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Table 2 Summary of performance of the formulation step. All % values are calculated in reference to starting pure [18F]FBB activity

From: A simple and efficient automated microvolume radiosynthesis of [18F]Florbetaben

 

Manual Formulationa

Manual Formulationa

Automated Formulation

Mass of C18 resin in cartridge (mg)

5

10

10

Number of repeats

n = 1

n = 3

n = 3

Starting pure [18F]FBB activity (MBq)

7.4

15 ± 11

112 ± 51

Formulation efficiency (%)

77

91 ± 5

81 ± 5

Formulation time (min)

18

22

17

Losses:

 Activity in waste (%)

19

1 ± 1

3 ± 3

 Fraction collection vial residual activity (%)

3

6 ± 4

2 ± 1

 Cartridge residual activity (%)

0

0 ± 1

1 ± 1

 Residual in the system (%)

N/A

N/A

14 ± 4

  1. aInitial manual formulation runs were performed using 5 nmol of FBB spiked with purified [18F]FBB product, to mimic the mass loading of a higher activity level, while working with less radioactivity