Table 4 Radiosynthetic steps in the synthesis of [68Ga]FAPI-46
From: Fully automated radiosynthesis of [68Ga]Ga-FAPI-46 with cyclotron produced gallium
Step | Description | Parameter |
|---|---|---|
1 | Transfer of precursor to reactor and bombardment | 1.1 mL precursor in 1M NaOAc buffer to reactor |
2 | Delivery of [68Ga]Ga(NO3)2 to receiving vial | – |
3 | Dilute of [68Ga]Ga(NO3)2 solution to < 0.1 M HNO3 | 6 mL H2O |
4 | Trapping on ZR resin | |
5 | Washing of ZR resin | 2 × 7.3 mL 0.1 M HNO3 |
6 | Elution of ZR resin and trapping on TK resin | 5–6 mL ~ 1.75 M HCl |
7 | Washing of TK resin | 3.5 mL 2.0 M NaCl in 0.13 M HCl |
8 | Elution of TK resin to reactor | 2.7 mL H2O and dilute HCl |
9 | Radiolabeling | 95 °C for 10 min |
10 | Cooling and dilution | 2 mL 0.9% Saline |
11 | Transfer to final vial | Passing through CM cartridge and sterilization filter |
12 | Reactor wash | 4 mL 0.9% saline |
13 | Transfer to final vial | Passing through CM cartridge and sterilization filter |