Table 3 Analytical HPLC method instrument setup
From: Optimized, automated and cGMP-compliant synthesis of the HER2 targeting [68Ga]Ga-ABY-025 tracer
HPLC Agilent 1260 (Agilent/LabLogic) | Pump G7111B Manuel injector G1328C (50 µL) 6-column selection valve G1170A (optional) VWR detector GG7114A Radiodetector Flow-RAM • 1″ NaI PMT • Blue tubing 0.25 mm, wrapping 5 rounds Evaluation software Laura 6 (LabLogic) | ||
Column | Analytical column: Poroshell 120 EC-C18, 3 × 150 mm, 2.7 µm Guard Column: Poroshell 120 EC-C18 Fast guard, 3 × 5 mm, 2.7 µm | ||
Mobile phase | A: 0.1% trifluoroacetic acid in water B: acetonitrile C: 80% acetonitrile in water | ||
Gradient | Time (min) | Mobile phase composition | |
Initial | 80% A | 20% B | |
1 min 30 s | 80% A | 20% B | |
10 min | 40% A | 60% B | |
11 min | 40% A | 60% B | |
11 min 10 s | 80% A | 20% B | |
Run time | 15 min | ||
Flow Rate | 0.3 mL/min | ||
Column temperature | Room temperature | ||
Injection volume: | 50 µL | ||
Detection | UV absorbance at 220 nm | ||