Table 2 [¹³N]Ammonia TLC method validation results

[¹³N]Ammonia – TLC Strip #1

99

20.3

81.8

50.8

0.846

0–40

[¹³N]Ammonia – TLC Strip #2

98.96

17.7

79.2

51.7

0.862

0–40

[¹³N]Ammonia – TLC Strip #3

99.06

17.7

80.1

51.2

0.853

0–45

Mean

99.01%

0.854

[¹⁸F]Fluoride

%[¹⁸F]Fluoride integrated

Peak Start (mm)

Peak End (mm)

Peak centroid (mm)

Rf Value

Resazurin Standard Range (mm)*

[¹⁸F]Fluoride – TLC Strip #1

99.5

− 17.3

30.5

7.1

0.119

0–41

[¹⁸F]Fluoride – TLC Strip #2

99.78

−16.5

25.4

4.3

0.072

0–44

[¹⁸F]Fluoride – TLC Strip #3

99.77

−15.6

30.5

5.7

0.095

0–44

Mean

99.68

0.095

[¹³N]NOx

% [¹³N]NOx integrated

Peak Start (mm)

Peak End (mm)

Peak centroid (mm)

Rf Value

Resazurin Range (mm)*

[¹³N]NOx – TLC Strip #1

98.34

−12.4

14.4

0.1

0.00

0–40

[¹³N]NOx – TLC Strip #2

97.94

−13.2

13.5

−0.8

−0.01

0–41

[¹³N]NOx – TLC Strip #3

98.21

−14.1

14.4

− 0.6

−0.01

0–40

Mean

98.16

−0.01

[¹⁸F]Fluoride / [¹³N]Ammonia Mixed Solution (Co-spot)

% integrated

Peak Start (mm)

Peak End (mm)

Peak centroid (mm)

Rf Value

Resazurin Range (mm)*

[¹⁸F]Fluoride (4% impurity)

3.6%

−12.1

24.75

18

0.3

0–44

[¹³N]Ammonia (96% purity)

96.4%

31.5

80.1

49.5

0.82

Total

100%

Accuracy of [¹⁸F]Fluoride measurement

90% of expected value

Accuracy of [¹³N]Ammonia measurement

100.4% of expected value