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Table 3 The final HPLC conditions proposed

From: An alternative methodology for the determination of the radiochemical purity of 11C-methionine

Column Macherey-Nagel - EC250/4.6 NUCLEODUR 100–5 C18ec (MN)
Flow 1.0 mL/min.
Run time 16 min.
Solvent A KH2PO4 1,4 g/L
Solvent B CH3CN
Gradient % A % B
 0 to 6 min 99 to 98 1 to 2
 6 to 8 min 98 to 20 2 to 80
 8 to 16 min 20 80