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Table 5 Fraction (%) of mobile 99mTc species in radiolabelled glucose determined with TLC

From: An investigation of aspects of radiochemical purity of 99mTc-labelled human serum albumin nanocolloid

  [1] [2] [1] [2] [1] [2] [1] [2] [1] [2]
GBq mL−1 99mTc 0.75 0.04 0.75 0.04 0.75 0.04 0.75 0.04 0.75 0.04
TLC conditions TLC SG-60
Acetone
30–35 min
TLC SG-60
MEK
14–15 min
TLC-SA
85% methanol
14–15 min
TLC SG-60
85% methanol
30–32 min
TLC SG-60
0.9% NaCl
14–15 min
Identification Free [99mTc]TcO4 Free [99mTc]TcO4 Free [99mTc]TcO4 and 99mTc-glucose Free [99mTc]TcO4 and 99mTc-glucose Free [99mTc]TcO4 and 99mTc-glucose
t0 16.0 3.3 25.7 3.1 53.8 39.0 36.1 36.1 48.1 36.5
+ 30 min   1.6   2.2   19.3   17.2   19.1
+ 60 min   2.2   2.0   12.9   12.4   12.5
  1. Determined for two individual radiolabellings with different activity concentration of 99mTc (15 mg glucose, 40 µg Sn2+, 1.5–2 mL of 0.9% NaCl)