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Fig. 1 | EJNMMI Radiopharmacy and Chemistry

Fig. 1

From: Improved non-invasive positron emission tomographic imaging of chemotherapy-induced tumor cell death using Zirconium-89-labeled APOMAB®

Fig. 1

Immunoreactivity and stability of conjugates and radioconjugates of chDAB4. Binding of chDAB4 to the La peptide was measured by ELISA and is equivalent for DFO-NCS-chDAB4, [89Zr]Zr-DFO-NCS-chDAB4, [89Zr]Zr-DFOSq-chDAB4, and [124I]I-labeled chDAB4 (n = 3) (a). Lineweaver-Burk plots show values for the immunoreactive fraction (IRF) determined by the Lindmo assay. The IRF values for the [89Zr]Zr-labeled DFO-conjugates of chDAB4, [89Zr]Zr-DFO-NCS and 89Zr-DFOSq, and for [124I]I-labeled APOMAB (124I), were 78.5%, 74.6% and 75.4%, respectively (b). The chDAB4 mAb radiolabeled with [89Zr] ZrIV or [124I] I is stable over 6 days in FCS at 37 °C as determined by ITLC (c). All data points are means ± SD

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