Fig. 1

In vitro characterization of [⁶⁸Ga]Ga-NOTA-anti-CD70 VHH. A Analysis of CD70 protein expression measured by flow cytometry using in the upper trace anti-CD70 VHH-Hilyte Fluor 488 (107B8) or control VHH-Hilyte Fluor 488 and in the lower trace PE mouse anti-human CD70 or PE mouse IgG3 isotype control. B Relative amount of cell associated activity of [⁶⁸Ga]Ga-NOTA-anti-CD70 VHH (5 nM, 0.04 µg) on CD70^high and CD70^low cells (1 × 10⁶), or in the presence of excess unlabeled VHH (50 µg, block). (C) Specific binding of [⁶⁸Ga]Ga-NOTA-anti-CD70 VHH on Raji cells (5 × 10⁵). (****p < 0.0001)