p-benzoquinone

Diaryliodonium salts have featured prominently as reactive precursors for radiohalogenation reactions (Pike 2018; Telu et al. 2019). They are also known to undergo Pd(II)-mediated carbonyl insertion with [11C]carbon monoxide (Al-Qahtani and Pike 2000). A two-pot procedure for the rapid Pd(0)-mediated 11C-carbonylation of aryl(mesityl)iodonium salts at RT and low pressure has been recently developed (Fig. 11) (Altomonte et al. 2017). A range of non-mesityl [11C]aryl carboxylic acids and [11C]aryl amides bearing an electron-withdrawing or electron-donating group were obtained in up to 71% yield within 2 min at RT.

Fig. 11
figure11

Syntheses of [11C]aryl carboxylic acids and [11C]aryl amides through 11C-carbonylation of diaryliodonium salts

[11C]Amides, [11C]esters, [11C]carboxylic acids, and [11C]aldehydes have also been prepared via a two-step methodology, composed of: 1) in situ generation of a [11C]benzoyl acid chloride through a Pd(0)-mediated 11C-carbonylation of an aryl iodide, and 2) subsequent reaction of the generated [11C]benzoyl acid chloride with a chosen nucleophile in a second reaction vial (Fig. 12, a) (Dahl and Nordeman 2017). Many diverse 11C-labeled products were obtained within 7 min in 41–93% yields. This methodology has been successfully extended to the synthesis of [11C]benzyl alcohols, [11C]benzaldehydes, and [11C]phenyl ketones (Fig. 12, b) (Roslin et al. 2018) and is an attractive alternative to a former method for accessing [11C]aryl acid chlorides based on the 11C-carboxylation of Grignard reagents (Pike et al. 1982; Krasikova et al. 2009).

Fig. 12
figure12

Two-step methodology for the synthesis of a [11C]amides, [11C]esters, [11C]carboxylic acids, and [11C]aldehydes; b [11C]benzyl alcohols, [11C]benzaldehydes, and [11C]phenyl ketones via [11C]benzoyl acid chlorides

Pd-Mediated 11C-carbonylation reactions have been performed in novel experimental set-ups (Fig. 13). A microfluidic reactor with a solution containing [11C]carbon monoxide in the form of its complex with copper(I) scorpionate (Cu(Tp*)) was applied to the synthesis of a model [11C]amide, namely [11C]N-benzylbenzamide. [11C]N-Benzylbenzamide was obtained with a radiochemical purity of 69% under a microreactor temperature of 200 °C and with a flow rate of 20 μL/min per syringe, corresponding to a very short residence time (< 1 min) in the microreactor (Fig. 13, a) (Kealey et al. 2011). [11C]Dibenzylurea was formed as a significant byproduct (22 ± 1%).

Fig. 13
figure13

Pd-Mediated syntheses of: a [11C]amides in a microfluidic reactor; b [11C]amides and [11C]esters in a gas-liquid microfluidic platform

The first application of a gas-liquid segmented-flow microfluidic platform was described for the Pd-mediated synthesis of a variety of 11C-labeled compounds (e.g., [11C]amides and [11C]esters) (Fig. 13, b). The 11C-labeled compounds were obtained in good yields (≥ 38%) and with Am in the 40–54 GBq/μmol range (Dahl et al. 2015b). These yields supersede those from a previously reported gas-liquid annular flow microfluidic apparatus (Miller et al. 2011). This improvement was attributed to the larger gas-liquid interface in the segmented-flow because of the continuous formation of microbubbles (Dahl et al. 2015b).

Generally, Pd-mediated carbonylation reactions are limited to compounds that lack β-hydrogens on saturated sp3 carbons, such as aryl halides or triflates. Compounds with β-hydrogens do not produce carbonylated products with carbon monoxide because the β-hydride elimination from σ-alkyl-Pd intermediates is competitive and usually predominant (Fig. 14). To circumvent this issue, a high-pressure reactor with a quartz window open to light from a mercury lamp has been used successfully for radical 11C-carbonylations of substrates bearing β-hydrogen atoms (Fig. 15, a) (Itsenko et al. 2004). Radical 11C-carbonylation has also been improved recently by using a low-pressure xenon-[11C]carbon monoxide delivery unit and azobisisobutyronitrile (AIBN) as a radical initiator under thermal conditions (Fig. 15, b). This approach showed broad substrate compatibility with alkyl iodides containing β-hydrogen atoms. The desired [11C]amides were obtained in 9–25% isolated yields and with an Am of 101 ± 13 GBq/μmol (Chow et al. 2016).

Fig. 14
figure14

Representation of β-elimination mechanism

Fig. 15
figure15

Radical 11C-cabonylation of substrates with β-hydrogens: a at high pressure; b at ambient pressure

Transition-metals other than palladium have been used to mediate 11C-carbonylation reactions. One example is the nickel(0)-mediated 11C-carbonylation on non-activated alkyl iodides bearing a β-hydrogen atom (Fig. 16). [11C]Alkyl amides were obtained in 33–57% yields within 5 min when using tert-butanol as solvent (Rahman et al. 2016). However, this method requires handling of the nickel catalyst under an argon atmosphere to avoid any reagent oxidation that would result in failure of the radiolabeling reaction.

Fig. 16
figure16

Ni(0)-Mediated cross-coupling reaction of non-activated alkyl iodides bearing a β-hydrogen atom. Ni(COD)2 = bis(1,5-cyclooctadiene)nickel(0)

Substituted [11C]ureas and [11C]sulfonyl ureas have been synthesized through rhodium(I)-mediated insertion of [11C]carbon monoxide between azides and amines (Fig. 17, a) (Åberg and Långström 2011). This method shows good functional group tolerance. The radiolabeled products were obtained under mild conditions in 14–96% yields and with an Am in the 100–600 GBq/μmol range.

Fig. 17
figure17

Rh(I)-Mediated 11C-carbonylations producing: a [11C]ureas and [11C]sulfonyl ureas; b [11C]sulfonyl carbamates. [Rh(COD)Cl]2 = chloro(1,5-cyclooctadiene)rhodium(I) dimer

A Rh(I)-mediated multicomponent reaction between sulfonyl azides, alcohols, and [11C]carbon monoxide has been reported for producing [11C]sulfonyl carbamates (Fig. 17, b) (Stevens et al. 2016). This method is compatible with structurally diverse sulfonyl azides. Various [11C]sulfonyl carbamates were produced in good yields (33–88%).

[11C]Carbon monoxide for PET radiotracer development

As a result of the major advances that have taken place in [11C]carbon monoxide production and radiochemical utility, 11C-labeled carbonyl groups have become increasingly prevalent in candidate PET radiotracer designs (Rotstein et al. 2016). The possibility to radiolabel carbonyl groups is of extreme relevance for using PET in drug distribution studies, where the unchanged drug structure needs to be radiolabeled and where motifs, such as radiolabeled methyl groups or fluorine atoms, cannot be introduced.

Although many applications of [11C]carbon monoxide require the presence of transition metals (e.g., Pd) as reagents for 11C-carbonylations, such metals have not been an issue for producing radiotracers under CGMP conditions. For example, [11C]UCB-J and [11C]FPEB have been produced for human use through Pd-mediated labeling reactions (Lohith et al. 2014; Nabulsi et al. 2016; Lohith et al. 2017; DiFilippo et al. 2019). In our laboratory, we have found that normal methods for radiotracer purification (e.g., HPLC) are capable of reducing Pd residues to sub-ppb levels (e.g., for the [11C]FPEB synthesis (Lohith et al. 2014)). These levels are well below the limits considered acceptable for parental administration of non-radiopharmaceutical drugs (1 ppm per day; (USP 2017).

Additional considerations pertain to 11C-carbonylation reactions for PET radiotracer synthesis, such as steric constraints adjacent to carbonylation sites, functional group tolerance in more structurally elaborate substrates, and ease of product purification. Moreover, the overall efficiency of the production process of a PET radiotracer, from cyclotron radioisotope production to the radiochemically pure product ready for intravenous injection, is also an important aspect to consider. Ideally, the process should be readily amenable to automation. Henceforth, we summarize the progress that has been made towards producing PET radiotracers through reactions with [11C]carbon monoxide.

The microfluidic apparatus described earlier has been tested for the syntheses of PET radiotracers. The liquid-liquid phase microreactor featuring the copper complex (Cu(Tp*)), was implemented to synthesize a 11C-labeled neuropeptide Y Y5 receptor antagonist, [11C]MK-0233 (Table 1, Entry 1). [11C]MK-0233 was produced through a Pd-mediated 11C-carbonylation reaction with [11C]carbon monoxide. [11C]Carbon monoxide was released from a Cu(Tp*)11CO complex for an internal cyclization reaction to give [11C]MK-0233 (Fig. 18, a). [11C]MK-0233 was obtained ready for intravenous injection in a yield of 7.1 ± 2.2% from utilized [11C]carbon monoxide and with an Am of 100 ± 15 GBq/μmol at 27 min from ERP (Kealey et al. 2011).

Table 1 Radiotracers radiolabeled using [11C]carbon monoxide, the corresponding biological target, and disease relevance
Fig. 18
figure18

Synthesis of a [11C]MK-0233; b [carbonyl-11C]raclopride, and [carbonyl-11C]FLB 457

[O-methyl-11C]Raclopride and [O-methyl-11C]FLB 457 are well known radiotracers for PET imaging of human striatal and extrastriatal D2/D3 receptors, respectively (Ito et al. 1999; Okubo et al. 1999). [Carbonyl-11C]raclopride and [carbonyl-11C]FLB 457 (Entries 2 and 3) have been synthesized in a gas-liquid phase segmented microreactor through Pd-mediated 11C-carbonylation reactions (Fig. 18, b). High non-isolated yields of [carbonyl-11C]raclopride and [carbonyl-11C]FLB 457 (79 ± 1% and 61 ± 4%, respectively) were obtained from utilized [11C]carbon monoxide (Dahl et al. 2015b). A series of model [carbonyl-11C]amides were produced similarly in the same study and had moderate Am (40–54 GBq/μmol).

[carbonyl-11C]Raclopride has also been produced through a one-step 11C-carbonylation reaction at atmospheric pressure using Pd2(π-cinnamyl)Cl2 as catalyst and Xantphos as supporting ligand (Rahman et al. 2015). The yield of [carbonyl-11C]raclopride ready for intravenous injection was 50 ± 5% from [11C]carbon monoxide trapped in the reaction vial and the Am was 34 GBq/μmol. The trapping efficiency was moderate (65 ± 5%). PET experiments were performed in monkey to compare [carbonyl-11C]raclopride and [O-methyl-11C]raclopride. A key question in this study was whether the change in radiolabeling position from O-methyl to carbonyl would be detrimental to [11C]raclopride performance in vivo. However, the PET experiments showed remarkably similar results with regards to protein binding, emergence of radiometabolites in plasma, and quantitative outcome measures of D2/D3 receptors (Rahman et al. 2015). This similarity argues against demethylation as being a prime route of radiotracer metabolism; more likely is that amide hydrolysis occurs.

It has been reasoned that two potentially slow steps in the Pd-mediated 11C-carbonylation of aryl halides (Fig. 19), namely activation of the Pd catalyst and subsequent oxidative addition of the aryl halide, can be beneficially avoided by pre-synthesis and isolation of the desired Pd-aryl oxidative addition complex (Aryl)Pd(X)Ln (Andersen et al. 2015). This was confirmed with the 11C-aminocarbonylation of an isolated Pd-aryl oxidative addition complex, formed from Pd(dba)2 and Xantphos, for the synthesis of [carbonyl-11C]raclopride (Fig. 20) (Andersen et al. 2015). This radiotracer was obtained in 38–44% yield from trapped [11C]carbon monoxide within 8 min from ERP and with a high Am in the 333–407 GBq/μmol range. An advantage of this radiosynthetic approach was that it gave [carbonyl-11C]raclopride in high initial purity (94 ± 1%) thereby reducing separation challenge. Two other potential radiotracers, [carbonyl-11C]olaparib and a neuropeptide Y Y3 receptor antagonist, [carbonyl-11C]JNJ 31020028, were also efficiently radiolabeled from pre-formed Pd-complexes.

Fig. 19
figure19

Pd-Mediated 11C-carbonylation of aryl halides

Fig. 20
figure20

Use of a pre-formed Pd-aryl oxidative addition complex for the synthesis of [carbonyl-11C]raclopride

The synthesis of selective and potent PET radiotracers for translocator protein (TSPO), a biomarker for neuroinflammation, is of continuing major interest (Dupont et al. 2017). The TSPO radioligand, [O-methyl-11C]DAA1106 (Zhang et al. 2003), has found quite wide application in clinical research. The 11C-labeling of DAA1106, and some of its analogues has been achieved through Pd-mediated 11C-aminocarbonylation reactions. A strong base, such as n-butyllithium, was used to activate the amine and improve the overall yields. [carbonyl-11C]DAA1106 (Entry 4) was produced in 30% yield and with a high Am of 455 GBq/μmol at 36 min after ERP (Fig. 21, a) (Rahman and Långström 2007). Analogues were also obtained in moderate yields and with high Am. Investigations are needed to compare the behavior of [carbonyl-11C]DAA1106 with that of the established [O-methyl-11C]DAA1106, especially to test whether PET imaging performance might differ because of potential differing radiometabolite profiles.

Fig. 21
figure21

Synthesis of a [11C]DAA1106 and b [11C]PipISB via Pd-mediated 11C-aminocarbonylation reactions

The development of PET radiotracers for imaging brain cannabinoid subtype-1 (CB1) receptors has been pursued because of the implication of this receptor in a range of neuropsychiatric disorders, such as schizophrenia, anxiety, and depression. [11C]PipISB, a selective and high affinity CB1 receptor radioligand (Entry 5) has been produced ready for intravenous injection within 44 min from ERP through a Pd-mediated 11C-aminocarbonylation reaction between an aryl iodide precursor and [11C]carbon monoxide in overall yields of 3–12% from [11C]carbon monoxide and with an Am in the 21–67 GBq/μmol range (Fig. 21, b) (Donohue et al. 2008). This radiotracer was studied and compared with the 18F-labeled version in monkey. Each radiotracer gave high proportions of CB1 specific binding in brain (Finnema et al. 2009). However, neither [11C]PipISB nor [18F]PipISB has been advanced to human study because other higher-performing radiotracers for PET imaging of human brain CB1 receptors appeared concurrently (e.g., [11C]MePPEP (Terry et al. 2009) and [18F]FMPEP-d2 (Terry et al. 2010)).

Changes in brain cholinergic neurons are implicated in several neurodegenerative disorders. Vesicular acetylcholine transporter (VAChT) is considered a biomarker for cholinergic neurons. The need of potential PET radiotracers for VAChT has been addressed in the synthesis of a small library ([11C]5a − f, Fig. 22) of potential VAChT radiotracers (Bergman et al. 2014). Six piperazine-based radiotracers were produced through Pd-mediated 11C-carbonylation insertions between synthesized amines and commercially available aryl iodides in isolated yields of 4–25% from starting radioactivity and with Am in the 124–597 GBq/μmol range. Two of these radiotracers exhibited specific binding to VAChT in vitro. However, these results did not support further evaluation of these radiotracers in pre-clinical PET imaging.

Fig. 22
figure22

11C-Labeling with [11C]carbon monoxide of potential VAChT radiotracers

The metabotropic glutamate subtype-1 receptor (mGluR1) has been implicated in anxiety and mood disorders. Therefore, mGluR1 has gained interest as a potential drug target. A Pd-mediated [11C]carbon monoxide insertion reaction has been used to produce [11C]FIMX as a potential PET radiotracer for imaging brain mGluR1 (Hong et al. 2015). [11C]FIMX (Entry 6), was synthesized through a two-pot procedure, composed of Pd-mediated carbonylation of 1-fluoro-4-iodobenzene with [11C]carbon monoxide in a first pot and subsequent treatment of the [11C]acylpalladium complex with Boc-protected amine precursor followed by Boc removal in a second pot (Fig. 23). [11C]FIMX was obtained ready for intravenous injection in an overall yield of about 5% from initial cyclotron-produced radioactivity and with an Am of 102 ± 31 GBq/μmol at 42 ± 3 min from ERP. PET imaging in monkey showed [11C]FIMX to be an effective radiotracer for mGluR1 and to have imaging properties very similar to those of [18F]FIMX (Hong et al. 2015; Xu et al. 2013). A potential advantage of the 11C-label over the 18F-label is the possibility for two PET measurements in a single subject in 1 day. This possibility is convenient for drug occupancy studies which may require a series of paired baseline and pharmacological challenge experiments in single subjects.

Fig. 23
figure23

Two-pot synthesis of [11C]FIMX

The synthesis of PET radiotracers for histone deacetylase 6 (HDAC6) has gained interest because of the possible involvement of this enzyme in cancer and various neuropsychiatric disorders (Wey et al. 2016). A hydroxamic group features prevalently in many inhibitors of this enzyme. The HDAC6 inhibitor, tubastatin A, has been radiolabeled with carbon-11 (Entry 7) in the hydroxamic acid group via a two-step process (Fig. 24), namely: 1) Pd-mediated [11C]carbon monoxide insertion between an aryl iodide and p-nitrophenol, and 2) ultrasound-assisted hydroxyaminolysis of the pre-formed [11C]ester with excess hydroxylamine in the presence of the strong phosphazene base P1-t-Bu (Lu et al. 2016). [11C]Tubastatin A was obtained ready for intravenous injection in a yield of 16 ± 6% (n = 4) from initial cyclotron-produced [11C]carbon dioxide and with a low Am of 7.4 GBq/μmol at 61 min from ERP. However, attempts to apply this radiolabeling approach to other hydroxamic acids were unsuccessful because the final hydroxyaminolysis step gave only the corresponding [11C]carboxylic acids.

Fig. 24
figure24

Radiosynthesis of [11C]tubastatin A. P1-t-Bu = tert-butylimino-tris(dimethylamino)phosphorene

Novel PET radiotracers for the diagnosis and monitoring of Alzheimer’s disease are a constant demand in the PET neuroimaging field. An hydroxyethylamine-based inhibitor for enzyme β-secretase 1 (BACE-1), namely [11C]BSI-IV (Entry 8), has been labeled through a Pd-mediated 11C-aminocarbonylation reaction on an aryl halide precursor (Fig. 25) as a potential useful PET radiotracer for evaluating Alzheimer’s disease in vivo. [11C]BSI-IV was produced in isolated yields of 29 ± 12% (n = 12) from trapped [11C]carbon monoxide and with a high Am of 790 ± 155 GBq/μmol (Nordeman et al. 2014). Trapping efficiency was about 60%. Biodistribution and PET-CT studies were performed on healthy rat brain sections and male rats, respectively. However, these studies showed low specific binding of [11C]BSI-IV to BACE-1 in vitro, fast clearance in vivo, and low uptake in brain. These results proved [11C]BSI-IV to be an unpromising PET radiotracer for BACE-1.

Fig. 25
figure25

Synthesis of [11C]BSI-IV through a Pd-mediated 11C-aminocarbonylation reaction

The serotonin subtype 1B (5-HT1B) receptor is involved in migraine, depression, and anxiety. [11C]Carbon monoxide has been implemented for radiolabeling AZ11136118, a high-affinity full 5-HT1B receptor agonist. [11C]AZ11136118 (Entry 9) was obtained ready for intravenous injection in 6.4 ± 1.6% overall yield from starting [11C]carbon dioxide and with an Am of 83 ± 51 GBq/μmol (n = 7) within 50 min from ERP through a two-pot Pd-mediated 11C-carbonylation reaction (Fig. 26, a) (Lindberg et al. 2019). In this study, another high-affinity full 5-HT1B receptor agonist, [11C]AZ11895987, was synthesized using [11C]methyl triflate for an N-methylation reaction. PET imaging with the two radiotracers were performed in monkeys to investigate how the intrinsic activity of full 5-HT1B receptor agonist radiotracers might affect PET imaging outcomes. However, both [11C]AZ11136118 and [11C]AZ11895987 exhibited too low brain uptake to be useful PET radiotracers (Lindberg et al. 2019).

Fig. 26
figure26

11C-Labeling of a [11C]AZ11136118 and b [11C]LSN3316612 with [11C]carbon monoxide

Very recently Lu et al. presented the development of effective radiotracers for PET imaging of the enzyme O-GlcNAcase (OGA), a potential biomarker and therapeutic target for tauopathy (Lu S, Haskali MB, Ruley KM, Dreyfus NJ-F, DuBois SL, Soumen P, et al. Discovery and development of 18F- and 11C-labeled LSN3316612 as positron emission tomography radioligands for quantifying O-linked-β-N-acetyl-glucosamine hydrolase in brain, submitted). [3H]LSN3316612 had shown high selectivity and potency for binding to OGA in post mortem brains of rat, monkey, and human. The radiotracer, [11C]LSN3316612 (Entry 10), was produced for intravenous injection with a two-step procedure through Pd-mediated 11C-carbonylation reaction (Fig. 26, b) in a low overall yield (3.5 ± 1.3%) from cyclotron-produced [11C]carbon dioxide and with an Am of 74 ± 39 GBq/μmol at 50 min after ERP. Evaluation of [11C]LSN3316612 with PET in monkey demonstrated that this radiotracer is effective for quantifying OGA in monkey brain.

The histamine type-3 receptor (H3R) has been considered a drug target for treating neuropsychiatric disorders. Novel amide ligands for H3R, have been radiolabeled through Pd-mediated 11C-aminocarbonylation reactions and evaluated with PET in monkeys (Dahl et al. 2018). [carbonyl-11C]AZ13153556, [carbonyl-11C]AZD5213, and [carbonyl-11C]AZ13198083 were obtained in good non-isolated yields (≥ 80%) from starting [11C]carbon monoxide and with a moderate Am in the 19–28 GBq/μmol range at time of intravenous injection (Dahl et al. 2018). [carbonyl-11C]AZ13198083 (Entry 11) (Fig. 27, a) had first been reported in 2013 (Dahl et al. 2013). In the later study, [carbonyl-11C]AZ13198083 was found to be the most promising of this candidate triad for potential PET imaging of H3R in living human subjects.

Fig. 27
figure27

Synthesis of potential H3R radiotracers: a [11C]AZ13198083; b [11C]1–4; c 11C-labeling of [11C]vemurafenib; and d of an sHE inhibitor [11C]19 through Pd-mediated 11C-carbonylation reactions

Structurally elaborate [11C]aryl ketones, [11C]1–4 (Entry 12) (Fig. 27, b), have been produced through Pd-mediated [11C]carbon monoxide insertion reactions between aryl iodides and aryltributylstannanes as other examples of potential PET radiotracers for imaging brain H3R. [11C]1–4 were obtained for pre-clinical use in 5–9% yields from cyclotron-produced [11C]carbon dioxide and with Am ≥ 115 GBq/μmol (Siméon et al. 2017). However, these radiotracers were not advanced to evaluation with PET.

Recently, vemurafenib (Entry 13), a serine/threonine kinase inhibitor, has been radiolabeled with carbon-11 to assess its potential as a PET radiotracer for tumors expressing the BRAFV600E mutation. Labeling was achieved through Pd-mediated 11C-carbonylation in 21 ± 4% yield from [11C]carbon monoxide and with an Am of 55 ± 18 GBq/μmol (Fig. 27, c) (Slobbe et al. 2017). However, preliminary in vitro and biodistribution studies in mice suggested that [11C]vemurafenib is not a suitable PET radiotracer for identifying the BRAFV600E mutation in vivo (Slobbe et al. 2017).

The development of PET radiotracers for soluble epoxide hydrolase (sHE), an enzyme implicated in inflammation and neuropathic pain (Shen and Hammock 2012), may be of interest to further understand these pathophysiological processes. Recently, a urea sHE inhibitor, [11C]19 (Entry 14), has been radiolabeled through a Pd-mediated oxidative 11C-carbonylation between an aryl amine and an aliphatic amine (Fig. 27, d). [11C]19 was obtained in 41 ± 7% yield from [11C]carbon monoxide and with an Am in the 247–319 GBq/μmol range at 41–43 min from ERP (Roslin et al. 2017). However, evaluation of this radiotracer with PET imaging has not been reported.

Pd-Mediated 11C-aminocarbonylation reactions with [11C]carbon monoxide have been exploited to generate substituted [11C]acrylamides as candidate PET radiotracers for active tissue transglutaminase (TG2) (Fig. 28) (van der Wildt et al. 2016). Three 11C-labeled inhibitors ([11C]1–3) for TG2 were produced in 38–55% isolated yields from starting [11C]carbon monoxide and with Am ≥ 202 GBq/μmol. Ex vivo biodistribution and plasma stability studies were performed in healthy Wistar rats. One of the radiotracers, [11C]3 (Entry 15), showed high metabolic stability, low brain uptake, and selective binding to TG2 in tumor sections (van der Wildt et al. 2016).

Fig. 28
figure28

Pd-Mediated 11C-carbonylation approach used to 11C-label potential TG2 inhibitors

Glutamic acid decarboxylase (GAD) is the major enzyme responsible for the synthesis of the neurotransmitter γ-aminobutyric acid (Fenalti et al. 2007) and dysregulation of GAD function has been implicated in neuropsychiatric disorders, such as schizophrenia (Akbarian and Huang 2006). Attempts have been made to develop PET radiotracers for GAD to potentially elucidate its function in neuropsychiatric disorders. [11C]MPATB (Entry 16), a 11C-labeled analogue of the GAD inhibitor 3-mercaptopropionic acid, has been synthesized through a one-pot Pd-mediated 11C-carbonylation on vinyl iodide in the presence of tert-butanol and subsequent thiol-ene reaction with thiobenzoic acid (Fig. 29). [11C]MPATB was produced in high radiochemical purity of 99% and an isolated yield of 0.5–3% from starting [11C]carbon monoxide (Taddei et al. 2017b; Taddei C, Filp U, Pekošak A, Poot AJ, Windhorst AD, Gee AD. Synthesis of a 11C-tracer for potential brain glutamic acid decarboxylase (GAD) targeting, submitted). Further development to improve the overall yield of [11C]MPATB may be required.

Fig. 29
figure29

Synthesis of [11C]MPATB through Pd-mediated 11C-carbonylation followed by a thiol-ene reaction

[11C]Carbon monoxide has also been used with vinyl iodide to produce racemic side-chain labeled 11C-labeled amino acids, namely the neurotransmitter glutamate and the essential amino acid glutamine through a three-step approach: 1) Pd-mediated 11C-carbonylation of vinyl iodide in the presence of tert-butanol or tritylamine, followed by 2) Michael addition reactions with the generated [11C]acrylate and [11C]acrylamide, respectively, and finally 3) acid hydrolysis (Fig. 30) (Filp et al. 2017). The initial 11C-carbonylation reactions proceeded in high yields under preferred conditions (> 70%). The Michael addition reaction was moderately efficient when using CsOH·H2O as base for the synthesis of [11C]glutamine. Furthermore, introduction of a chiral phase-transfer catalyst resulted in some stereoselectivity for the Michael addition reaction.

Fig. 30
figure30

Synthesis of [11C]glutamate and [11C]glutamine via Pd-mediated 11C-carbonylation followed by Michael addition reactions

Four carboxylic acid-type high-affinity inhibitors, [11C]1–4 (Entry 17), of cytosolic phospholipase A2α (cPLA2α), an enzyme implicated in neuroinflammatory conditions, have been radiolabeled through Pd-mediated 11C-carbonylation on aryl iodide precursors followed by hydrolysis (Fig. 31). The desired 11C-labeled inhibitors were obtained ready for intravenous injection in 1.1–5.5% overall yields from [11C]carbon dioxide and with an Am in the 70–141 GBq/μmol range (Fisher et al. 2018). These candidate radiotracers were evaluated with PET imaging in mice. However, they proved to be ineffective PET radiotracers because of their low entry and retention in brain following intravenous administration.

Fig. 31
figure31

11C-Labeling approach used for four candidate inhibitors of cPLA2α

Transition-metals other than palladium have been used to mediate 11C-carbonylation reactions for the synthesis of candidate PET radiotracers. One example is the Rh-promoted 11C-carbonylation reaction with ethyl diazoacetate and ethanol to produce [carbonyl-11C]diethyl malonate (Entry 18) in an isolated yield of 20 ± 7% from starting radioactivity and with an Am of 127 GBq/μmol at EOS (Fig. 32, a) (Barletta et al. 2006). This difunctional labeling synthon was then alkylated to produce [carbonyl-11C]diethyl 2,2-diethylmalonate in 50% yield as a candidate PET radiotracer. This approach is promising for the synthesis of different [carbonyl-11C]malonates as potential PET radiotracers (Barletta et al. 2006).

Fig. 32
figure32

Rh-Mediated synthesis of: a [11C]diethyl malonate; b an agonist of AT2R, [11C]21; c [11C]phenytoin; d [11C]sorafenib

A Rh-mediated 11C-carbonylation reaction has also been used to synthesize a 11C-labeled agonist of non-peptide angiotensin II subtype 2 receptor (AT2R), a receptor involved in prostate cancer. This radiotracer, namely [11C]C21 (Entry 19), was obtained from a sulfonyl azide in a multicomponent reaction in 24 ± 10% yield from [11C]carbon monoxide and with an Am in the 34–51 GBq/μmol range (Fig. 32, b), and then evaluated with PET in rats (Stevens et al. 2016). [11C]C21 bound specifically to prostate tumor cells expressing AT2R but showed rapid metabolism and excretion, limiting its use as a PET radiotracer. Nevertheless, these findings provide an impetus for further development of AT2R-selective PET radiotracers.

Rh-Promoted 11C-carbonylations have also been used to synthesize a candidate radiotracer for the efflux transporter P-glycoprotein (P-gp) in rats, namely [11C]phenytoin (Fig. 32, c; Entry 20). [11C]Phenytoin was produced ready for intravenous injection in an overall yield of 22 ± 4% and with an Am of 277 ± 67 GBq/μmol at the end of synthesis (Verbeek et al. 2012). However, PET evaluation in rats showed [11C]phenytoin to be a weak Pg-p substrate. Another example is the Rh-mediated synthesis of [urea-11C]sorafenib (Fig. 32, d; Entry 21), a 11C-labeled tyrosine kinase inhibitor. [urea-11C]Sorafenib was obtained ready for intravenous injection in a yield of 27% ± 11% and with an Am of 30–50 GBq/μmol within 50 min (Poot et al. 2013). [urea-11C]Sorafenib proved to be stable in in vivo. This may encourage future PET studies for tumor targeting in tumor-bearing mice.

Selenium-mediated 11C-carbonylation reactions were early described for the synthesis of functionalized [11C]carbamates (Kihlberg et al. 2002). More recently, [carbonyl-11C]Zolmitriptan (Entry 22), a serotonin 5-HT1B/1D receptor agonist, has been prepared in an autoclave through a Se-mediated [11C]carbon monoxide insertion reaction (Fig. 33) (Lindhe et al. 2011). [11C]Zolmitriptan was used to map binding sites in monkey brain and to characterize the regional distribution of zolmitriptan binding to 5-HT1 receptor subtypes. This PET radiotracer showed a high proportion of binding (90%) to high-affinity sites and a discrete regional distribution in monkey brain (Lindhe et al. 2011).

Fig. 33
figure33

Radiosynthesis of [11C]zolmitriptan through Se-mediated 11C-carbonylation

Conclusions

[11C]Carbon monoxide continues to emerge as a useful radiolabeling synthon for introducing the [11C]carbonyl group into a wide variety of chemotypes. It can be prepared and utilized efficiently, and high Am can be achieved. The radiolabeling reactions with [11C]carbon monoxide show high functional group tolerance providing avenues towards attractive single-step PET radiotracer production. Furthermore, the metabolic hydrolysis of PET radiotracers that are 11C-labeled in an ester or amide carbonyl site can lead to poorly brain-penetrant radiometabolites, such as the corresponding [11C]carboxylic acids. This is especially relevant and useful for avoiding PET signal contamination when PET imaging is performed with radiotracers targeting brain proteins.

Candidate PET radiotracers produced from [11C]carbon monoxide have so far advanced only to evaluation in animals. It may be expected that in time some candidate PET radiotracers, synthesized through 11C-carbonylation reactions, will justify their production for PET imaging in human subjects. To encourage further progress in this direction, there is a need for commercially available automated radiosynthesis apparatus specific for [11C]carbon monoxide production and utility and which provides a high level of consistent performance, especially in a CGMP (current good manufacturing practice) environment.

Availability of data and materials

Not applicable.

Abbreviations

5-HT1B :

5-Hydroxytryptamine 1B

AIBN:

Azobisisobutyronitrile

A m :

Molar activity

AT2R:

Angiotensin II subtype 2 receptor

BACE-1:

β-Secretase 1

BBB:

Blood-brain barrier

CB1 :

Cannabinoid type-1

CGMP:

Current good manufacturing practice

cPLA2α:

Cytosolic phospholipase A2α

DBU:

1,8-Diazabicyclo [5.4.0]undec-7-ene

EOS:

End of synthesis

ERP:

End of radioisotope production

GAD:

Glutamic acid decarboxylase

H3R:

Histamine type-3 receptor

HDAC6:

Histone deacetylase 6

mGluR1:

Metabotropic glutamate receptor 1

OGA:

O-GlcNAcase

PET:

Positron emission tomography

P-gp:

P-Glycoprotein

RT:

Room temperature

sHE:

Soluble epoxide hydrolase

TBAF:

Tetrabutylammonium fluoride

TEA:

Triethanolamine

TG2:

Tissue transglutaminase

THF:

Tetrahydrofuran

TKI:

Tyrosine kinase inhibitor

TSPO:

Translocator protein

VAChT:

Vesicular acetylcholine transporter

References

  1. Åberg O, Långström B. Synthesis of substituted [11C]ureas and [11C]sulphonylureas by Rh(I)-mediated carbonylation. J Label Compd Radiopharm. 2011;54(1):38–42.

  2. Åberg O, Långström B. Combinatorial synthesis of labelled drugs and PET tracers: synthesis of a focused library of 11C-carbonyl-labelled acrylamides as potential biomarkers of EGFR expression. J Label Compd Radiopharm. 2012;55(14):477–83.

  3. Akbarian S, Huang H-S. Molecular and cellular mechanisms of altered GAD1/GAD67 expression in schizophrenia and related disorders. Brain Res Rev. 2006;52(2):293–304.

  4. Al-Qahtani MH, Pike VW. Palladium(II)-mediated 11C-carbonylative coupling of diaryliodonium salts with organostannanes—a new, mild and rapid synthesis of aryl [11C]ketones. J Chem Soc, Perkin Trans 1. 2000;(6):1033–6.

  5. Altomonte S, Telu S, Lu S, Pike VW. Pd(0)-Mediated 11C-carbonylation of aryl(mesityl)iodonium salts as a route to [11C]arylcarboxylic acids and derivatives. J Org Chem. 2017;82(22):11925–32.

  6. Anders DA, Bongarzone S, Fortt R, Gee AD, Long NJ. Electrochemical [11C]CO2 to [11C]CO conversion for PET imaging. ChemComm. 2017;53(20):2982–5.

  7. Andersen TL, Friis SD, Audrain H, Nordeman P, Antoni G, Skrydstrup T. Efficient 11C-carbonylation of isolated aryl palladium complexes for PET: application to challenging radiopharmaceutical synthesis. J Am Chem Soc. 2015;137(4):1548–55.

  8. Andersson Y, Långström B. Synthesis of 11C-labelled ketones via carbonylative coupling reactions using [11C]carbon monoxide. J Chem Soc, Perkin Trans 1. 1995;(4):287–9.

  9. Antoni G. Development of carbon-11 labelled PET tracers—radiochemical and technological challenges in a historic perspective. J Label Compd Radiopharm. 2015;58(3):65–72.

  10. Audrain H, Martarello L, Gee A, Bender D. Utilisation of [11C]-labelled boron carbonyl complexes in palladium carbonylation reaction. ChemComm. 2004;5:558–9.

  11. Barletta J, Karimi F, Doi H, Långström B. Synthesis of diethyl [carbonyl-11C]malonate from [11C]carbon monoxide by rhodium-promoted carbonylation and its application as a reaction intermediate. J Label Compd Radiopharm. 2006;49(9):801–9.

  12. Bergman S, Estrada S, Hall H, Rahman R, Blomgren A, Larhed M, et al. Synthesis and labeling of a piperazine-based library of 11C-labeled ligands for imaging of the vesicular acetylcholine transporter. J Label Compd Radiopharm. 2014;57(8):525–32.

  13. Bongarzone S, Taddei C, Gee AD. Poster presentation: “Optimisation of the [11C]CO2 to [11C]CO conversion mediated by 11C-labelled silane derivatives”. J Label Compd Radiopharm. 2017;60(S1):S575.

  14. Brennführer A, Neumann H, Beller M. Palladium-catalyzed carbonylation reactions of aryl halides and related compounds. Angew Chem Int Ed. 2009;48(23):4114–33.

  15. Brook AG. Thermal rearrangements of organosilicon and organogermanium compounds1. J Am Chem Soc. 1955;77(18):4827–9.

  16. Brook AG, Gilman H. Base-catalyzed elimination reactions of triphenylsilanecarboxylic acid and its derivatives. J Am Chem Soc. 1955;77(8):2322–5.

  17. Chow SY, Odell LR, Eriksson J. Low-pressure radical 11C-aminocarbonylation of alkyl iodides through thermal initiation. Eur J Org Chem. 2016;2016(36):5980–9.

  18. Clark JC, Buckingham PD. The preparation and storage of carbon-11 labelled gases for clinical use. Int J Appl Radiat Isot. 1971;22(11):639–46.

  19. Cornilleau T, Audrain H, Guillemet A, Hermange P, Fouquet E. General last-step labeling of biomolecule-based substrates by [12C], [13C], and [11C] carbon monoxide. Org Lett. 2015;17(2):354–7.

  20. Dahl K, Halldin C, Schou M. New methodologies for the preparation of carbon-11 labeled radiopharmaceuticals. Clin Transl Imaging. 2017a;5(3):275–89.

  21. Dahl K, Itsenko O, Rahman O, Ulin J, Sjöberg C-O, Sandblom P, et al. An evaluation of a high-pressure 11CO carbonylation apparatus. J Label Compd Radiopharm. 2015a;58(5):220–5.

  22. Dahl K, Nakao R, Amini N, Moein MM, Finnema S, Malmquist J, et al. Development of [carbonyl-11C]AZ13198083, a novel histamine type-3 receptor radioligand with favorable kinetics. ACS Chem Neurosci. 2018;9(5):906–11.

  23. Dahl K, Nordeman P. 11C-Carbonylation through in situ generated 11C-benzoyl chlorides with tetrabutylammonium chloride as chloride source. Eur J Org Chem. 2017;2017(18):2648–51.

  24. Dahl K, Schou M, Amini N, Halldin C. Palladium-mediated [11C]carbonylation at atmospheric pressure: a general method using Xantphos as supporting ligand. Eur J Org Chem. 2013;2013(7):1228–31.

  25. Dahl K, Schou M, Rahman O, Halldin C. Improved yields for the palladium-mediated 11C-carbonylation reaction using microwave technology. Eur J Org Chem. 2014;2014(2):307–10.

  26. Dahl K, Schou M, Ulin J, Sjöberg C-O, Farde L, Halldin C. 11C-Carbonylation reactions using gas–liquid segmented microfluidics. RSC Adv. 2015b;5(108):88886–9.

  27. Dahl K, Ulin J, Schou M, Halldin C. Reduction of [11C]CO2 to [11C]CO using solid supported zinc. J Label Compd Radiopharm. 2017b;60(13):624–8.

  28. DiFilippo A, Murali D, Ellison P, Barnhart T, Engle J, Christian B. Improved synthesis of [11C]UCB-J for PET imaging of synaptic density. J Nucl Med. 2019;60(supplement 1):1624.

  29. Donohue SR, Halldin C, Schou M, Hong J, Phebus L, Chernet E, et al. Radiolabeling of a high potency cannabinoid subtype-1 receptor ligand, N-(4-fluoro-benzyl)-4-(3-(piperidin-1-yl)-indole-1-sulfonyl)benzamide (PipISB), with carbon-11 or fluorine-18. J Label Compd Radiopharm. 2008;51(3):146–52.

  30. Dupont A-C, Largeau B, Santiago Ribeiro MJ, Guilloteau D, Tronel C, Arlicot N. Translocator protein-18 kDa (TSPO) positron emission tomography (PET) imaging and its clinical impact in neurodegenerative diseases. Int J Mol Sci. 2017;18(4):785.

  31. Eriksson J, Åberg O, Långström B. Synthesis of [11C]/[13C]acrylamides by palladium-mediated carbonylation. Eur J Org Chem. 2007;2007(3):455–61.

  32. Eriksson J, Antoni G, Långström B. Synthesis of [1-11C]ethyl iodide from [11C]carbon monoxide and its application in alkylation reactions. J Label Compd Radiopharm. 2004;47(11):723–31.

  33. Eriksson J, Antoni G, Långström B. Synthesis of [1-11C]propyl and [1-11C]butyl iodide from [11C]carbon monoxide and their use in alkylation reactions. J Label Compd Radiopharm. 2006;49(12):1105–16.

  34. Eriksson J, Hoek J, Windhorst AD. Transition metal mediated synthesis using [11C]CO at low pressure – a simplified method for 11C-carbonylation. J Label Compd Radiopharm. 2012;55(6):223–8.

  35. Fenalti G, Law RHP, Buckle AM, Langendorf C, Tuck K, Rosado CJ, et al. GABA Production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop. Nat Struct Mol Biol. 2007;14:280–6.

  36. Filp U, Pees AL, Taddei C, Pekošak A, Gee AD, Windhorst AD, et al. Efficient synthesis of 11C-acrylesters, 11C-acrylamides and their application in Michael addition reactions for PET tracer development. Eur J Org Chem. 2017;2017(34):5154–62.

  37. Finnema SJ, Donohue SR, Zoghbi SS, Brown AK, Gulyás B, Innis RB, et al. Evaluation of [11C]PipISB and [18F]PipISB in monkey as candidate radioligands for imaging brain cannabinoid type-1 receptors in vivo. Synapse. 2009;63(1):22–30.

  38. Fisher MJ, McMurray L, Lu S, Morse CL, Liow J-S, Zoghbi SS, et al. [carboxyl–11C]-Labeling of four high-affinity cPLA2α inhibitors and their evaluation as radioligands in mice with positron emission tomography. ChemMedChem. 2018;13(2):138–46.

  39. Friis SD, Taaning RH, Lindhardt AT, Skrydstrup T. Silacarboxylic acids as efficient carbon monoxide releasing molecules: synthesis and application in palladium-catalyzed carbonylation reactions. J Am Chem Soc. 2011;133(45):18114–7.

  40. Gadge ST, Bhanage BM. Recent developments in palladium catalysed carbonylation reactions. RSC Adv. 2014;4(20):10367–89.

  41. Gibanel F, López MC, Royo FM, Santafé J, Urieta JS. Solubility of nonpolar gases in tetrahydrofuran at 0 to 30°C and 101.33 kPa partial pressure of gas. J Solut Chem. 1993;22(3):211–7.

  42. Gómez-Vallejo V, Gaja V, Koziorowski J, Llop J. Specific activity of 11C-labelled radiotracers: A big challenge for PET chemists. In: Hsieh C-H, editor. Positron emission tomography - current clinical and research aspects: InTech; 2012. p. 183–209.

  43. Haskali MB, Pike VW. [11C]Fluoroform, a breakthrough for versatile labeling of PET radiotracer trifluoromethyl groups in high molar activity. Chem Eur J. 2017;23(34):8156–60.

  44. Haywood T, Cesarec S, Kealey S, Plisson C, Miller PW. Ammonium [11C]thiocyanate: revised preparation and reactivity studies of a versatile nucleophile for carbon-11 radiolabelling. MedChemComm. 2018;9(8):1311–4.

  45. Haywood T, Kealey S, Sánchez-Cabezas S, Hall JJ, Allott L, Smith G, et al. Carbon-11 radiolabelling of organosulfur compounds: 11C synthesis of the progesterone receptor agonist Tanaproget. Chem Eur J. 2015;21(25):9034–8.

  46. Hong J, Lu S, Xu R, Liow J-S, Woock AE, Jenko KJ, et al. [carbonyl-11C]4-Fluoro-N-methyl-N-(4-(6-(methylamino)pyrimidin-4-yl)thiazol-2-yl)benzamide ([11C]FIMX) is an effective radioligand for PET imaging of metabotropic glutamate receptor 1 (mGluR1) in monkey brain. Nucl Med Biol. 2015;42(12):967–74.

  47. Hostetler ED, Burns HD. A remote-controlled high pressure reactor for radiotracer synthesis with [11C]carbon monoxide. Nucl Med Biol. 2002;29(8):845–8.

  48. Ishii H, Minegishi K, Nagatsu K, Zhang M-R. Pd(0)-Mediated [11C]carbonylation of aryl and heteroaryl boronic acid pinacol esters with [11C]carbon monoxide under ambient conditions and a facile process for the conversion of [carbonyl-11C]esters to [carbonyl-11C]amides. Tetrahedron. 2015;71(10):1588–96.

  49. Ito H, Okubo Y, Halldin C, Farde L. Mapping of central D2 dopamine receptors in man using [11C]raclopride: PET with anatomic standardization technique. NeuroImage. 1999;9(2):235–42.

  50. Itsenko O, Blom E, Långström B, Kihlberg T. The use of lithium amides in the palladium-mediated synthesis of [carbonyl-11C]amides. Eur J Org Chem. 2007;2007(26):4337–42.

  51. Itsenko O, Kihlberg T, Långström B. Photoinitiated carbonylation with [11C]carbon monoxide using amines and alkyl iodides. J Org Chem. 2004;69(13):4356–60.

  52. Kealey S, Gee A, Miller PW. Transition metal mediated [11C]carbonylation reactions: recent advances and applications. J Label Compd Radiopharm. 2014b;57(4):195–201.

  53. Kealey S, Miller PW, Long NJ, Plisson C, Martarello L, Gee AD. Copper(I) scorpionate complexes and their application in palladium-mediated [11C]carbonylation reactions. ChemComm. 2009;25:3696–8.

  54. Kealey S, Plisson C, Collier TL, Long NJ, Husbands SM, Martarello L, et al. Microfluidic reactions using [11C]carbon monoxide solutions for the synthesis of a positron emission tomography radiotracer. Org Biomol Chem. 2011;9(9):3313–9.

  55. Kealey S, White AJP, Gee AD, Long NJ. Evaluation of [12C/11C]carbon monoxide binding to copper(I) tris(pyrazolyl)borate complexes. Eur J Inorg Chem. 2014a;2014(11):1896–905.

  56. Kihlberg T, Bengt L, Tommy F, Jonas E. Methods and apparatus for production and use of [11C]carbon monoxide in labeling synthesis. 2006. patent WO 2006/008603.

  57. Kihlberg T, Karimi F, Långström B. [11C]Carbon monoxide in selenium-mediated synthesis of 11C-carbamoyl compounds. J Org Chem. 2002;67(11):3687–92.

  58. Kihlberg T, Långström B. Biologically active 11C-labeled amides using palladium-mediated reactions with aryl halides and [11C]carbon monoxide. J Org Chem. 1999;64(25):9201–5.

  59. Krasikova RN, Andersson J, Truong P, Nag S, Shchukin EV, Halldin C. A fully automated one-pot synthesis of [carbonyl-11C]WAY-100635 for clinical PET applications. Appl Radiat Isot. 2009;67(1):73–8.

  60. Landais P, Finn R. On-line preparation of [11C]carbon dioxide from [11C]methane. Int J Rad Appl Instrum A. 1989;40(3):265–6.

  61. Långström B, Itsenko O, Rahman O. [11C]Carbon monoxide, a versatile and useful precursor in labelling chemistry for PET-ligand development. J Label Compd Radiopharm. 2007;50(9–10):794–810.

  62. Lescot C, Nielsen DU, Makarov IS, Lindhardt AT, Daasbjerg K, Skrydstrup T. Efficient fluoride-catalyzed conversion of CO2 to CO at room temperature. J Am Chem Soc. 2014;136(16):6142–7.

  63. Lidström P, Kihlberg T, Långström B. [11C]Carbon monoxide in the palladium-mediated synthesis of 11C-labelled ketones. J Chem Soc, Perkin Trans 1. 1997;(18):2701–6.

  64. Lindberg A, Lu S, Nag S, Schou M, Liow J-S, Zoghbi SS, et al. Evaluation of agonist 5-HT1B PET radioligands. Nucl Med Biol. 2019;70:1–13.

  65. Lindhe Ö, Almqvist P, Kågedal M, Gustafsson S-Å, Bergström M, Nilsson D, et al. Autoradiographic mapping of binding sites in the rhesus monkey brain using [carbonyl-11C]zolmitriptan. Int J Mol Imaging. 2011;2011:6.

  66. Lohith T, Fabrice S, Zoghbi S, Morse C, Pike V, Innis R, et al. Potential utility of 11C-FPEB as a PET ligand for imaging metabotropic glutamate receptor 5 (mGluR5) in human brain. J Nucl Med. 2014;55(supplement 1):360.

  67. Lohith TG, Tsujikawa T, Siméon FG, Veronese M, Zoghbi SS, Lyoo CH, et al. Comparison of two PET radioligands, [11C]FPEB and [11C]SP203, for quantification of metabotropic glutamate receptor 5 in human brain. J Cereb Blood Flow Metab. 2017;37(7):2458–70.

  68. Lu S, Zhang Y, Kalin J, Cai L, Kozikowski AP, Pike VW. Exploration of the labeling of [11C]tubastatin A at the hydroxamic acid site with [11C]carbon monoxide. J Label Compd Radiopharm. 2016;59(1):9–13.

  69. Miller PW, Audrain H, Bender D, deMello AJ, Gee AD, Long NJ, et al. Rapid carbon-11 radiolabelling for PET using microfluidics. Chem Eur J. 2011;17(2):460–3.

  70. Miller PW, Long NJ, Vilar R, Gee AD. Synthesis of 11C, 18F, 15O, and 13N radiolabels for positron emission tomography. Angew Chem Int Ed. 2008;47(47):8998–9033.

  71. Nabulsi NB, Mercier J, Holden D, Carré S, Najafzadeh S, Vandergeten M-C, et al. Synthesis and preclinical evaluation of 11C-UCB-J as a PET tracer for imaging the synaptic vesicle glycoprotein 2A in the brain. J Nucl Med. 2016;57(5):777–84.

  72. Nielsen DU, Neumann KT, Lindhardt AT, Skrydstrup T. Recent developments in carbonylation chemistry using [13C]CO, [11C]CO, and [14C]CO. J Label Compd Radiopharm. 2018;61:949–87.

  73. Noguchi J, Suzuki K. Automated synthesis of the ultra high specific activity of [11C]Ro15-4513 and its application in an extremely low concentration region to an ARG study. Nucl Med Biol. 2003;30(3):335–43.

  74. Nordeman P, Estrada S, Odell LR, Larhed M, Antoni G. 11C-Labeling of a potent hydroxyethylamine BACE-1 inhibitor and evaluation in vitro and in vivo. Nucl Med Biol. 2014;41(6):536–43.

  75. Nordeman P, Friis SD, Andersen TL, Audrain H, Larhed M, Skrydstrup T, et al. Rapid and efficient conversion of 11CO2 to 11CO through silacarboxylic acids: applications in Pd-mediated carbonylations. Chem Eur J. 2015;21(49):17601–4.

  76. Okubo Y, Olsson H, Ito H, Lofti M, Suhara T, Halldin C, et al. PET mapping of extrastriatal D2-like dopamine receptors in the human brain using an anatomic standardization technique and [11C]FLB 457. NeuroImage. 1999;10(6):666–74.

  77. Pike VW. The status of PET radiochemistry for drug development and evaluation. Drug Inf J. 1997;31(3):997–1013.

  78. Pike VW. Hypervalent aryliodine compounds as precursors for radiofluorination. J Label Compd Radiopharm. 2018;61(3):196–227.

  79. Pike VW, Eakins MN, Allan RM, Selwyn AP. Preparation of [1−11C]acetate—an agent for the study of myocardial metabolism by positron emission tomography. Appl Radiat Isot. 1982;33(7):505–12.

  80. Poot AJ, van der Wildt B, Stigter-van Walsum M, Rongen M, Schuit RC, Hendrikse NH, et al. [11C]Sorafenib: radiosynthesis and preclinical evaluation in tumor-bearing mice of a new TKI-PET tracer. Nucl Med Biol. 2013;40(4):488–97.

  81. Rahman O. [11C]Carbon monoxide in labeling chemistry and positron emission tomography tracer development: scope and limitations. J Label Compd Radiopharm. 2015;58(3):86–98.

  82. Rahman O, Långström B. Synthesis of N-(2,5-dimethoxybenzyl)-N-(5-fluoro-2-phenoxyphenyl)[carbonyl-11C]acetamide ([carbonyl-11C]DAA1106) and analogues using [11C]carbon monoxide and palladium(0) complex. J Label Compd Radiopharm. 2007;50(13):1192–9.

  83. Rahman O, Långström B, Halldin C. Alkyl iodides and [11C]CO in nickel-mediated cross-coupling reactions: successful use of alkyl electrophiles containing a β hydrogen atom in metal-mediated [11C]carbonylation. ChemistrySelect. 2016;1(10):2498–501.

  84. Rahman O, Takano A, Amini N, Dahl K, Kanegawa N, Långström B, et al. Synthesis of ([11C]carbonyl)raclopride and a comparison with ([11C]methyl)raclopride in a monkey PET study. Nucl Med Biol. 2015;42(11):893–8.

  85. Roeda D, Crouzel C, Dollé F. A rapid, almost quantitative conversion of [11C]carbon dioxide into [11C]carbon monoxide via [11C]formate and [11C]formyl chloride. Radiochimica Acta. 2004;92:329–32.

  86. Roeda D, Dollé F. [11C]Phosgene: a versatile reagent for radioactive carbonyl insertion into medicinal radiotracers for positron emission tomography. Curr Top Med Chem. 2010;10(16):1680–700.

  87. Roslin S, Brandt P, Nordeman P, Larhed M, Odell LR, Eriksson J. Synthesis of 11C-labelled ureas by palladium(II)-mediated oxidative carbonylation. Molecules. 2017;22(10):1688.

  88. Roslin S, Dahl K, Nordeman P. Reaction of 11C-benzoyl chlorides with metalloid reagents: 11C-labeling of benzyl alcohols, benzaldehydes, and phenyl ketones from [11C]CO. J Label Compd Radiopharm. 2018;61(5):447–54.

  89. Rotstein BH, Liang SH, Holland JP, Collier TL, Hooker JM, Wilson AA, et al. 11CO2 Fixation: a renaissance in PET radiochemistry. ChemComm. 2013;49(50):5621–9.

  90. Rotstein BH, Liang SH, Placzek MS, Hooker JM, Gee AD, Dollé F, et al. 11C=O Bonds made easily for positron emission tomography radiopharmaceuticals. Chem Soc Rev. 2016;45(17):4708–26.

  91. Sasaki T, Ogawa K, Ishii S-I, Senda M. Synthesis of [11C]salicylic acid and related compounds and their biodistribution in mice. Appl Radiat Isot. 1999;50(5):905–9.

  92. Scott PJH. Methods for the incorporation of carbon-11 to generate radiopharmaceuticals for PET imaging. Angew Chem Int Ed. 2009;48(33):6001–4.

  93. Shen HC, Hammock BD. Discovery of inhibitors of soluble epoxide hydrolase: a target with multiple potential therapeutic indications. J Med Chem. 2012;55(5):1789–808.

  94. Siméon GF, Culligan JW, Lu S, Pike WV. 11C-Labeling of aryl ketones as candidate histamine subtype-3 receptor PET radioligands through Pd(0)-mediated 11C-carbonylative coupling. Molecules. 2017;22(5).

  95. Slobbe P, Windhorst AD, Adamzek K, Bolijn M, Schuit RC, Heideman DAM, et al. Development of [11C]vemurafenib employing a carbon-11 carbonylative Stille coupling and preliminary evaluation in mice bearing melanoma tumor xenografts. Oncotarget. 2017;8(24):38337–50.

  96. Stevens MY, Chow SY, Estrada S, Eriksson J, Asplund V, Orlova A, et al. Synthesis of 11C-labeled sulfonyl carbamates through a multicomponent reaction employing sulfonyl azides, alcohols, and [11C]CO. ChemistryOpen. 2016;5(6):566–73.

  97. Taddei C, Bongarzone S, Gee AD. Instantaneous conversion of [11C]CO2 to [11C]CO via fluoride-activated disilane species. Chem Eur J. 2017a;23(32):7682–5.

  98. Taddei C, Bongarzone S, Haji Dheere AK, Gee AD. [11C]CO2 to [11C]CO conversion mediated by [11C]silanes: a novel route for [11C]carbonylation reactions. ChemComm. 2015;51(59):11795–7.

  99. Taddei C, Filp U, Pekošak A, Poot AJ, Windhorst AD, Gee AD. Poster presentation: "Synthesis and purification of a novel 11C-labelled GAD inhibitor". J Label Compd Radiopharm. 2017b;60(S1):S208

  100. Taddei C, Gee AD. Recent progress in [11C]carbon dioxide ([11C]CO2) and [11C]carbon monoxide ([11C]CO) chemistry. J Label Compd Radiopharm. 2018;61(3):237–51.

  101. Telu S, Siméon FG, Lu S, Pike VW. Hypervalent iodine compounds as precursors for biomedical radiotracers. In: Marek I, Olofson B, Rappoport Z, editors. The chemistry of hypervalent halogen compounds, PATAI'S chemistry of functional groups. Chichester: Wiley; 2019. p. 721–80.

  102. Terry GE, Hirvonen J, Liow J-S, Zoghbi SS, Gladding R, Tauscher JT, et al. Imaging and quantitation of cannabinoid CB1 receptors in human and monkey brains using 18F-labeled inverse agonist radioligands. J Nucl Med. 2010;51(1):112–20.

  103. Terry GE, Liow J-S, Zoghbi SS, Hirvonen J, Farris AG, Lerner A, et al. Quantitation of cannabinoid CB1 receptors in healthy human brain using positron emission tomography and an inverse agonist radioligand. NeuroImage. 2009;48(2):362–70.

  104. USP. Chemical Tests / <232> Elemental Impurities—Limits. USP 40­-NF 35, First Supplement; 2017. p. 8065–9.

  105. van der Wildt B, Wilhelmus MMM, Bijkerk J, Haveman LYF, Kooijman EJM, Schuit RC, et al. Development of carbon-11 labeled acryl amides for selective PET imaging of active tissue transglutaminase. Nucl Med Biol. 2016;43(4):232–42.

  106. Verbeek J, Eriksson J, Syvänen S, Labots M, de ECM L, Voskuyl RA, et al. [11C]Phenytoin revisited: synthesis by [11C]CO carbonylation and first evaluation as a P-gp tracer in rats. EJNMMI Res. 2012;2(1):36.

  107. Wey H-Y, Gilbert TM, Zürcher NR, She A, Bhanot A, Taillon BD, et al. Insights into neuroepigenetics through human histone deacetylase PET imaging. Sci Transl Med. 2016;8(351):351ra106.

  108. Xu R, Zanotti-Fregonara P, Zoghbi SS, Gladding RL, Woock AE, Innis RB, et al. Synthesis and evaluation in monkey of [18F]4-fluoro-N-methyl-N-(4-(6-(methylamino)pyrimidin-4-yl)thiazol-2-yl)benzamide ([18F]FIMX): a promising radioligand for PET imaging of brain metabotropic glutamate receptor 1 (mGluR1). J Med Chem. 2013;56(22):9146–55.

  109. Zeisler SK, Nader M, Theobald A, Oberdorfer F. Conversion of no-carrier-added [11C]carbon dioxide to [11C]carbon monoxide on molybdenum for the synthesis of 11C-labelled aromatic ketones. Appl Radiat Isot. 1997;48(8):1091–5.

  110. Zhang M-R, Kida T, Noguchi J, Furutsuka K, Maeda J, Suhara T, et al. [11C]DAA1106: Radiosynthesis and in vivo binding to peripheral benzodiazepine receptors in mouse brain. Nucl Med Biol. 2003;30(5):513–9.

  111. Zhang M-R, Suzuki K. Sources of carbon which decrease the specific activity of [11C]CH3I synthesized by the single pass I2 method. Appl Radiat Isot. 2005;62(3):447–50.

Download references

Acknowledgements

The authors would like to acknowledge Dr. Shuiyu Lu for productively proof-reading this review before submission. In addition, the authors thank the reviewers for their constructive comments on the manuscript. Their suggestions have surely helped to improve this review.

Funding

The authors were supported by the Intramural Research Program of NIH (National Institute of Mental Health; project number ZIA-MH002793).

Author information

CT developed the review outline, drew the figures and produced the main structure of the manuscript. VWP productively collaborated providing additional reference material and suggestions in the manuscript content. Both authors approved the final manuscript.

Correspondence to Carlotta Taddei.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Both authors gave their consent for publication.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Taddei, C., Pike, V.W. [11C]Carbon monoxide: advances in production and application to PET radiotracer development over the past 15 years. EJNMMI radiopharm. chem. 4, 25 (2019) doi:10.1186/s41181-019-0073-4

Download citation

Keywords

  • Carbon-11
  • PET
  • Carbon monoxide
  • Radiochemistry
  • Carbonylation
  • Radiotracer