Table 2 Starting materials used in in-house prepared and original cassettes for [18F]FMISO FASTlab
Position | In-house prepared cassette | Original cassette |
|---|---|---|
1 | Unaltered | |
2 | Eluent, (acetonitrile/150 mM N(Bu)4HCO3 75:25), (1.0 mL) | Mixture (0.8 mL) |
3 | Unaltered | |
4 | SepPak, QMA light cartridge, carbonate form, 130 mg | SepPak, QMA light cartridge, carbonate form |
5 | Unaltered | |
7–8 | Unaltered | |
11 | Unaltered | |
12 | Precursor (NITTP, 10 mg), dissolved in acetonitrile (0.2 mL) | Precursor (NITTP, 7 mg), powder |
13 | Acetonitrile (4.0 mL) | Acetonitrile (4.2 mL) |
14 | Phosphoric acid 2.3 M (4.0 mL) | Phosphoric acid (4.2 mL) |
15 | Unaltered | |
16 | Ethanol 95% (4,0 mL) | Ethanol (4,0 mL) |
17 | Unaltered | |
18 | Oasis HLB cartridge (225 mg) | Oasis HLB cartridge |
19 | Unaltered | |
20 | SepPak Alumina B cartridge (280 mg) | SepPak Alumina A cartridge |