Skip to main content

Table 2 Representative studies evaluating 99mTc-labeled nanoparticles in preclinical cancer models

From: Radiolabeled nanomaterials for biomedical applications: radiopharmacy in the era of nanotechnology

99mTc-labeled NPs NPs/Chelate Experimental conditions t(min)/T(ºC)/pH Radiochemical yield (%) Evaluated applications References
[99mTc]Tc-IO-NPs-RGD IO-NPs/cRGDfK-Orn3-CGG 30 min/25 °C/pH 8  > 98% Molecular imaging of ανβ3-mediated tumor expression and feasibility for hyperthermia treatment. In vitro and in vivo results Tsiapa et al. 2014)
[99mTc]Tc-NPs-FA Oligomeric FA-NPs/chelate-free 30 min/25 °C Cell uptake by folate receptor-positive tumor targeting. In vitro results on HepG2 tumor cells Liang et al. 2018)
[99mTc]Tc-EDDA/HYNIC-GGC-AuNP-mannose AuNPs/EDDA-HYNIC 20 min/100 °C  > 95% Biodistribution and microSPECT/CT images of sentinel lymph node detection. In vivo results in Wistar rats Ocampo-García et al. 2011)
[99mTc]Tc-{(Au0)200-G5.NHAc-DOTA-FI-mPEG-(PEG-duramycin)} DENPs Au-DENPs/DOTA 30 min/25 °C 99% SPECT/CT imaging of chemotherapy-induced tumor apoptosis. In vitro and in vivo results Xing et al. 2018)
[99mTc]Tc-citrate-AuNPs AuNPS/chelate free 95.20 ± 2.70% Biodistribution patterns. In vivo results on solid tumor-bearing mice Essa et al. 2020)
[99mTc]Tc-PC:PEG2000-DSPE:SDC:DTPA-PE Micelles/DTPA-PE 30 min/25 °C 87 ± 1.21% Potential radiotracers for detection of infection/inflammation. In vitro results against S. aureus and E.coli Silindir-Gunay and Ozer 2020)
[99mTc]Tc-DSPE-PEG2000-DTPA PM Polimeric micelles/DTPA 15 min/25 °C/pH 7 93.8 ± 2.1% Biodistribution and scintigraphic images. In vitro and in vivo results on 4T1 tumor-bearing mice Oda et al. 2017)
[99mTc]Tc-HMPAO-blue-biotin-liposome Liposome/HMPAO 30 min/25 °C 92.1 ± 1.9% Scintigrams of the sentinel lymph node. In vivo results Phillips et al. 2001)
[99mTc]Tc-HMPAO-liposome Liposome/HMPAO 30 min 91% Tumor imaging with gamma camera. In vivo results Goins et al. 1994)
[99mTc]Tc-liposome Liposome/chelate-free 20 min/25 °C > 95% Biodistribution and scintigraphic images. In vivo results on CD1 mouse bearing breast cancer Navarro et al. 2011)
[99mTc]Tc-SpHL-DTPA-folate-PTX (folate-coated long-circulating and pH-sensitive liposomes)
[99mTc]Tc-SpHL-DTPA-PTX
Liposome/DTPA
Liposome/DTPA
15 min/25 °C/pH 7.4
15 min/25 °C/pH 7.4
98%
98.4 ± 1.1%
Biodistribution and scintigraphic images. In vivo results on BALB/c nude mice bearing breast cancer Monteiro et al. 2018)
[99mTc]Tc-oxine-SLNs
[99mTc]Tc-oxine-CH-SLNs
SLN/oxine
SLN/oxine
1 h/25 °C
1 h/25 °C
100%
100%
Biodistribution patterns. In vivo results on BALB/c mice Gharibkandi et al. 2019)
[99mTc]Tc-PEG-MnOx-MSNs MSNs/chelate-free 60 min/37 °C/pH 7.0 99.1 ± 0.6% SPECT-MRI dual-modal imaging (nanotheranostics). In vitro and in vivo results on tumour-bearing mice Gao et al. 2016)
[99mTc]Tc-DTPA-MSNs MSN/DTPA 5 min/25 °C/pH 7.0 98.3 ± 0.7% Biodistribution and scintigraphic images. In vivo results on healthy Swiss mice Barros et al. 2015)