Table 1 Flow gradient used to perform HPLC analysis
HPLC analysis (flow = 1 mL/min) | ||
|---|---|---|
Time (min) | Mobile phase A (%) Water/TFA 1000/1 v/v | Mobile phase B (%) Acetonitrile/TFA 1000/1 v/v |
0 | 80 | 20 |
9 | 50 | 50 |
10 | 50 | 50 |
15 | 80 | 20 |
HPLC analysis (flow = 1 mL/min) | ||
|---|---|---|
Time (min) | Mobile phase A (%) Water/TFA 1000/1 v/v | Mobile phase B (%) Acetonitrile/TFA 1000/1 v/v |
0 | 80 | 20 |
9 | 50 | 50 |
10 | 50 | 50 |
15 | 80 | 20 |