Table 1 Summary of the radiolabeling conditions
| Â | Condition 1 | Condition 2 | Condition 3 |
|---|---|---|---|
MilliQ water |  | 10 μL | 10 μL |
MilliQ water containing Kolliphor® HS 15 (2.0 mg/mL) | 90.5 μL |  |  |
TRIS buffer containing the peptide (0.1 M, pH 9) |  | 75 μL | 75 μL |
Ascorbate buffer (1.0 M, pH 5.8) |  | 50 μL | 50 μL |
l-melatonin (0.5 M) |  |  | 10 μL |
Gentisic/ascorbic acids (50 mM) | 10 μL |  |  |
Sodium acetate (2.5 M, pH 8) | 1 μL |  |  |
Ethanol | 10 μL |  |  |
Molar activity | 50Â kBq/nmol | 100Â kBq/nmol | 100Â kBq/nmol |
DTPA (4 mM) | 5 μL | 5 μL | 5 μL |