Table 1 Reagent setup to enable automated radiosynthesis of [¹⁸F]mBPET-1 on iPHASE Flexlab

QMA

Waters QMA Light (activated with 10 mL 0.5 M K₂CO₃)

Sep-Pak D

Phenomenex Strata-X C18 (conditioned with EtOH/H₂O)

vial 1

3.5 mg K₂CO₃ in 200 μL H₂O + 20.0 mg K₂₂₂ in 400 μL MeCN

vial 2

4.0 μL 2-azidoethyl-4-toluenesulfonate in 750 μL MeCN

vial 3

1.0 mL MeCN

vial 7

100 μL DMF

vial 11

10 mL H₂O

vial 12

1.0 mL DMSO

vial 13

1.0 mL DMSO

vial 17

4 mL H₂O

vial 18

1.0 mL MeCN

vial 19

6.0 mg Cu (CH₃CN)₄ PF₆ + 4.0 mg TBTA in 300 μL DMF

HPLC-2 flask

40 mL H₂O

reactor 2

3.0 mg precursor 5 (neat)