Fig. 3

Elution profile of Gd, Tb, and Dy (10 mg, 1 mg, and 1 mg respectively) TK212 (a), TK211(b) and TK221 (c). Tb containing fractions were combined for loading on the next column to simulate purfication run. Column bed volumes were 1 mL for each column during cold test but were later optimized for active runs. Overall process diagram (d) note steps i–v can be performed automactically, while steps vi–viii must be performed manually