Table 1 Binding affinity kinetics (equilibrium dissociation constant (KD), association (ka) and dissociation (kd) rates) of anti-CD70 VHHs as determined by SPR
From: Site-specific 68Ga-labeled nanobody for PET imaging of CD70 expression in preclinical tumor models
Ligand | Analyte | ka (1/M.s) | kd (1/s) | KD (nM) |
|---|---|---|---|---|
Anti-CD70 VHH C-Direct tag | Human CD70 | 1.16 × 106 | 1.18 × 10–3 | 1.020 |
Anti-CD70 VHH C-Direct tag | Mouse CD70 | No binding | ||
NOTA-anti-CD70 VHH | Human CD70 | 7.33 × 105 | 1.23 × 10–4 | 0.208 |
Anti-CD70 VHH biotin-tag | Human CD70 | 8.38 × 105 | 1.22 × 10–4 | 0.145 |