Fig. 5From: Improved automated one-pot two-step radiosynthesis of (S)-[18F]FETrp, a radiotracer for PET imaging of indoleamine 2,3-dioxygenase 1 (IDO1)Representative HPLC chromatograms of formulated (S)-[18F]FETrp. Analytical HPLC chromatograms of (S)-[18F]FETrp (B) (radioactivity) co-injected with (S)-FETrp (A) (UV, λ at 290 nm) on Agilent Zorbax Extend C18 column. Chiral HPLC chromatograms of (S)-[18F]FETrp (D) (radioactivity) co-injected with (S/R)-FETrp (C) (UV, λ at 290 nm) on Reprosil Chiral-AA column. The radio-HPLC detectors were connected in series after the UV detectors accounting for the slight differences in retention times (∼0.3 min) observed between UV and radioactive signalsBack to article page