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Fig. 2 | EJNMMI Radiopharmacy and Chemistry

Fig. 2

From: Development of a fluorine-18 radiolabelled fluorescent chalcone: evaluated for detecting glycogen

Fig. 2

Fluorescence microscopy of compound 5 (green fluorescence), nuclear staining (blue fluorescence) in four cancer cell lines. Compound 5 was also incubated with α-amylase in IGROV-1 (high glycogen content) and HCT116 (low glycogen content) cells to digest carbohydrates. Images were obtained under × 400 magnification. Scale bar = 50 μm. Inset graph: Total intracellular glycogen levels determined by a modified glucose (GO) assay kit. Cells were seeded in 6-well plates at appropriate densities and allowed to attach overnight before determination of glycogen levels. Data are mean ± SD (n = 3)

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