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Table 1 Labeling of Dha and maleimide molecules with [18F]FPySH

From: Novel [18F]-labeled thiol for the labeling of Dha- or maleimide-containing biomolecules

Entry

Substratea

Conditionsb

Temperature (°C)

Reaction time (min)

Product

Conversion (%)

1

13

25

45

[18F]-16

0

2

13

TCEP, K2CO3

25

45

[18F]-16

46

3

13

TCEP, K2CO3

40

15

[18F]-16

62

4

13

TCEP, K2CO3

40

45

[18F]-16

97

5

14

TCEP, K2CO3

50

30

[18F]-17

32

6

14

TCEP, K2CO3

80

30

[18F]-17

100

7

15

TCEP, K2CO3

50

45

[18F]-18

0

8

15

TCEP, K2CO3

80

45

[18F]-18

0

9

20

TCEP, K2CO3

25

15

[18F]-21

> 95

10

20

25

15

[18F]-21

> 95

  1. aAll additions were carried out on 0.5–2.0 mg of substrate
  2. b8 mM TCEP and 8 mM K2CO3 were used for entries 2–9