Table 5 Summary of pros and cons of mixing techniques
Mixing technique | Pros | Cons |
|---|---|---|
Normal flow | Simple setup and procedure | Poor mixing due to bubble formation |
Easy to implement | Poor or no radiolabeling achieved | |
Continuous flow | Good radiolabeling in some cases | High back pressure on R1 affects mixing |
Continuous mixing of reagents in R2 | Reproducibility issue with various batches of cassette | |
Alternative flow | Average to good radiolabeling yield | Poor mixing and bubble formation in R2 |
Partial elution and addition of buffer mixture | Issues with filling R2 | |
Pulse flow | Achieved reproducibility and high radiolabeling yield | Requires precise timing for opening MFVs |
Increased surface area for reagent interaction | Careful control needed to avoid bubble formation |