Trapping and elution | Mixing technique | No of synthesis (n) | Eluent (µL) | Buffer volume (µL) | Peptide (µg) | Radiochemical yielddc (%)** |
---|---|---|---|---|---|---|
Direct trapping | Normal flow | 8 | 170* | 110 | 20 | 64 ± 16 |
Reverse trapping | Normal flow | 12 | 170 | 110 | 20 | 27 ± 28 |
Reverse trapping | Continuous flow | 16 | 140 | 120 | 20 | 67 ± 16 |
Reverse trapping | Alternative flow | 6 | 120 | 160 | 20 | 68 ± 18 |
Reverse trapping | Pulse flow | 4 | 200 | 150 | 20 | 79 ± 8 |