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Table 1 Labeling of Dha and maleimide molecules with [18F]FPySH

From: Novel [18F]-labeled thiol for the labeling of Dha- or maleimide-containing biomolecules

Entry Substratea Conditionsb Temperature (°C) Reaction time (min) Product Conversion (%)
1 13 25 45 [18F]-16 0
2 13 TCEP, K2CO3 25 45 [18F]-16 46
3 13 TCEP, K2CO3 40 15 [18F]-16 62
4 13 TCEP, K2CO3 40 45 [18F]-16 97
5 14 TCEP, K2CO3 50 30 [18F]-17 32
6 14 TCEP, K2CO3 80 30 [18F]-17 100
7 15 TCEP, K2CO3 50 45 [18F]-18 0
8 15 TCEP, K2CO3 80 45 [18F]-18 0
9 20 TCEP, K2CO3 25 15 [18F]-21 > 95
10 20 25 15 [18F]-21 > 95
  1. aAll additions were carried out on 0.5–2.0 mg of substrate
  2. b8 mM TCEP and 8 mM K2CO3 were used for entries 2–9