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Fig. 6 | EJNMMI Radiopharmacy and Chemistry

Fig. 6

From: Automated radiosynthesis of two 18F-labeled tracers containing 3-fluoro-2-hydroxypropyl moiety, [18F]FMISO and [18F]PM-PBB3, via [18F]epifluorohydrin

Fig. 6

Preparative HPLC (A) and analytical HPLC (B) chromatograms of [18F]PM-PBB3 by 18F-fluoroalkylation using [18F]5. The preparative HPLC conditions were as follows: Capcell Pak C18 column (5 μm, 10 mm i.d. × 250 mm length; Shiseido), a mixture of acetonitrile, water and triethylamine (40:60:0.1, vol./vol./vol.) as the mobile phase, 5.0 mL/min flow rate, and UV detection at 365 nm. The retention time of [18F]PM-PBB3 was 14.9 min (A). The analytical HPLC conditions were as follows: Atlantis T3 column (5 μm, 4.6 mm i.d. × 150 mm length; Waters), a mixture of acetonitrile and 50 mM ammonium acetate (40:60, vol./vol.) as the mobile phase, 1.0 mL/min flow rate, and UV detection at 365 nm. The retention time of [18F]PM-PBB3 was 12 min (B)

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