1) Azeotropic drying and complex [18F]KF/K222 formation | 1- [18F]Fluoride trapping on a QMA after production and transfer from the cyclotron |
2- Elution of [18F]Fluoride in the reactor with a solution of K222/K2CO3 | |
3- Azeotropic drying of [18F]Fluoride with successive addition of acetonitrile | |
2) [18F]radiofluorination of 4 | 1- Addition of the precursor 4 |
2- [18F]Radiofluorination | |
3) Purification of [18F]LBT999 | 1- Cooling and Dilution of the crude mixture with water or a solution of water in presence of Na-Asc (0.5%). |
2- Pre-purification on C18 and washing with water or a solution of water in presence of Na-Asc (0.5%). | |
3- Elution of crude product and injection into the HPLC loop | |
4- HPLC Purification | |
5- Collection of the product | |
6- Trapping on C18 cartridge and washing | |
4) Formulation and dispensing of [18F]LBT999 | 1- Elution with 10% EtOH and saline (0.9%) or saline (0.9%) with Na-Asc (0.5%) |
2- Transfer of formulated [18F]LBT999 and vial dispensing after sterile filtration |