EJNMMI Radiopharmacy and Chemistry

Table 5 Fraction (%) of mobile ^99mTc species in radiolabelled glucose determined with TLC

From: An investigation of aspects of radiochemical purity of ^99mTc-labelled human serum albumin nanocolloid

	[1]	[2]	[1]	[2]	[1]	[2]	[1]	[2]	[1]	[2]
GBq mL^{−1 99m}Tc	0.75	0.04	0.75	0.04	0.75	0.04	0.75	0.04	0.75	0.04
TLC conditions	TLC SG-60 Acetone 30–35 min		TLC SG-60 MEK 14–15 min		TLC-SA 85% methanol 14–15 min		TLC SG-60 85% methanol 30–32 min		TLC SG-60 0.9% NaCl 14–15 min
Identification	Free [^99mTc]TcO₄⁻		Free [^99mTc]TcO₄⁻		Free [^99mTc]TcO₄⁻ and ^99mTc-glucose		Free [^99mTc]TcO₄⁻ and ^99mTc-glucose		Free [^99mTc]TcO₄⁻ and ^99mTc-glucose
t₀	16.0	3.3	25.7	3.1	53.8	39.0	36.1	36.1	48.1	36.5
+ 30 min		1.6		2.2		19.3		17.2		19.1
+ 60 min		2.2		2.0		12.9		12.4		12.5

Determined for two individual radiolabellings with different activity concentration of ^99mTc (15 mg glucose, 40 µg Sn²⁺, 1.5–2 mL of 0.9% NaCl)

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