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EJNMMI Radiopharmacy and Chemistry
Fig. 5 | EJNMMI Radiopharmacy and Chemistry

Fig. 5

From: Automated radiosynthesis of two 18F-labeled tracers containing 3-fluoro-2-hydroxypropyl moiety, [18F]FMISO and [18F]PM-PBB3, via [18F]epifluorohydrin

Fig. 5

Preparative HPLC (A) and analytical HPLC (B) chromatograms of [18F]FMISO by 18F-fluoroalkylation using [18F]5. The preparative HPLC conditions were as follows: XBridge C18 column (5 μm, 10 mm i.d. × 250 mm length; Waters), a mixture of ethanol and water (2:98, vol./vol.) as the mobile phase, 5.0 mL/min flow rate, and UV detection at 325 nm. The retention time of [18F]FMISO was approximately 12 min (A). The analytical HPLC conditions were as follows: XBridge C18 column (5 μm, 4.6 mm i.d. × 150 mm length; Waters), a mixture of 90 % acetonitrile solution and 50 mM ammonium phosphate buffer (pH 9.3) (7:93, vol./vol.) as the mobile phase, 1.0 mL/min flow rate, and UV detection at 325 nm. The retention time of [18F]FMISO was 5.6 min (B)

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