Table 2 Cassette position and materials/reagents list used in the radiosynthesis of [18F]FSPG via FASTlab™ module
From: Simplified and highly-reliable automated production of [18F]FSPG for clinical studies
aCassette position (CP) | Reagents or consumables |
|---|---|
1 | Short tubing to 18O water collection vial |
2 | Potassium carbonate/kryptofix QMA elution solution, (11 mm Vial, 0.8 mL) |
3 | Left hand syringe (LHS), syringe 1 |
4 | QMA light SepPak cartridge, 1 pre-condition cartridge |
5 | Short tubing to QMA light SepPak cartridge at CP4 |
6 | 18F Inlet |
7 | Short tubing to reactor side port |
8 | Short tubing to reactor center port |
9 | 1M H2SO4, long tubing connected to external 4.0 mL 1M H2SO4 vial |
10 | bPhosphate buffered saline (PBS), long tubing connected to external 20 mL PBS vial |
11 | Middle syringe (MS), syringe 2 |
12 | FSPG precursor, (11 mm Vial, 6 -12 mg dissolved in 1.3 mL anhydrous acetonitrile) |
13 | Unused |
14 | 1M H2SO4, (13 mm Vial, 2.2 mL) |
15 | Water Spike/water bag, 100 mL SWI bag |
16 | 4M NaOH (13 mm Vial, 1.7 mL) |
17 | Short tubing to Oasis MCX cartridge at CP18 |
18 | Oasis® MCX cartridge, 1 cartridge |
19 | Short tubing to Oasis MCX cartridge at CP20 |
20 | Oasis® MCX cartridge, 1 cartridge |
21 | Short tubing to Alumina N plus long SepPak cartridge at CP22 |
22 | Alumina N plus long SepPak cartridge, 1 cartridge |
23 | Connecting tube to Superclean™ ENVI-Carb™ packed column, 1 set |
24 | Right hand syringe (RHS), syringe 3 |
25 | Long tubing to reactor side port |
26 | Final Product Vial, 1 vial |