Simplified and highly-reliable automated production of [18F]FSPG for clinical studies

EJNMMI Radiopharmacy and Chemistry

Table 1 Material and reagent list used in the radiosynthesis of [¹⁸F]FSPG via TRACERlab™ FXFN module

^aItem #	Reagents or consumables
1	Potassium carbonate/kryptofix QMA elution solution, 0.6 mL
2	Anhydrous acetonitrile, 0.6 mL
3	FSPG precursor, 6 mg dissolved in 1.0 mL anhydrous acetonitrile
4	1M H₂SO₄, 2.0 mL
5	4M NaOH, 1.5 mL
6	1M H₂SO₄, 4.0 mL
7	Sterile water for injection (SWI), 15.0 mL
8	^bPhosphate buffer solution, 20.0 mL
9	Pre-conditioned QMA light SepPak cartridge, 1 cartridge
10	^cOasis^® MCX cartridge/glass membrane filter assembly, 1 set
11	^dAlumina N plus long cartridge/Superclean™ ENVI-Carb™ assembly, 1 set
12	Final Product vial, 1 vial

^aItems designation and position are indicated in Fig. 3
^bFormulation of phosphate buffer solution is specified in the Materials and methods “General” section
^cIllustration of the Oasis^® MCX cartridge/glass membrane filter assembly is shown in Fig. 5a
^dIllustration of the Alumina N Cartridge/Superclean™ ENVI-Carb™ assembly is shown in Fig. 5b