Fig. 6
From: An updated radiosynthesis of [18F]AV1451 for tau PET imaging
Radio-TLC (right) and -HPLC (left) data from manual [18F]AV1451 radiolabeling reactions, showing formation of the [18F]-N-Boc-protected intermediate 4, followed by the [18F]AV1451 product 2. Aliquots were pulled at t = 0, 1, 5, and 10 minutes and analyzed using both methods [Radio-TLC analysis was performed using a Bioscan AR 2000 Radio-TLC scanner with EMD Millipore TLC silica gel 60 plates (3.0 cm wide x 6.5 cm long) developed using hexane:ethyl acetate (1:1); Radio-HPLC analysis was performed using a Shimadzu LC2010 HPLC equipped with radioactivity and UV detectors (column: Luna C18 5 micron 4.6x150 mm; MeCN:H2O gradient: 5:95 (0–3 min) – 5:95 → 95:5 (3–20 min) – 95:5 → 5:95 (20–30 min); flow rate: 1.5 mL/min; UV: 254 nm). Note: [18F]fluoride tR tended to fluctuate with the unbuffered H2O/MeCN HPLC gradient, and is thus present at different times in the HPLC rad traces (4–11 min), while small peaks observable in HPLC traces of t = 0 and 1 minutes are artifacts]