Fig. 3

Metabolic stability and arterial blood clearance of [¹⁸F]7b in a normal Wistar rat. A Radio-HPLC chromatograms of [¹⁸F]7b in samples of blood and excretion media taken at different time points after i.v. injection of [¹⁸F]7b. B Time course of arterial blood clearance of intact [¹⁸F]7b as calculated from activity measurement of blood samples and fraction of intact [¹⁸F]7b determined by radio-HPLC and radio-TLC (Additional file 1: Fig. S3) including nonlinear regression according to two-phase decay. The inset shows the respective semi-logarithmic plot with separate linear regressions for the time points 1–3 min (dotted line, extrapolated up to the x-axis) and 3–30 min (solid line, extrapolated up to the y-axis). From the results of the latter linear regression, c₀₂ and k_el were derived (see Methods section). Data in A and B are from the same experiment, in which [¹⁸F]7b (69.8 MBq, 5.27 nmol) was injected into a healthy Wistar rat (body weight of 170 g)