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Auger electrons for cancer therapy – a review



Auger electrons (AEs) are very low energy electrons that are emitted by radionuclides that decay by electron capture (e.g. 111In, 67Ga, 99mTc, 195mPt, 125I and 123I). This energy is deposited over nanometre-micrometre distances, resulting in high linear energy transfer (LET) that is potent for causing lethal damage in cancer cells. Thus, AE-emitting radiotherapeutic agents have great potential for treatment of cancer. In this review, we describe the radiobiological properties of AEs, their radiation dosimetry, radiolabelling methods, and preclinical and clinical studies that have been performed to investigate AEs for cancer treatment.


AEs are most lethal to cancer cells when emitted near the cell nucleus and especially when incorporated into DNA (e.g. 125I-IUdR). AEs cause DNA damage both directly and indirectly via water radiolysis. AEs can also kill targeted cancer cells by damaging the cell membrane, and kill non-targeted cells through a cross-dose or bystander effect. The radiation dosimetry of AEs considers both organ doses and cellular doses. The Medical Internal Radiation Dose (MIRD) schema may be applied. Radiolabelling methods for complexing AE-emitters to biomolecules (antibodies and peptides) and nanoparticles include radioiodination (125I and 123I) or radiometal chelation (111In, 67Ga, 99mTc). Cancer cells exposed in vitro to AE-emitting radiotherapeutic agents exhibit decreased clonogenic survival correlated at least in part with unrepaired DNA double-strand breaks (DSBs) detected by immunofluorescence for γH2AX, and chromosomal aberrations. Preclinical studies of AE-emitting radiotherapeutic agents have shown strong tumour growth inhibition in vivo in tumour xenograft mouse models. Minimal normal tissue toxicity was found due to the restricted toxicity of AEs mostly on tumour cells targeted by the radiotherapeutic agents. Clinical studies of AEs for cancer treatment have been limited but some encouraging results were obtained in early studies using 111In-DTPA-octreotide and 125I-IUdR, in which tumour remissions were achieved in several patients at administered amounts that caused low normal tissue toxicity, as well as promising improvements in the survival of glioblastoma patients with 125I-mAb 425, with minimal normal tissue toxicity.


Proof-of-principle for AE radiotherapy of cancer has been shown preclinically, and clinically in a limited number of studies. The recent introduction of many biologically-targeted therapies for cancer creates new opportunities to design novel AE-emitting agents for cancer treatment. Pierre Auger did not conceive of the application of AEs for targeted cancer treatment, but this is a tremendously exciting future that we and many other scientists in this field envision.


Many radionuclides commonly used for imaging in nuclear medicine (e.g. 99mTc, 123I, 111In, 67Ga; Table 1; Fig. 1) decay by electron capture (EC) and/or internal conversion (IC). As a result of these decay processes, these high atomic number elements eject a series of low energy electrons in what is referred to as the Auger effect. Although Auger electrons originating from K-shell transitions can have energy higher than 25 keV, and up to 80 keV, their yield per decay is lower than 0.1. The majority of Auger electrons (AEs) have low energy (< 25 keV), which is deposited over short nanometre-micrometre distances in tissues. This extremely short range yields high linear energy transfer (LET), which makes AEs attractive for radiation treatment of cancer, especially if they are emitted in close proximity to cell sensitive targets such as DNA and the cell membrane. These high LET electrons were first described in independent work by Lise Meitner in 1922 (Meitner 1922) and Pierre Auger in 1923 (Auger 1923). Pierre Auger later published experiments to detect electron cascades from excited atoms in his published thesis (Auger 1926). Ionisation events were visualised in these early experiments by the use of cloud chambers, where ions produced in a dense water vapour were evidenced by the condensation of a water droplet upon liberation of an electron (Wilson 1923). Pierre Auger induced what is now known as the Auger effect in noble gases excited by incident X-rays, which resulted in a primary electron ejection event and multiple electron tracks (Auger 1975).

Table 1 Properties of Auger electron-emitting radionuclides a
Fig. 1
figure 1

Energy contribution of γ-photons, X-rays, β-particles, internal conversion (IC) electrons, and Auger electrons (AEs) per decay event for several radionuclides. Energy estimates are based on MIRD Radionuclide Data and Decay Schemes (Eckerman and Endo 2008), and the National Nuclear Data Center for 161Tb (65-Terbium-161 2011). * Number of K- and L- shell Auger electrons only

The Auger effect describes the process in which a vacancy in an inner electron orbital (i.e. K-shell) is filled by the decay of an electron from a higher shell (i.e. L-shell) with lower binding energy. The energy difference of this transition is emitted as a characteristic X-ray (Fig. 2a), or transferred to another electron which is subsequently ejected (Fig. 2b). When the ejected electron is from the same principle energy level (i.e. L-shell), it is referred to as an Auger electron, this results in two electron vacancies within the shell, for example resulting in 2s and 2p holes. When the ejected electron is from a higher shell (i.e. M-shell), resulting in a vacancy in two different principle energy levels (i.e. holes in 2s and 3s) it is referred to as a Coster-Kronig electron, and if the resulting outer shell electron hole (i.e. 3s) is filled by an electron of the same primary shell and also ejects an electron from that same shell (i.e. holes in 3p and 3d), this is referred to as a Super Coster-Kronig electron (Howell 1992; McGuire 1975). Auger, Coster-Kronig and Super Coster-Kronig electrons are collectively referred to as Auger electrons and result from the propagation of the Auger process as a cascade of electron vacancies and ejections. The primary vacancy can be induced by an incident X-ray (as shown by Auger), or occur during the decay of unstable nuclei (radionuclides) through electron capture (EC) or internal conversion (IC) processes. EC is the process in which an inner, K-shell electron is absorbed by a proton-rich nucleus, converting the proton and absorbed electron to a neutron, maintaining the atomic mass but resulting in an excited state and a primary orbital vacancy (Intemann and Pollock 1967). IC occurs when a nucleus in an unstable excited state releases excess energy that is transferred to an inner orbital electron, overcoming its binding energy and ejecting the electron (Fig. 2c) with high kinetic energy (Choppin et al. 2002).

Fig. 2
figure 2

Auger electron emission can be initiated by electron capture (EC) or internal conversion (IC). In EC, protons capture an inner (K) orbital electron resulting in a primary electron vacancy. This vacancy Is filled by decay of a lower energy electron of a higher orbital (i.e. L-shell). The difference in the electronic binding energy of the two orbitals can either (a) result in the emission of a characteristic X-ray of energy equal to the electronic transition energy (EL-EK) or (b) be transferred to an electron of lower binding energy (Eb), imparting it with kinetic energy upon ejection from the atom as an Auger electron. Progressive higher shell vacancies occur in the electron shells due to these electron transitions (open circles). c IC occurs in the de-excitation of unstable nuclei that impart sufficient energy to an electron to result in its ejection as an IC electron with high energy, also resulting in an inner orbital vacancy

In this review, we describe the radiobiological properties of AEs that make them attractive for cancer therapy, discuss their radiation dosimetry and describe methods for labelling biomolecules with AE-emitting radionuclides for targeted radiotherapy of cancer. We further review the preclinical studies of Auger electrons for cancer treatment and the limited but promising clinical studies that have been performed.

Radiobiological properties

Particles employed in systemic radiation therapy

Key considerations in the selection of a radionuclide for systemic radiation therapy, i.e. radiation therapy using targeted biomolecules, are the energy of the emitted particles (e.g. α-particles, β-particles or AE or IC electrons), and the range of these particles in tissues. AEs have a very short range of energy deposition, offering the most precise irradiation for cancer treatment. These low energy electrons may travel up to several micrometres from the site of decay, but the majority of the electrons have sufficient energy to travel only nanometre distances (Reilly and Kassis 2010). AEs emitted from the lower electron shells, particularly propagating from K-shells, have the largest energies and travel the greatest distances, but these are produced in lower abundance than electrons ejected after transitions from the outer electron shells (M- through O-shell) which are ejected with reduced energies and travel only a few nanometres (Howell 1992). Due to the short range of AEs emitted in cascade, most result in a high LET between 1 and 23 keV/μm, which is potentially very potent for producing clustered damage in macromolecular targets of a cancer cell, particularly DNA and the cell membrane. This is different from much more energetic (MeV) β particles, which deposit most of their energy along a longer (millimetre) track length, but at the track end, some of these particles yield high LET within a nanometre range causing repairable individual DNA lesions. Additionally, most AE-emitting radionuclides emit a small number of IC electrons, with higher energies and ranges up to several millimetres, providing a longer-range effect (Eckerman and Endo 2008; Howell 1992). In comparison, α-particles deposit extremely high energy (several MeV) over 5–10 cell diameters (50–100 μm), resulting in very high LET of 50–230 keV/μm. Historically, β-particles have attracted interest for treatment of cancer due to their average long range of energy deposition of 2–10 mm in tissue, which depends on the β-particle energy. This long range causes a cross-fire effect, which irradiates not only the targeted cells but additional neighbouring cells that are within the range of the β-particles. This aids in homogenisation of the radiation dose across millimetre sized solid tumours, but can also lead to greater haematological toxicity (Carr 2004; Salem et al. 2005; Stabin et al. 2001). For example, the cross-fire effect of the β-particles emitted by 177Lu or 90Y (maximum range of 1.7 or 11 mm in tissue, respectively) (Lai et al. 2017), radionuclides that have been complexed to monoclonal antibodies for radioimmunotherapy (RIT) of cancer, contributes to dose-limiting non-targeted hematopoietic toxicity (Vallabhajosula et al. 2005) through irradiation of bone marrow cells adjacent to skeletal regions of nonspecific uptake, or skeletal metastases, as well as circulating radioactivity perfusing the bone marrow.

Targets for cytotoxicity from AEs

Radiation induced cell death may proceed via several different mechanisms (Fig. 3). Direct DNA damage may be caused by traversal of the DNA duplex by high LET particles such as AEs or α-particles. Alternatively, indirect damage to DNA may be inflicted by reactive hydroxyl radicals (ROS) that are generated by interaction of AE, α-particles, β-particles, γ-photons or X-rays with water molecules and consequent radiolysis. The cascade of electrons and resultant local generation of free radicals may result in the formation of a concentrated region of macromolecular damage, which in the case of a DNA target can result in multiple and complex DNA breaks. Unrepaired damage can then result in cell death by several pathways, e.g. apoptosis or mitotic catastrophe (Haefliger et al. 2005; Kriehuber et al. 2004a; Urashima et al. 2006). DNA is considered the main target for causing radiation-induced cell death and indeed, the greater the unrepaired DNA damage the higher the incidence of lethality (Chan et al. 1976; Schneider and Whitmore 1963). Radiation-induced DNA double strand breaks (DSBs) are recognised by the protein kinase ataxia telangiectasia mutated (ATM), and through ATM/p53 pathways result in cell cycle arrest or apoptosis (Ismail et al. 2005). However, tumour cells of epithelial origin commonly have deficient pro-apoptotic pathways and are mainly killed through mitotic catastrophe as a consequence of attempting to enter mitosis with damaged DNA (Eriksson and Stigbrand 2010). Phenotypic features of cells entering pre-mature mitosis are aberrant chromosomes, multiple nuclei and micronuclei (Bhattathiri et al. 1998).

Fig. 3
figure 3

Modes of cell death caused by Auger electron (AE) emission. AEs may cause DNA double-strand breaks (DSBs) by a direct effect or through an indirect effect mediated by hydroxyl free radicals (ROS) due to interaction with water molecules. AEs may also cause cell membrane damage leading to cell death. There is a localised short-range “cross-dose” effect of AEs on cancer cells which are directly adjacent to targeted cells, and a longer range “bystander” effect on more distant cells

Since DNA has historically been considered the principal target for radiation treatment of cancer (Burdak-Rothkamm and Prise 2009), the emission of AEs in or near the cell nucleus, and especially in close proximity to the DNA greatly amplifies the lethality of these electrons, particularly taking into account the subcellular range of these electrons (Faraggi et al. 1994; Fasih et al. 2012; Hoang et al. 2012). Indeed when decaying in close proximity to DNA, AEs emitted by 125I exhibited superior cell killing properties than the longer-range β-particles emitted by 131I (Chan et al. 1976). Kassis and Adelstein showed that the AEs emitted by 125I were most lethal to Chinese hamster V79 lung fibroblasts when emitted by 125I-iododeoxyuridine (125I-IUdR), a nucleoside analogue that is incorporated into DNA, than with 125I-iododihydrorhodamine which is localised in the cytoplasm (Kassis et al. 1987). Relative biological effectiveness (RBE) describes the ratio of the effectiveness of one form of radiation compared to another, with X-rays or γ-radiation often used for comparison. An RBE = 1 indicates that the radiation has equivalent biological effects (e.g. cytotoxicity) as X-rays or γ-radiation. For deterministic effects, AE have exhibited RBE in the range of 2–5 (Freudenberg et al. 2014; Kriehuber et al. 2004b; Regulla et al. 2002; Yasui et al. 2001). The high lethality of AEs emitted near the nucleus is apparent in a comparison of RBE considering whole cell or nuclear targeting. Cellular dose calculations for the AE-radionuclides 99mTc and 123I in rat thyroid PC Cl3 cells, assuming a whole cell or nuclear target for dose calculations, resulted in an increased RBE from 0.75 to 2.18, for 99mTc and from 1.87 to 3.43 for 123I (Freudenberg et al. 2014). The enhanced efficacy of AE in proximity to DNA is illustrated in one report that showed that the RBE for 125I incorporated into DNA was almost 8, and that these low energy AEs were equivalently cytotoxic as the 5.3 MeV α-particles emitted by 210Po (Rao et al. 1989). However, if AEs were emitted in the cytoplasm, the RBE was ~ 1, and similar to low LET radiation such as X-rays (Rao et al. 1990). A modelling study of dose deposition in the DNA duplex revealed higher energy deposition by the AEs emitted by 125I than 5 MeV α-particles, and that even at a distance in DNA as short as 5 base pairs from the AE emission, the energy deposition was reduced by a factor of 10 (Charlton 1986). Some insightful studies employed triplex-forming oligonucleotides or minor groove-binding ligands that hybridised to DNA labelled with the AE-emitters 125I or 111In, to illustrate the distant-dependent effects of AEs on causing DNA double-strand breaks (Karamychev et al. 2000; Lobachevsky et al. 2008; Panyutin and Neumann 1997). Panyutin and Neumann (1997) demonstrated using 125I-labelled DNA triplex forming oligonucleotides that the probability of DNA strand breaks was strongly correlated with distance from the position of radionuclide decay, with 90% of DNA breaks found within 10 base pairs (Panyutin and Neumann 1997). Free radical scavenging studies that employed dimethylsulfoxide (DMSO) to eliminate indirect DNA damage, illustrated that beyond a critical distance, the DNA DSBs induced by AEs were mediated mainly by indirect effects caused by oxygen free radicals (Balagurumoorthy et al. 2012; Lobachevsky et al. 2008; Panyutin and Neumann 1997).

Detection of DNA damage

Interaction of AEs or their induced reactive oxygen species (ROS) with DNA can cause complex and multiple DSB (Fig. 3). The accumulation of unrepaired DNA DSB is negatively correlated with cell survival (Cai et al. 2008) and the misrepair of these breaks from damaging AE can result in chromosomal aberrations, similarly associated with reduced cell survival (Beckmann et al. 1993; Woo et al. 1989). Assays for DSBs induced in cancer cells by AEs are thus useful for studying their cytotoxicity. Common assays used for radiation-induced DNA DSBs include γH2AX, comet assay, and pulsed-field gel electrophoresis (PFGE). In response to DSBs caused by ionizing radiation, the histone H2AX is rapidly phosphorylated (γH2AX) and accumulates at these sites of DNA damage (Mah et al. 2011). The phosphorylation of H2AX can be detected within one minute after exposure to ionizing radiation and reaches maximal levels within 10–30 min (Rogakou et al. 1998). γH2AX can be imaged by confocal immunofluorescence microscopy of the nucleus of cells and appears as discrete foci, which are a measure of radiation induced DNA DSB (Cai et al. 2009). Immunofluorescent imaging of γH2AX foci has proven useful in the study of AE-mediated DNA damage in cancer cells (Cai et al. 2008; Mah et al. 2011; Piron et al. 2014; Sedelnikova et al. 2002; Yasui et al. 2007). A direct correlation was found between the number of radionuclide decays and the number of foci observed for human glioblastoma SF-268 and fibrosarcoma HT-1080 cells treated with 125I-IUdR, demonstrating a similar response in two different cancerous cell lines (Sedelnikova et al. 2002).

The single cell gel electrophoresis assay (SCGE) or comet assay, allows assessment of DNA damage at the single cell level. Cells are embedded in agarose and lysed with detergents at high concentrations of salt. Application of an electric field resolves fragmented DNA as a “tail” beyond the large, supercoiled, undamaged DNA “head” (Olive and Banath 1993). The comet assay has been useful to assess DNA damage caused by AEs (Haines et al. 2001; Hoyes et al. 2001; Olive and Banath 1993; Pedraza-López et al. 2000; Piron et al. 2014; Reske et al. 2007). For example, the comet assay was used to show increased DNA damage caused by 99mTc-hexamethylpropylenamineoxime (HMPAO) over 99mTc gentisic acid when internalised into the cytoplasm or remaining bound to the cell membrane of lymphocytes, respectively (Pedraza-López et al. 2000). It was also used to demonstrate extensive DNA damage caused by 125I-IUdR or 123I-4′-thio-2′-deoxyuridine (123I-ItdU) incorporated into DNA (Olive and Banath 1993; Reske et al. 2007).

Pulsed field gel electrophoresis (PFGE) allows for improved separation of large DNA fragments beyond the capabilities of traditional gel electrophoresis by employing an alternating electric field. PFGE is useful for analysis of larger samples of DNA from a high number of cells, contrasting it from single cell gel electrophoresis. PFGE has been used to examine DSB caused by AEs from 125I-IUdR in hamster V79-379A cells (Elmroth and Stenerlöw 2005) and Chinese Hamster Ovary (CHO) cells (Iliakis et al. 1991).

Cross-dose, bystander effects and cell membrane damage

The cross-fire effect, most commonly attributed to β-particles, describes the irradiation of cells distant from those harbouring the radionuclide due to the long (several millimetres) range of these particles. Despite the short nanometre-micrometre range of AEs, there is a local cross-dose effect of AE-emitting radionuclides that deposits dose in tumour cells that are immediately adjacent to cells in which the radionuclide decays (Cai et al. 2010) (Fig. 3). This is mediated by the several micrometre range of some higher energy AEs and IC electrons. In addition, tumour cells with lethal DNA damage caused by radiation may release mediators that cause the death of distant non-irradiated cells through the bystander effect (Mothersill et al. 2018) (Fig. 3). The bystander effect has been observed for AEs in vitro in media transfer experiments in which growth medium from donor cells exposed to 123I-metaiodobenzylguanidine (123I-MIBG) was transferred to non-irradiated recipient cells causing decreased clonogenic survival of these cells (Boyd et al. 2006; Paillas et al. 2016). Diminished clonogenic survival and increased numbers of γH2AX foci in HCT116 colon cancer cells were observed by media transfer experiments following exposure of donor cells to 125I-labelled anti-epidermal growth factor receptor (EGFR) monoclonal antibodies (mAb) (Paillas et al. 2016). Other studies have shown greater inhibition of tumour growth in mice inoculated with a mixture of non-irradiated cells and pre-irradiated cells compared to non-irradiated cells alone, demonstrating an AE-mediated bystander effect in vivo (Xue et al. 2002).

Due to the short range of most AEs, considerable attention has been focused on delivery of AE-emitting radionuclides to the nucleus or DNA (historically considered the primary cellular target of radiation damage) of tumour cells to maximise their cytotoxic effects. However, it has been shown that internalisation into cancer cells and delivery to the cell nucleus is not obligatory for cell killing, and that the lethal effects of AEs may be induced indirectly by free radical-mediated pathways (Goddu et al. 1996; Narra et al. 1995). Targeting the cell membrane has been proven to be an effective strategy for killing cancer cells with AEs (Paillas et al. 2016; Pouget et al. 2008; Santoro et al. 2009) (Fig. 3). In in vitro experiments, non-internalising 125I-anti-carcinoembryonic (CEA) mAbs bound to the surface of HCT116 colon cancer cells generated ROS that caused re-organisation of lipid rafts and activated receptor-mediated cell signalling pathways (ERK1/2, AKT, p38/JNK) and several phosphorylated protein mediators of Ca2+ levels (phospholipase C-γ and proline-rich tyrosine kinase 2 and paxillin) (Paillas et al. 2016). Cell membrane damage further induced γH2AX foci in the nucleus of donor cells exposed to 125I-anti-CEA mAbs and in recipient, non-exposed cells through a bystander effect. This study further revealed that DNA damage was quite homogeneous in CEA-positive A431 tumours in mice administered 125I-anti-CEA mAbs, despite radioactivity being localised mainly at the periphery of the tumour, suggesting a local bystander effect on non-targeted cells that could be mediated by damage to the cell membrane of targeted tumour cells (Paillas et al. 2016). 125I-labelled anti-CEA 35A7 was also found to be effective in vivo for treatment of small peritoneal tumours in mice, illustrating that internalisation and nuclear importation are not always required for the use of AEs for cancer therapy (Santoro et al. 2009). These findings are promising since they extend the targets for AE radiotherapy of cancer to non-internalising cell surface antigens overexpressed on tumour cells that are recognised by mAbs or other ligands.

Dosimetric properties

Organ and cellular dosimetry of AEs

Radionuclides that emit AEs also release γ-rays and X-rays and IC electrons. AEs have energies from a few eV to tens of keV, and ranges in soft tissue from a few nm up to 100 μm, while IC electrons have higher energies (tens to hundreds keV) and ranges (tens of μm to several mm). In contrast, γ-rays and X-rays are penetrating and travel much farther, mostly much longer than cm distances, except that for ultra-soft X-rays, the range can be as low as a few μm (Eckerman and Endo 2008; Berger et al. 2005; Hubbell and Seltzer 2004). Due to the diverse radiations and energy deposition distances and the dimension of critical targets, which range from single cells and subcellular compartments, to tumour masses and normal organs, in order to understand the whole picture of dosimetry for AE emitting radionuclides, both organ and cell doses should be considered (Bolch et al. 2009; Goddu et al. 1997; Loevinger et al. 1988; Roeske et al. 2008; Stabin 2006; Thierens et al. 2001; Vaziri et al. 2014). Organ dosimetry estimates the absorbed dose at the whole organ level, to which photons are the main contributor when the target organ is different than the source organ. Electrons play the prominent role when the source and target organs are the same, i.e. radioactivity localised in a source organ deposits energy in that same organ. However, when we zoom in one million times to examine the absorbed dose at the subcellular level, we need cellular dosimetry. This is especially the case for short range radiation such as AEs. Cellular dosimetry is the study of energy deposition at the cellular level, which considers both the cellular targets and sources (e.g. cell, cell membrane, cytoplasm and nucleus) as a quantitative means of understanding the biophysical interactions of radiation with matter (Goddu et al. 1997; Roeske et al. 2008; Vaziri et al. 2014).

The Medical Internal Radiation Dose (MIRD) schema is the gold standard to estimate the internal absorbed dose from radiopharmaceuticals. It can be described by eq. 1:

$$ \mathrm{D}\left({\mathrm{r}}_{\mathrm{T}},{\mathrm{T}}_{\mathrm{D}}\right)=\sum \limits_{r_S}{\widetilde{\mathrm{A}}}_{\left({\mathrm{r}}_{\mathrm{s}},{\mathrm{T}}_{\mathrm{D}}\right)}{\mathrm{S}}_{\left({\mathrm{r}}_{\mathrm{T}}\leftarrow {\mathrm{r}}_{\mathrm{S}}\right)} $$

where D (rT,TD) is the mean absorbed dose to the target region (rT) over a dose-integration period (TD), from a radionuclide distributed uniformly within a source region (rS). Ã (rS, TD) is the time-integrated radioactivity in rS over TD, while S (rT ← rS) is the absorbed dose in rT per radioactive decay in rS. The MIRD schema is applicable to both organ and cellular dosimetry (Dewaraja et al. 2012; Goddu et al. 1997; Siegel et al. 1999; Vaziri et al. 2014).

To calculate à (rS, TD), it is necessary to measure the source radioactivity at different time points to produce the time-radioactivity curve, followed by integration of this curve over TD. For clinical studies using AE emitting radionuclides such as 111In (Fisher et al. 2009; Vallis et al. 2014), 99mTc (Ocampo-Garcia et al. 2017) and 123I (Chin et al. 2014), planar imaging, SPECT/CT, non-imaging whole body radioactivity monitoring, or tissue sampling (e.g. blood and urine) may be used to assay the source radioactivity (Siegel et al. 1999). For animal studies, besides these imaging approaches used in the clinic, the source radioactivity is most commonly quantified by harvesting samples of source organs after euthanising animals at selected time points, then measuring the radioactivity in these tissue samples and the weight of the tissue sample to calculate the radioactivity per gram of tissue (Cai et al. 2016; Razumienko et al. 2016). Since organ dosimetry is based on à (rS, TD) in the entire organ, these tissue concentrations are multiplied by the organ weights to obtain the radioactivity per organ. For cellular dosimetry, cells are incubated with the radiopharmaceutical for different times, then cell fractionation is performed to separate the radioactivity bound to the cell membrane, internalised into the cytoplasm or imported into the nucleus, followed by measuring the radioactivity in each of these fractions by γ-counting (Ngo Ndjock Mbong et al. 2015). The selection of time points depends on both the biological (Tb) and physical (Tp) half-lives of the radiopharmaceuticals, which can be summarised by the effective half-life (Te):

$$ {\mathrm{T}}_{\mathrm{e}}={\mathrm{T}}_{\mathrm{b}}\ {\mathrm{T}}_{\mathrm{p}}/\left({\mathrm{T}}_{\mathrm{b}}+{\mathrm{T}}_{\mathrm{p}}\right) $$

At least 3 time points (e.g. ~ 1/3, 1 and 3 × Te) should be selected for each exponential term of the curve (Siegel et al. 1999). For example, 5 time points at 0, 4–6 h; 1, 3, 6 days post-injection (p.i.) of 111In-ibritumomab tiuxetan (Zevalin) mAbs were selected for quantitative SPECT to estimate the organ doses in a study of 10 lymphoma patients (Fisher et al. 2009). A shorter time period was used for estimating the doses from 111In-DTPA-human epidermal growth factor (111In-DTPA-hEGF) in 15 patients with EGFR positive breast cancer using whole body planar images acquired at 1, 4–6, 24 and 72 h p.i., since radiopeptides are eliminated more rapidly than mAbs (Vallis et al. 2014). Whole-body images were obtained at 20 min, 2, 6, 24 h post injection of 99mTc-EDDA/HYNIC-Tyr (3)-octreotide to estimate the radiation doses in 4 healthy individuals (Ocampo-Garcia et al. 2017). The choice of collimators and scan time, as well as attenuation and scatter correction used in image reconstruction all have effects on the accuracy of image quantification (Dewaraja et al. 2012; Siegel et al. 1999). Sub-organ regional dosimetry for AE-emitting radionuclides is desirable for critical organs such as the kidneys and liver, as well as the tumour due to the spatial non-uniformities of radioactivity distribution (Dewaraja et al. 2012). This is particularly true for the kidneys, since it has been found that the sub-organ distribution greatly influences the nephrotoxicity of radiopeptides, due to differences in the range of the β-particles emitted by 90Y or 177Lu and the AEs emitted by 111In and the distance between radioactivity localised in the renal tubules and the more radiosensitive glomeruli (Konijnenberg et al. 2004). For patients, it might be challenging to acquire SPECT/CT with sufficient resolution to accurately quantify radioactivity at different regions in critical normal organs or a tumour. The spatial resolution of clinical SPECT is 5–10 mm (Dewaraja et al. 2012). Recently resolutions of 2.5 and 1.6 mm were reported in images of a phantom using a multi-pinhole clinical (Chen et al. 2018) or preclinical (Massari et al. 2019) SPECT system, respectively. However, for animal studies, it is possible to perform autoradiography in combination with immunohistochemical staining of excised tissues ex vivo to understand the heterogeneous radioactivity distribution in different regions of tumours and normal organs (Lee et al. 2010). In one study, to understand the effect of heterogeneous radioactivity distribution in the tumour on the treatment response, tumour cell spheroids were cultured and incubated with 111In-DTPA-hEGF or 111In-DTPA-trastuzumab, then fixed, frozen, and cryo-sectioned (Falzone et al. 2018, 2019). One section was used for micro-autoradiography to visualise the radioactivity distribution and the adjacent section was stained for γH2AX to assess DNA damage. à (rS, TD) can be calculated from the area under the curve (AUC) via numerical methods such as the trapezoidal rule, or analytical methods by fitting the time-radioactivity curve to a sum of exponentials, followed by integration from time 0 to TD. If the radioactivity of rS cannot be directly measured, its time-radioactivity curve may be derived via compartmental modelling of the measured data of other regions with which rS is physiologically interacted (Siegel et al. 1999). Recently a robust biodistribution reporting and publication standard were proposed (Kesner and Bodei 2018) and an International Atomic Energy Agency (IAEA) Radiotracer Biodistribution Template (IAEA RaBiT) allowing detailed and standardised input of biodistribution data (Kesner et al. 2017) for dosimetry estimates is free to download (IAEA Radiotracer Biodistribution Template (RaBiT) n.d.). Figure 4 outlines the steps of acquiring à in vivo and in vitro for dosimetry estimates.

Fig. 4
figure 4

Outline of the steps to acquire time-integrated radioactivity (Ã) in vivo in tumours and normal organs, and in vitro in cancer cells for macro- or micro-dosimetry estimates, respectively

S-values depend on the physical properties of the radionuclides, and the size and geometry of source and target regions. They can be calculated either via Monte Carlo simulation or analytical methods in the case of cellular dosimetry. Figure 5 shows representative models of reference man (A), the kidney (B), or cells (C) used in Monte Carlo Simulation of S-values (Stabin and Siegel 2018; Bouchet et al. 2003; Cai et al. 2017). Monte Carlo code used to calculate S-values includes MCNP6 (Di Maria et al. 2018), PENELOPE (Falzone et al. 2017; López-Coello et al. 2019), Geant4 (López-Coello et al. 2019) and GATE (López-Coello et al. 2019). They all allow event-by-event simulation of electron and photon transport. Though Monte Carlo code is mostly used in condensed history mode for organ dosimetry, event-by-event simulation is more accurate for cellular dosimetry and is required for electrons with energies lower than 1 keV (Tajik-Mansoury et al. 2017). Monte Carlo simulation is more accurate but also more time consuming and computationally demanding. However, S-values for organs of several human phantoms, mice and rat tissues, cells and cell compartments are available in MIRD Pamphlets (Bolch et al. 1999; Bouchet et al. 1999, 2003; Goddu et al. 1997; Vaziri et al. 2014), ICRP reports (Mattsson et al. 2015) and numerous other publications. IDAC-Dose2.1 (Abuqbeitah et al. 2018; Andersson et al. 2017), an internal dosimetry computer program for diagnostic nuclear medicine based on the ICRP adult reference voxel phantoms (both male and female), is free software (IDAC-Dose2.1 n.d.) that allows calculation of the absorbed doses based on measured Ã. When human dosimetry is extrapolated from animal experiments, the radioactivity in normal organs of a human could be estimated from that in the respective organs of animals using the %kg/g method (Kirschner et al. 1975): (%ID/organ)human = [(%ID/organ)animal × (animal body weight/animal organ weight) × (human organ weight/human body weight). Another free software package, MIRDcell V2.1 (MIRDcell, a Multicellular Dosimetry Tool n.d.), a Multicellular Dosimetry tool, calculates cellular doses where the source is defined as the whole cell, cell surface, cytoplasm and nucleus and the target as the whole cell, nucleus or cytoplasm with cell and nucleus radius, as well as distance between cells adjustable in 1 μm increments. The cell and nucleus shape can only be defined as concentric spheres. The multicellular geometry can be either single cell, 1-D (dimensional) cell pair, 2-D colony or a 3-D cluster with sphere, rod, cone or ellipsoid shape. Cell labelling can be selected as uniform, log-normal or normal distribution (Vaziri et al. 2014). S values calculated with MIRDcell correlated well with those by Monte Carlo simulation, however, the relative error can be as high as 68%, depending on the source and target sizes as well as particle ranges and simulation codes. Comparable scoring size and particle range leads to the biggest differences between different Monte Carlo simulations (Tajik-Mansoury et al. 2017). Olinda/EXM (Stabin et al. 2005) is more widely used than IDAC-Dose2.1, but is not free software. Olinda/EXM 1.0 includes 10 phantom models; adult male, adult female, 1, 5, 10 and 15-year old, newborn, 3-, 6-, 9-month pregnant woman as well as models of the prostate gland, peritoneal cavity, spheres (used for tumours), head and kidneys. The anthropomorphic phantoms define the body and its organs with geometrical shape. Olinda/EXM 2.0 replaces the above phantoms with realistic, non-uniform rational B-splines (NURBS) voxel-based models and uses updated decay data. It also includes phantoms for the mouse, rat and dog. IDAC-Dose2.1, MIRDcell V2.1 and Olinda/EXM all use radiation spectra obtained via the (MIRD) RADTABS program. Nuclear Decay Data in the MIRD Format ( also produces continuingly evaluated radiation spectra of radionuclides including Auger electron emitters. However, it should be noted that the (MIRD) RADTABS program provides detailed Auger and Coster-Kronig electron spectra while the National Nuclear Data Centre only lists average energies and yields of Auger-K and Auger-L electrons. Recently it was reported that the yield of Auger, CK electrons from (MIRD) RADTABS was consistently higher than from the newly developed stochastic atomic relaxation model BrIccEmis (Falzone et al. 2017). The difference in spectra had a significant impact on the S-values only at the nanoscale.

Fig. 5
figure 5

Representative phantoms used in Monte Carlo Simulation for S-values of human organs, important kidney regions and subcellular compartments in a hexahedrally closely packed monolayer of cells: (a) a voxel-based realistic female phantom applied by OLINDA/EXM version 2.0 software (Stabin and Siegel 2018). b Kidney created using geometric shapes to represent renal pelvis, renal cortex and medullary pyramids (Bouchet 2003). c Cellular phantom with cell and cell nucleus represented by co-centric spheres with cell and nucleus radius of RC and RN, respectively (Cai et al. 2017)

Due to the subcellular and cellular range of AEs, for a single cell, SN ← N > > SN ← Cy > SN ← CS. For a monolayer of cells or a cell cluster, this trend remains the same, but the difference becomes smaller due to the contribution of cross-dose (Cai et al. 2010). Although nuclear DNA is the most sensitive target for radiation, recent studies show that radiation damage to mitochondria and cell membrane leads to apoptosis or cell death (Bavelaar et al. 2018; Kirkby and Ghasroddashti 2015; Pouget et al. 2018). A Monte Carlo mitochondrial dosimetry study on the β- and γ-emitter, 131I showed 2 order higher S-values for the mitochondrial source to mitochondrial target compartment compared to the nucleus to nucleus (Carrillo-Cázares and Torres-García 2012). It would be interesting to calculate S-values for the cell membrane source to cell membrane target and mitochondrial source to mitochondrial target for AE-emitting radionuclides, for correlation with the cytotoxic effects.

The dosimetry of AEs is important for understanding their effects on radiation sensitive targets in cells, and for predicting their effectiveness for cancer treatment as well as any potential toxicities to critical organs (e.g. red marrow, kidneys, liver and spleen). Preclinically, cellular dosimetry may be estimated based on subcellular distribution of radioactivity measured by cell fractionation studies, and organ dosimetry may be estimated in mouse tumour xenograft models by organ biodistribution studies which can be combined with ex vivo cell fractionation to measure subcellular distribution (Costantini et al. 2007). Clinically, however, there are challenges in estimating subcellular doses from AEs, since information is not available on the subcellular radioactivity distribution. Nonetheless, tumour and normal organ dosimetry is feasible. It should nevertheless be realised that there are limitations to tumour dosimetry in predicting the therapeutic response from targeted radiotherapeutic agents in patients, due to heterogeneous tumour uptake, inaccuracies in dose estimates based on the imaging data, and radiobiological factors including the sensitivity of tumours to exponentially decreasing low dose rate radiation emitted by AEs and other forms of radiation used (in contrast to external radiation treatment which is much higher dose rate radiation). Organ dosimetry has nonetheless been found to be quite accurate in predicting normal tissue toxicity to the bone marrow and the kidneys in patients receiving targeted radiotherapeutic agents (Chalkia et al. 2015; Sarnelli et al. 2017; Strigari et al. 2014). Absorbed doses are only the first step to understand the dose-effect relationships. The microenvironments and radiosensitivity of the tumour and normal tissues, radiation chemistry reactions and radiobiological effects such as the bystander effect induced by physical dose absorption all contribute to the outcomes (Pouget et al. 2018; Ma et al. 2019; Dong et al. 2019).

Radiolabelling approaches

To achieve selective delivery of AE-emitting radionuclides to tumours for cancer treatment, these radionuclides must be attached to targeting ligands such as monoclonal antibodies (mAbs), or peptides that recognise cell surface receptors displayed on cancer cells (Fig. 6). In some cases, nanoparticles that incorporate polymers that complex AE-emitters and are modified on their surface with targeting ligands have also been used (Cai et al. 2016; Fonge et al. 2009; Hoang et al. 2012, 2013). Radiolabelling of mAbs and peptides with AE-emitters may be performed by radioiodination (125I or 123I), or by modification with bifunctional metal chelators (e.g. DTPA) that complex radiometals (111In, 67Ga, 64Cu; Table 1). Radioiodination requires oxidation of 125I or 123I from a valence state of − 1 as sodium iodide to + 1 for electrophilic substitution onto tyrosine amino acids in mAbs or peptides (Reilly 2007). Iodogen® (1,3,4,6-tetrachloro-3α,6α-diphenyl-glycoluril; Fraker and Speck Jr 1978) is the most commonly used oxidising reagent. However, since radioiodination efficiency is typically low (40–60%), radioiodinated mAbs and peptides must be purified post-labelling to remove free 125I or 123I. This does not allow kit formulation for preparing the radiopharmaceutical. Another disadvantage is that radioiodinated proteins are deiodinated in vivo by deiodinases, which are ubiquitous in the body and function to deiodinate thyroid hormones (Reilly 2007). In contrast, mAbs and peptides labelled with radiometals via chelation are relatively stable in vivo, and complexation of radiometals by chelators is very efficient (> 90–95%) enabling kit formulation. We previously reported the design of a kit for labelling DTPA-hEGF with 111In for AE radiotherapy of EGFR-positive breast cancer (Reilly et al. 2004). The most commonly used chelators for complexing AE-emitting radiometals are DTPA (diethylenetriaminepentaacetic acid), DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) and NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid). DOTA and NOTA are most suitable for labelling mAbs and peptides with 67Ga or 64Cu, but DOTA may also be used for 111In-labelling (Reilly 2007). DOTA and NOTA are macrocyclic chelators that provide a more stable radiometal complex than DTPA, but this causes slower labelling kinetics which requires mild heating of the DOTA-conjugated mAbs to 42 °C and longer incubation times (Razumienko et al. 2016). Peptides are more heat-stable than mAbs and thus DOTA-peptide conjugates may be heated up to 95 °C to achieve rapid and efficient complexation of radiometals (Brom et al. 2012). Several bifunctional chelators have been synthesised with a chemically-reactive group positioned on a side-chain for conjugation to mAbs to preserve the most stable octadentate radiometal-chelator complex. These include p-isothiocyanatobenzyl DTPA (p-SCN-Bn-DTPA), p-SCN-Bn-DOTA, and p-SCN-Bn-NOTA (Macrocyclics, Inc., Plano, TX, USA). The p-isothiocyanate group forms a thiourea linkage with ε-amino groups on lysines on the mAbs. Peptides may be assembled by solid-phase peptide synthesis which allows a chelator to be positioned at a specific location in the sequence to complex radiometals (Lever et al. 2019).

Fig. 6
figure 6

Examples of targeting vehicles for delivery of Auger electron (AE)-emitting radionuclides to cancer cells. Non-internalising monoclonal antibodies (mAbs) or peptides bind to cell-surface receptors and may kill cells through damaging the cell membrane. Internalising mAbs or peptides may be transported to the cell nucleus by appending nuclear localisation sequence (NLS) peptides or by an endogenous NLS present in some cell-surface receptors (e.g. EGFR or HER2) or combine internalising, endosomal escape and nuclear localizing sequences (modular nanotransporters). Nuclear localisation causes lethal DNA double-strand breaks (DSBs). Micelles or gold nanoparticles may be modified with mAbs or peptides to target cell surface receptors or are internalised non-specifically into cancer cells by endocytosis. 125I-labelled anthracyclines diffuse into cancer cells and intercalate into DNA, while 125I-2-iododeoxyuridine (125I-IUdR) is taken up by nucleoside transporters and incorporated into DNA. These agents cause lethal DNA DSBs

An important consideration for cancer treatment with AEs is the specific activity (SA) of the radiopharmaceuticals, since this defines the amount of radioactivity delivered to a single cancer cell per receptor-recognition event. At the SA usually achieved for labelling mAbs with 111In (< 1 MBq/μg), there may be as few as 1 in 50 antibody molecules that are radiolabelled, which limits their effectiveness for killing cancer cells, especially cells that have low-moderate target receptor expression (Ngo Ndjock Mbong et al. 2015). A strategy which we have explored to increase the SA of trastuzumab labelled with 111In is conjugation of the antibodies to G4 polyamidoamine (PAMAM) dendrimers derivatised with multiple DTPA (Chan et al. 2013). This approach increased the SA by 100-fold compared to conjugation directly with DTPA and increased the cytotoxicity in vitro of these radioimmunoconjugates (RICs) on HER2-positive breast cancer cells by up to 9-fold. Another approach is conjugation of the mAbs with metal-chelating polymers (MCPs) that present multiple DOTA chelators for 111In (Aghevlian et al. 2018; Ngo Ndjock Mbong et al. 2015). Conjugation of trastuzumab to MCPs presenting 24–29 DTPA chelators increased the SA of trastuzumab labelled with 111In by 90-fold compared to trastuzumab conjugated to DTPA and increased the cytotoxicity of these RICs in vitro on HER2-positive breast cancer (BC) cells, including cells with low-moderate HER2 expression (Ngo Ndjock Mbong et al. 2015). Panitumumab modified with an MCP that presented 13 DOTA chelators increased the SA for labelling with 111In to > 70 MBq/μg (Aghevlian et al. 2018).

Nanoparticles may be labelled with AE emitters by incorporation of polymers that present DTPA or DOTA for complexing 111In. For example, 111In-labelled block copolymer micelles (BCMs) modified with EGF to target EGFR-positive breast cancer cells were assembled by incorporating a DTPA-polyethylene glycol (PEG)-b-polycaprolactone (PCL) block polymer into these micelles (Fonge et al. 2009). 111In-labelled BCMs modified with trastuzumab to target HER2-positive breast cancer cells were similarly constructed (Hoang et al. 2012). We labelled gold nanoparticles (AuNPs) modified with trastuzumab by attaching 111In-DTPA-PEG polymers to the surface of the AuNPs via a gold-thiol linkage (Cai et al. 2016).

Preclinical studies

DNA-binding Radiotherapeutic agents

Since AEs are most damaging to DNA and lethal when emitted in close proximity to DNA, the pioneering work on AEs for cancer treatment was performed by Kassis and Adelstein using 125I- or 123I-5-iodo-2-deoxyuridine (IUdR) (Kassis et al. 1987; Makrigiorgos et al. 1989). 125I/123I-IUdR is a nucleoside analogue that is incorporated directly into DNA. Emission of AEs causes DNA DSBs resulting in chromosomal aberrations in DNA, causing profound cytotoxicity (Chan et al. 1976; Kassis et al., 1987). Chan et al. compared the clonogenic survival of V79 Chinese hamster (CHO) cells exposed in vitro to 125I-IUdR with 3H-deoxyuridine and 131I-iododeoxyuridine (3H-TdR; 131I-IUdR). 125I-IUdR yielded a steeply declining clonal survival curve with a small shoulder, typical of high-LET radiation, and 125I-IUdR was more cytotoxic than 3H-TdR or 131I-IUdR, which emit low energy or intermediate energy β-particles, respectively. Incubation of the cells for one hour with only 0.0037 Bq of 125I-IUdR generated 3 DNA DSBs per cell, but a 10-fold greater amount of 131I-IUdR and 17-fold higher amount of 3H-TdR were required for the same DNA damaging effect. The radiotoxicity of DNA-incorporating 125I-IUdR has also been shown in preclinical in vivo studies. Sahu et al. demonstrated that 125I-IUdR treatment prevented paralysis caused by leptomeningeal gliosarcoma metastases in rats (Sahu et al. 1997). A single intrathecal administration of 18.5 MBq of 125IUdR, given over 5 daily fractions or by continuous 5-day infusion prolonged time-to-paralysis to 11, 12 and 15 days respectively, from 9 days for control saline-treated mice. These early demonstrations of DNA-targeted AE radiotherapy in mammalian cell lines and aggressive preclinical animal tumour models illustrate the high cytotoxicity of AEs when emitted close to DNA.

More recently, a novel chemoradiotherapy strategy has been explored using AE-emitters. The chemotherapy drug cisplatin [cis-diaminedichloroplatinum (II)] forms DNA-platinum adducts in cells. This DNA crosslinking agent has been combined with radiotherapy, termed chemoradiotherapy, in the treatment of several solid cancers (Shrivastava et al. 2018; Psyrri et al. 2004; Zatloukal et al. 2004). Cisplatin and ionising radiation show synergism in their cell killing effects (Gorodetsky et al. 1998). Thus, combining cisplatin with radiotherapy using radionuclides of platinum that emit AEs (e.g. 195mPt and 191Pt) is a rational approach. 195mPt decay results in a larger number of AEs and a high energy per decay compared to other commonly used AE emitters such as 125I and 111In (Table 1). 195mPt produced from irradiation of 197Au in a high current linear accelerator [197Au(γ,np)195mPt] was extracted and used to synthesise 195mPt-cisplatin with a high specific activity of up to 3.7 TBq/mg by Bodnar and colleagues (Dykiy et al. 2007; Bodnar et al. 2015). Treatment of Ehrlich adenocarcinoma cells in vitro with 0.017 pg/mL 195mPt-cisplatin reduced cell viability to 3% within 6 h, an approximate 8-fold reduction compared to 7.5 μg/mL treatment with nonradioactive cisplatin, which decreased cell viability to only 25% (Bodnar et al. 2015). In vivo, male mice with subcutaneous Ehrlich adenocarcinoma xenografts in the right thigh received 5 intraperitoneal (i.p.) injections of normal saline (control) or 0.7 mg nonradioactive cisplatin on alternating days, or received a single injection of 0.017 pg 195mPt (Bodnar et al. 2015). Mice were monitored for body weight and tumour size for 21 days. The single treatment with 195mPt-cisplatin resulted in a 65% tumour growth inhibition, compared to 35% tumour growth inhibition with 5 treatments of nonradioactive cisplatin. While both nonradioactive and 195mPt-cisplatin treatments resulted in significant decreases in body weight compared to the normal saline treated control mice, there were no significant differences in the body weights between radioactive and nonradioactive cisplatin treatments. These promising results suggest that combining an AE emitter with cisplatin chemotherapy greatly enhances the therapeutic effect of cisplatin on tumours while not increasing normal tissue toxicity.

Another platinum isotope, 191Pt has been investigated for chemoradiotherapy. 191Pt decays with a half-life of 2.8 days by EC, producing 17.8 KeV of AEs per decay (and 273 KeV/decay in γ-photons) (Table 1, Eckerman and Endo 2008). Areberg et al. produced 191Pt by proton irradiation (75–65 MeV) of gold foil [197Au(p,2p,5n)191Pt, 197Au(p,p6n)191Au➔191Pt, 197Au(p,7n)191Hg➔191Au➔191Pt] and synthesised 191Pt-cisplatin (Areberg et al. 1999). The in vitro cytotoxicity of 191Pt-cisplatin was assessed at increasing specific activities of 0–167 MBq/mg (0–20 μg/mL) on ME180 human cervical carcinoma cells to determine the IC50 values (Areberg et al. 2000) which is the concentration required to inhibit cell growth by 50%. The IC50 was reduced from 3.2 μg/mL for nonradioactive cisplatin, to 2.8 and 0.8 μg/mL for the lowest (48 MBq/mg) and highest (167 MBq/mg) specific activities, respectively. This resulted in enhancement ratios of 1.2 to 4.3 for 191Pt-cisplatin compared to nonradioactive cisplatin. The enhanced potency of 191Pt-cisplatin was subsequently shown in tumour-bearing BALB/c nude mice (Areberg et al. 2001). A cisplatin-sensitive patient-derived squamous cell carcinoma of the nasal cavity was subcutaneously inoculated into male and female mice. Treatment groups consisted of mice receiving i.p. normal saline, 5 mg/kg nonradioactive cisplatin administered i.p., or a single i.p. injection of 80 MBq/mg or 160 MBq/mg of 191Pt-cisplatin. Specific tumour growth delay (SGD, the normalised difference in tumour doubling time compared to normal saline treated control mice) was 2.1 for nonradioactive cisplatin, 3.0 for 80 MBq/mg 191Pt-cisplatin, and 3.9 for 160 MBq/mg 191Pt-cisplatin. It was calculated that for the same tumour growth delay effect obtained with a 5 mg/kg dose of 80 or 160 MBq/mg 191Pt-cisplatin, the dose of nonradioactive cisplatin would need to be 9 and 10 mg/kg, respectively.

Other DNA-binding radiotherapeutic agents that emit AEs have been studied. Anthracyclines (e.g. doxorubicin and daunorubicin) are planar aromatic molecules that intercalate between base-pairs in DNA. Radiolabelled anthracyclines could cause DNA damage by AE emission in addition to the chemotoxicity of these drugs. Ickenstein et al. demonstrated in vitro the potent radiotoxicity of radioiodinated daunorubicin (Ickenstein et al. 2006). Exposure of SK-BR-3 human breast cancer cells to 125I-labelled daunorubicin (50 kBq/mL; 0.5 ng/mL) was more than 4-orders of magnitude more cytotoxic than unlabelled daunorubicin or doxorubicin, or stable 127I-iodinated daunorubicin, demonstrating the increased potency imparted on these anthracycline drugs by labelling with 125I. Imstepf et al. studied a doxorubicin derivative conjugated to a dipicolylamine chelator for radiolabelling with 99mTc tricarbonyl complex (Imstepf et al. 2015). The 99mTc-labelled doxorubicin produced a strong cytotoxic effect on radiosensitive murine melanoma B16F1 cells. After 36 h of incubation at the maximum concentration of 99mTc studied (10 MBq/mL), about 80% of B16F1 cells were killed, but only 30% and 25% of A431 human squamous carcinoma cells and HeLa cells were killed, respectively. The reduced cytotoxicity of 99mTc-doxorubicin in these cells may reflect differences in radiosensitivity to AEs. Nonetheless, incubation with 99mTcO4 which was not accumulated in the nucleus and did not bind to DNA, only killed about 10% of the cells for all three cell types. 99mTc is not ideal as an AE-emitter for cancer radiotherapy because it emits 4.4 AEs per decay but there is a low total AE energy released per decay (0.9 keV) and a low average energy per AE (0.2 keV) (Table 1). However, there is a higher energy IC electron with average energy of 13.8 keV and total energy released per decay of 15.2 keV.

Acridine and acridone derivatives are an additional class of DNA intercalators that have been studied as platforms for delivery of AE-emitting radionuclides. Desbois et al. screened a panel of acridine and acridone derivatives for cytotoxicity in several human and murine melanoma cell lines and identified 7-iodo-acridone, and 5-iodo-acridine as two compounds that demonstrated cytotoxicity in vitro on M4Beu human melanoma cells (Desbois et al. 2008). The biodistribution of these compounds was studied in mice bearing B16F0 murine melanomas. Both 125I-labelled compounds accumulated in B16F0 tumours at 72 h post-injection, but 7-125I-iodo-acridone exhibited 2-fold greater uptake (17.5 vs. 6.9%ID/g). Gardette et al. investigated 125I-labelled acridine (125I-ICF01040) and acridone (125I-ICF01035) derivatives in both melanotic and amelanotic melanoma cells (Gardette et al. 2014). 125I-ICF01035 was concentrated mainly in melanosomes of B16F0 murine melanoma cells, but in A375 amelanotic human melanoma cells, 125I-ICF01035 was taken up into the nucleus. Despite these differences in subcellular distribution, 125I-ICF01035 was similarly cytotoxic to both cell types (50% growth inhibitory activity, A50 = 10–12 kBq/mL). 125I-ICF01040 which mainly accumulated in the cytoplasm, had significantly greater cytotoxicity in all cell lines and was the most cytotoxic in B16F0 cells (A50 = 2 kBq/mL). This study demonstrated that although nuclear localisation resulted in the greatest cytotoxicity, cytoplasmic distribution also resulted in killing of melanoma cells in vitro.

Targeted Radiotherapeutic agents

The epidermal growth factor receptor (EGFR) is the first member the EGFR family which also includes HER2, HER3 and HER4 (Olayioye et al. 2000). EGFR overexpression is a hallmark of many epithelial-derived cancers (Salomon et al. 1995). Michel et al. reported killing of EGFR-positive vulvar squamous cell carcinoma A431 cells in vitro by 111In- or 125I-labelled anti-EGFR antibodies (Michel et al. 2004). At the highest concentration studied (1.5 MBq/mL), 93–100% of these cells were killed. 131I-anti-EGFR antibodies also killed A431 cells, but these effects were not targeted, and mostly resulted from β-particle emissions in the growth medium (i.e. cross-fire effect). As discussed earlier, nuclear importation is an important property that maximises the lethal DNA damaging effects of AEs. Our group modified 111In-labelled anti-EGFR mAb nimotuzumab with 13-mer peptides CGYGPKKKRKVGG that harbour the SV-40 large T-antigen-derived nuclear localisation sequence (NLS; italicised) and studied the cytotoxicity of these radioimmunoconjugates towards EGFR-positive breast cancer cells (Fasih et al. 2012). The NLS peptides are recognised by importins α/β that function to shuttle cytoplasmic proteins across the nuclear pore complex (Costantini et al. 2008b). NLS peptide modification enhanced nuclear uptake 2-fold in EGFR-positive MDA-MB-468 human breast cancer cells compared to 111In-nimotuzumab without NLS. However, 111In-nimotuzumab without NLS still accumulated in the nucleus, probably mediated by an endogenous NLS in the transmembrane domain of the EGFR (Wang and Hung 2012). Nonetheless, the incorporation of NLS rendered 111In-NLS-nimotuzumab 7-fold more potent in reducing the CS of MDA-MB-468 cells and caused 2-fold more DNA DSBs detected by immunofluorescence for γH2AX foci in the nucleus (Fasih et al. 2012).

111In-DTPA-hEGF is an AE-emitting radiotherapeutic agent for EGFR-positive breast cancer previously studied by our group (Reilly et al. 2000).111In-DTPA-hEGF exhibited rapid receptor-mediated binding and internalisation into MDA-MB-468 cells and importation into the cell nucleus within 24 h, likely mediated by the endogenous NLS in the EGFR. MDA-MB-468 cells exposed to 111In-DTPA-hEGF exhibited decreased CS which was directly correlated with increased density of DNA DSBs in the cell nucleus assessed by immunofluorescence for γH2AX (Cai et al. 2008). 111In-DTPA-hEGF administered i.v. in fractionated doses over 5 weeks (total 28–92 MBq; 5–17 μg) significantly inhibited the growth of MDA-MB-468 tumours in mice, and was particularly effective for treatment of small tumour xenografts (Chen et al. 2003). Some decreases in white blood cell (WBC) and platelet counts were observed, but these remained within the normal range. There was no hepatic or renal toxicity. Subsequent preclinical translational bridge studies revealed that administered amounts of 111In-DTPA-hEGF up to 44 MBq (3–30 μg) in mice or 85.1 MBq (58 μg) in rabbits, corresponding to 44-times and 1-times the maximum planned human administered amount of radioactivity for a Phase 1 clinical trial were safe (Reilly et al. 2004). The projected whole-body effective dose in humans was 0.19 mSv/MBq and the projected doses to the liver and kidneys were within safe limits for the Phase 1 trial (see Clinical Studies section). Despite these encouraging results, bioactive peptides such as hEGF may cause adverse effects in humans when used for radiotherapy. In the subsequent Phase 1 trial of 111In-DTPA-hEGF, patients experienced flushing, hypotension and nausea, which was attributed to the hEGF moiety (see Clinical Studies section) (Vallis et al. 2014). We studied an 111In-labelled truncated form of hEGF (111In-EGFt) that exhibits decreased biological activity (Panosa et al. 2015). 111In-DTPA-EGFt bound with lower affinity to the EGFR than 111In-DTPA-hEGF (Kd = 6.0 × 10− 8 M vs. 1.3 × 10− 9 M, respectively) but nevertheless was internalised and imported into the nucleus of MDA-MB-468 cells. However, 111In-DTPA-EGFt was 2–8 fold less cytotoxic to MDA-MB-468 cells in vitro than 111In-DTPA-hEGF and exhibited 2.2-fold lower tumour uptake in vivo in mice with MDA-MB-468 xenografts.

HER2 is the second member of the EGFR family. HER2 is overexpressed on 15–25% of breast cancers and is the target for treatment with trastuzumab (Herceptin; Roche), pertuzumab (Perjeta; Roche) and trastuzumab-emtansine (Kadcycla; Roche) (Larionov 2018). We modified trastuzumab with NLS peptides and derivatised these immunoconjugates with DTPA to complex 111In for AE RIT of HER2-positive breast cancer (Costantini et al. 2007) (Fig. 7). 111In-DTPA-NLS-trastuzumab was bound and internalised by HER2-positive breast cancer cells and transported to the nucleus. Exposure of SK-BR-3 human BC cells in vitro to 111In-DTPA-NLS-trastuzumab (70 μg/mL; 240 MBq/mg) caused extensive DNA DSBs, reducing their clonogenic survival by > 90% (Costantini et al. 2007). In contrast, unlabelled trastuzumab (70 μg/mL) decreased the survival of SK-BR-3 cells by only 35%. 111In-DTPA-NLS-trastuzumab exhibited high tumour uptake (12.1%ID/g) at 72 h p.i. in mice with HER2-overexpressing MDA-MB-361 human breast cancer xenografts (Costantini et al. 2007). For RIT studies, an administered radioactivity amount level of 111In-DTPA-NLS-trastuzumab that caused no observable adverse effects (NOAEL) on hematopoietic, liver or kidney function and on body weight was first determined (Costantini et al. 2010). A single injection at the NOAEL (9.25 MBq; 4 mg/kg) administered to mice with subcutaneous MDA-MB-361 xenografts significantly inhibited tumour growth by 4-fold (Costantini et al. 2010) (Fig. 8a). An equivalent single mass dose (4 mg/kg) of unlabelled trastuzumab was 3-fold less effective than 111In-DTPA-NLS-trastuzumab. Treatment of mice with MDA-MB-361 tumours with two injections of 111In-DTPA-NLS-trastuzumab (9.25 MBq; 4 mg/kg each) separated by two weeks significantly prolonged survival compared to mice treated with unlabelled trastuzumab or untreated mice (Fig. 8b).

Fig. 7
figure 7

111In-NLS-trastuzumab is an example of a targeted Auger electron (AE)-emitting radioimmunotherapeutic (RIT) agent composed of the anti-HER2 monoclonal antibody, trastuzumab modified with nuclear translocation sequence (NLS in red) peptides and benzylisothiocyanate DTPA (BzDTPA) to complex 111In. 111In-NLS-trastuzumab is internalised by HER2-overexpressing breast cancer cells and is transported to the cell nucleus, where the AEs cause lethal DNA double-strand breaks (DSBs) (Costantini et al. 2007)

Fig. 8
figure 8

a Treatment of athymic mice with subcutaneous HER2-positive MDA-MB-361 human breast cancer xenografts with a single injection of 111In-NLS-trastuzumab (9.25 MBq; 4 mg/kg) significantly slowed tumour growth compared to control mice receiving unlabelled trastuzumab (4 mg/kg) or normal saline. b Treatment of tumour-bearing mice with two injections of 111In-NLS-trastuzumab (9.25 MBq; 4 mg/kg) significantly prolonged survival compared to control mice receiving unlabelled trastuzumab or normal saline (Costantini et al. 2010)

Our group extended this approach to RIT of acute myeloid leukemia (AML) using 111In-labelled anti-CD33 murine M195 and humanised HuM195 mAbs (Chen et al. 2006) similarly modified with NLS peptides to promote nuclear importation. 111In-NLS-HuM195 exhibited 2-fold greater potency than 111In-HuM195 without NLS for killing HL-60 human myeloid leukemia cells in vitro, and also decreased the clonogenic survival of CD33-positive primary patient AML specimens (Chen et al. 2006). Moreover, 111In-NLS-HuM195 retained its cytotoxicity in vitro against HL-60-MX-1 cells, which are a mitoxantrone-resistant subclone of HL-60 cells as well as on primary AML specimens that expressed multidrug resistance (MDR) transporters (Kersemans et al. 2008). These results suggest that RIT with AEs could be a promising approach to treatment of AML and may be able to overcome chemotherapy resistance, which is a challenge in treating this disease. Our subsequent studies focused on anti-CD123 (interleukin-3 receptor, IL-3, α-subchain) murine 7G3 or humanised CSL360 mAbs (CSL Ltd., Parkville, Australia) modified with NLS and labelled with 111In (Leyton et al. 2011). Expression of CD123 in the absence of CD131 (IL-3R β-subchain) is a phenotype that is expressed by leukemic stem cells (LSCs). LSCs are believed to cause AML and their survival after treatment is implicated in recurrence (Jordan et al. 2000). 111In-NLS-7G3 and 111In-NLS-CSL360 killed AML-5 human myeloid leukemia cells expressing the CD123+/CD131 phenotype of LSCs (Gao et al. 2016; Leyton et al. 2011). Combining 111In-NLS-CSL360 with inhibitors of DNA repair increased their cytotoxicity in vitro (Zereshkian et al. 2014). These RICs localised in the bone marrow (BM) and spleen and at other extramedullary sites of leukemia in NOD/SCID mice engrafted with AML-5 cells or primary AML specimens, detected by microSPECT/CT (Leyton et al. 2011, 2014). However, we encountered two challenges in studying RIT of AML with 111In-NLS-CSL360 preclinically in AML-engrafted mice. The mouse model which was used to identify LSCs and is also used to study new treatments for leukemia is the NOD/SCID mouse engrafted into the BM with primary AML cells (Bonnet et al. 1999). However, NOD/SCID mice harbour a germ-line mutation in DNA repair, which renders these mice unusually sensitive to radiation, including to RIT, and limits the amount of radioactivity which may be safely administered. Moreover, NOD/SCID mice require pre-treatment with 200 cGy of X-radiation to enable AML engraftment. We found that paradoxically, RIT with 111In-NLS-CSL360 encouraged AML engraftment rather than decreased engraftment, due to a boost in radiation to the BM caused by the γ-photons from 111In which further primed the BM niche for leukemic cell engraftment (Bergstrom et al. 2016). NRG (NOD-Rag1null IL2rgnull, NOD rag gamma) mice that do not harbour this DNA repair defect may be used to study RIT, but these mice are more immunocompromised than NOD/SCID mice and AML engraftment efficiency is often very high, resulting in a high tumour burden that is difficult to eradicate. Further refinements to the mouse model of AML are required to study AE-mediated RIT of AML. Other researchers in the AE field have also previously studied 111In- or 67Ga-labelled mAbs recognising MHC-II antigens or CD20 for RIT of B-cell lymphomas with promising results in vitro (Michel et al. 2003) as well as in vivo (Michel et al. 2005).

Somatostatin receptor subtype-2 (sst-2) which is overexpressed on neuroendocrine malignancies is another attractive target for radiotherapy with AEs. Octreotide is a stable octapeptide analog of somatostatin that preferentially binds with high affinity to sst-2. Capello et al. studied 111In-DTPA-octreotide for peptide receptor radionuclide therapy (PRRT) exploiting the AE emissions of 111In (Capello et al. 2003). Exposure of sst-2 positive CA20948 rat pancreatic tumour cells in vitro to 111In-DTPA-octreotide reduced their survival to 0%, while non-targeted 111In-DTPA had no inhibitory effects and unlabelled octreotide (1 μM) reduced survival by only 40%. In a subsequent in vivo study, Capello et al. showed that treatment of rats with CA20948 tumours with a single injection of 370 MBq or 3 injections of 111 MBq each of 111In-DTPA-octreotide yielded complete responses in small tumours and partial responses in larger tumours (Capello et al. 2005). 111In-DTPA-Octreotide has also been examined clinically for AE radiotherapy in patients with neuroendocrine malignancies (see Clinical Studies section).

Prostate-specific membrane antigen (PSMA) is a cell surface glycoprotein overexpressed on prostate cancer, but only found at low levels in other tissues such as the salivary gland, proximal small intestine and kidneys (Silver et al. 1997; Ghosh and Heston 2004). PSMA is therefore an attractive target for AE radiotherapy of prostate cancer. 161Tb is a radionuclide with a half-life of 6.89 days that emits a total of 5.1 keV of AE energy per decay with an average AE energy of 5.7 keV (Table 1). 161Tb also emits γ-photons at 49 keV and 75 keV suitable for SPECT imaging (Uusijärvi et al. 2006) and thus may have theranostic application for imaging and treatment of cancer. 161Tb-PSMA-617 was more potent for killing PSMA-positive PC-3 PIP tumour cells than PSMA-617 labelled with the β-emitter, 177Lu (177Lu-PSMA-617) when these cells were exposed in vitro to 0.05–10 MBq/mL (Müller et al. 2019). There was no effect on the viability of PSMA-negative PC-3 flu cells up to concentrations of 10 MBq/mL. In PC-3 PIP tumour-bearing athymic mice, 161Tb-PSMA-617 treatment achieved tumour growth delay, which was dependent on the amount administered. An administered amount of 10.0 MBq prolonged the time for tumour size to reach the end-point to 42 days vs. 28 days for 5.0 MBq and prolonged survival (65 days vs. 36 days, respectively). Tumour-inhibitory effects were more pronounced in mice treated with 161Tb-PSMA-617 than 177Lu-PSMA-617 when administered at 2.5 MBq, 5.0 MBq and 10.0 MBq.

Overcoming receptor heterogeneity in AE radiotherapy

A strength of AEs for cancer therapy is that their cytotoxicity is restricted mostly to cells that bind and internalise the targeting agent. There is no cross-fire effect but only a local cross-dose effect, which provides a high specificity for killing cancer cells. However, this poses a challenge since heterogeneity in target expression may allow some cells that are target-negative to escape the lethal effects of the AEs. In contrast longer range β-emitters, such as 177Lu and 90Y, have a cross-fire effect that is able to kill non-targeted cells within the range of the β-particles. The bystander effect of AEs may help to overcome the obstacle of receptor heterogeneity as AE-emitters from targeted irradiated cancer cells may still exert cytotoxic effects on non-targeted cells. The bystander effect describes the biological effects of radiation on cells that have not been directly irradiated (Marín et al. 2015). One of the first demonstrations of the bystander effect from AEs was reported by Xue et al. in nude mice inoculated with a mixture of untreated LS174T human colon cancer cells and cells loaded with lethal amounts of 125I-IUdR (Xue et al. 2002). Tumour growth was reduced significantly compared to inoculation of only untreated cells, yet the range of the AEs from 125I was subcellular (< 0.5 μm) and the absorbed dose deposited in the untreated cells from 125I-IUdR incorporated into the DNA of treated cells was < 10 cGy. The effects on tumour growth caused by the 125I-IUdR was therefore interpreted as a bystander effect. In another study, UVW human glioma cells and EJ138 human bladder carcinoma cells transfected with the noradrenaline transporter (NAT) gene that were exposed to medium from cells that accumulated 123I-MIBG showed a decrease in survival, indicating that mediators of the bystander effect were released into the medium from irradiated cells (Boyd et al. 2006).

Another strategy to address receptor heterogeneity is to design radioimmunoconjugates that recognise more than one receptor. We synthesised bispecific radioimmunoconjugates (bsRICs) that bind HER2 and EGFR (Razumienko et al. 2013, 2016) since upregulation of the EGFR and co-expression of EGFR and HER2 is a frequent mechanism of resistance to HER2-targeted therapies (Gallardo et al. 2012). These bsRICs were constructed by conjugating trastuzumab Fab fragments which bind HER2 through a flexible 24-mer PEG (PEG24) spacer to human EGF (hEGF). The immunoconjugates were modified with DTPA for labelling with 111In or DOTA for complex 177Lu (Razumienko et al. 2016). We also designed analogous bsRICs labelled with 64Cu for PET of tumours that co-express HER2 and EGFR (Kwon et al. 2017). In clonogenic assays, monospecific 177Lu- and 111In-trastuzumab Fab or EGF only killed tumour cells that expressed HER2 or EGFR, respectively, while the bsRICs were able to kill cells that expressed HER2 or EGFR or both receptors. These bsRICs were also more potent than the monospecific agents. 111In-labelled bsRICs were less effective than 177Lu-labelled bsRICs for treatment of MDA-MB-231/H2N tumours in mice that co-express HER2 and EGFR, but in this study, the same amount of radioactivity was administered for both bsRICs (11.1 MBq; 10 μg). This administered amount caused no observable normal tissue toxicity (NOAEL), which suggests that higher amounts of the 111In-labelled bsRICs could be safely administered to increase the therapeutic effects. It has been previously shown that RIT with AE-emitters yields more potent tumour growth-inhibitory effects in mice than β-emitters when administered at equitoxic but not necessarily equal amounts of radioactivity (Behr et al. 2000). Both 111In- and 177Lu-labelled bsRICs were also effective for treatment of TrR1 human breast cancer xenografts that are HER2 and EGFR-positive, but have acquired resistance to trastuzumab, but these tumours were less sensitive than MDA-MB-231/H2N tumour xenografts (Razumienko et al. 2016).

Modular nanotransporters (MNTs) represent an interesting and unique platform for targeting and internalising AE-emitters into cancer cells and delivering these radionuclides to the cell nucleus (Sobolev 2018). MNTs are recombinant multifunctional polypeptides that combine modules for receptor binding, internalisation, endosomal escape and nuclear importation. Slastnikova et al. reported that 125I-labelled EGFR-targeted MNTs (125I-N-succinimidyl-4-guanidinomethyl-3-[125I] iodobenzoate (SGMIB)-MNT) efficiently transported over 60% of the internalised radioactivity into the nucleus of EGFR-positive human epidermoid carcinoma A431 cells and human glioblastoma D247 MG cells after 1 h of incubation in vitro (Slastnikova et al. 2012). The cytotoxicity of the 125I-MNTs was dependent on EGFR density with greater killing observed for EGFR-overexpressing A431 cells than D247 cells with low EGFR expression.

Requirement for nuclear localisation of AE-emitters

Nuclear translocation may not be an absolute requirement for the cytotoxic effects of AE-emitters. Using 125I-labelled non-internalizing anti-CEA murine IgG1K 35A7 mAb, internalizing anti-HER2 trastuzumab mAb or anti-EGFR hybridoma murine m225 mAb and Tat cell penetrating peptide targeting the cell membrane, cytoplasm or the cell nucleus, respectively, Pouget et al. demonstrated that decreases in clonogenic survival of A-431 human vulvar squamous carcinoma and SKOV-3 human ovarian cancer cells were significantly higher for cell membrane than for cytoplasmic localisation (Pouget et al. 2008). Therefore, non-internalizing cell surface biomarkers such as carcinoembryonic antigen (CEA) may represent feasible targets for AE radiotherapies. Santoro et al. showed that 125I-35A7 anti-CEA mAbs significantly increased survival in nude mice bearing intraperitoneal EGFR-positive vulvar squamous cell carcinoma xenografts transfected to express CEA compared to treatment with unlabelled mAbs (59 days vs. 24 days) (Santoro et al. 2009). The effect was drastically reduced for 125I-labelled internalizing anti-EGFR mAbs and the corresponding unlabelled mAbs (77 days vs 76 days, respectively), however, this lower effectiveness could be explained by the catabolism of the internalised 125I-labelled mAbs with export of released 125I from the tumour cells (Santoro et al. 2009). Interestingly, Piron et al. showed the accumulation of unrepaired DNA DSBs over time after exposure to either 125I-labelled non-internalizing anti-CEA mAbs or internalizing anti-EGFR mAbs in human colorectal cancer HCT-116 cells (Piron et al. 2014). There was a lack of linear dose-effect relationship between the biological response observed and the absorbed dose deposited by the anti-CEA and anti-EGFR 125I-labelled mAbs, suggesting 125I may cause bystander effects on the cell-membrane (Piron et al. 2014).

Radiation nanomedicines

Nanoparticles labelled with AE-emitting radionuclides have also been studied for cancer treatment. Fonge et al. reported the construction of 111In-labelled BCMs modified with hEGF to target EGFR-positive breast cancer cells (Fonge et al. 2009). These 111In-hEGF-BCMs showed EGFR density-dependent cellular uptake and nuclear importation in a panel of human breast cancer cell lines (MDA-MB-468, MDA-MB-231 and MCF-7). Exposure of MDA-MB-468 cells with high EGFR expression to 111In-hEGF-BCMs reduced the clonogenic survival of these cells to 2.6%, but treatment of these cells with 111In-DTPA-hEGF was more effective, reducing the survival to 0.4%. Non-targeted 111In-BCMs were not effective for killing MDA-MB-468 cells, and 111In-hEGF-BCMs did not kill MDA-MB-231 or MCF-7 cells with intermediate or low EGFR expression, respectively. Hoang et al. reported the synthesis of BCMs incorporating polymers with DTPA to complex 111In, Fab fragments to bind HER2-positive breast cancer cells and NLS peptides to enable nuclear localisation (Hoang et al. 2012). The BCMs also incorporated methotrexate as a radiosensitiser, since this was previously shown to enhance the cytotoxicity of 111In-NLS-trastuzumab (Costantini et al. 2008a). Uptake of 111In-trastuzumab-Fab-BCMs in human breast cancer cells was dependent on the level of HER2 expression, and incorporation of NLS peptides enhanced the nuclear uptake of 111In. The clonogenic survival of SK-BR-3 cells, MDA-MB-361 and MDA-MB-231 cells with high, intermediate or low HER2 expression were reduced to approximately 23%, 45% and 77%, respectively, after exposure to 111In-NLS-trastuzumab-BCMs for 24 h in vitro.

Gold nanoparticles (AuNPs) have also been studied for AE radiotherapy. Song et al. reported the synthesis of 111In-labelled EGF-conjugated AuNPs (111In-EGF-AuNPs) (Song et al. 2016). Binding and internalisation of 111In-EGF-AuNPs by breast cancer cells was EGFR-dependent and was 12-fold higher for MDA-MB-468 than MCF-7 human breast cancer cells with high or low EGFR expression, respectively. Exposure of MDA-MB-468 cells for 4 h to 111In-EGF-AuNPs reduced the clonogenic survival to 17%, while less than 10% decreased survival was found for MCF-7 cells. A major limitation to systemic (i.v.) administration of radiolabelled AuNPs is high liver and spleen uptake mediated by interactions with the mononuclear phagocyte system (MPS), resulting in low tumour uptake (1–2%ID/g) in mouse tumour xenograft models (Chattopadhyay et al. 2012). Surface coating of nanoparticles with polyethylene glycol (PEG) can reduce MPS recognition. Song et al. constructed 111In-labelled PEGylated AuNPs modified with EGF to target EGFR for AE radiotherapy (Song et al. 2017). In mice with EGFR-positive MDA-MB-468 xenografts, liver uptake of i.v. injected of 111In-EGF-PEG-AuNPs was 3-fold lower than non-PEGylated 111In-EGF-AuNPs. Co-administration of an excess (15 μg) of EGF to block uptake by EGFR on hepatocytes improved tumour uptake from 2.8%ID/g to 3.9%ID/g. An alternative to systemic administration of radiolabelled AuNPs for cancer treatment is intratumoural (i.t.) injection, since this greatly minimises liver and spleen uptake due to retention of the AuNPs at the local injection site. We synthesised trastuzumab-modified AuNPs labelled with 111In for local treatment of HER2-positive breast cancer (Cai et al. 2016) (Fig. 9a). These 111In-trastuzumab-AuNPs were bound by HER2-positive SK-BR-3 or MDA-MB-361 human breast cancer cells and were internalised to a peri-nuclear location, likely mediated by a nuclear translocation sequence (NLS) in HER2 (Chen et al. 2005) (Fig. 9b). The emission of AEs by 111In caused lethal DNA DSBs in SK-BR-3 cells (Fig. 9c) reducing their CS by 3-fold. A single i.t. injection of 111In-AuNPs (10 MBq; 2.6 × 1012 AuNPs) arrested the growth of s.c. MDA-MB-361 tumours in mice without normal tissue toxicity (Fig. 9d). These results are promising for local injection of AuNPs labelled with AE-emitters for tumours that are accessible.

Fig. 9
figure 9

a 111In-trastuzumab-AuNPs are an example of an Auger electron (AE)-emitting radiation nanomedicine composed of gold nanoparticles (AuNPs; 30 nm) modified with 2 kDa polyethylene glycol (PEG) chains to stabilise the AuNPs and longer 5 kDa PEG chains conjugated to trastuzumab to bind HER2 or to DTPA to complex 111In. b Dark-field and fluorescence microscopy demonstrating peri-nuclear localisation (nucleus is stained blue with DAPI) of 111In-trastuzumab-AuNPs (yellow) in HER2-positive SK-BR-3 human breast cancer cells likely mediated by an endogenous nuclear localisation sequence (NLS) peptide in HER2. c DNA double-strand breaks (DSBs; bright foci) detected by immunofluorescence for γH2AX in the nucleus of SK-BR-3 cells exposed to 111In-trastuzumab-AuNPs mediated by emission of AEs by 111In. d Local intratumoural (i.t.) injection of 111In-trastuzumab-AuNPs (10 MBq) in athymic mice with subcutaneous HER2-positive MDA-MB-361 human breast cancer xenografts arrested tumour growth compared to untreated mice (left panel) with no change in body weight (right panel) indicating no generalised normal tissue toxicity (Cai et al. 2016)

Clinical studies

There have been only a few clinical studies of AEs for cancer therapy and some of these trials were conducted 20 years ago. Nonetheless, it is helpful to review the results of these trials to appreciate the potential clinical feasibility of AEs for cancer treatment. Macapinlac et al. conducted a pilot clinical trial of co-administration of 125I-IUdR (185 MBq) or 131I-IUdR (370 MBq) to evaluate dosimetry and safety in 4 patients with colorectal cancer metastatic to the liver using hepatic artery infusion (Macapinlac et al. 1996). No tumour responses were noted or expected at these low amounts of administered radioactivity. Images revealed no retention in the BM or in other normal tissues. No side effects or hematologic toxicity were observed. Tumour DNA samples showed higher incorporation of radioactivity compared to normal hepatocyte DNA. This study suggests that hepatic artery infusion may be a feasible route of delivery of AE radiopharmaceuticals for treatment of hepatic metastases, although studies at therapeutic doses would be required to assess the effectiveness of this approach. Rebischung et al. performed intrathecal injection of 125I-IUdR in a patient with advanced pancreatic cancer with resistant neoplastic meningitis (Rebischung et al. 2008). The patient was given 4 doses of methotrexate prior to and after administration of 1850 MBq of 125I-IUdR. The treatment yielded clinical improvement correlated with a dramatic decrease in cerebrospinal carbohydrate antigen 19.9 (CA19.9) from 202 U/mL on Day 0 to 9 U/mL on Day 26. No central nervous system toxicity was found. Unfortunately, the disease recurred and the patient ultimately died.

Krenning et al. reported a Phase 1 clinical trial of 111In-DTPA-octreotide in 30 patients with advanced sst-2 positive neuroendocrine malignancies (Krenning et al. 1999). Up to 14 doses of 111In-DTPA-octreotide at 6 to 7 GBq each were administered with at least a two week interval and a maximum cumulative radioactivity amount of 74 GBq. Among the 21 patients who received a total of > 20 GBq, 8 patients showed stable disease (SD) and 6 patients demonstrated a reduction in tumour size. No major side effects were noted up to 2 years post-treatment except for a transient drop in platelets and lymphocytes in some patients. Valkeman et al. reported a Phase 1 trial of 111In-DTPA-octreotide administered at intervals of 2 weeks to several months in 12 or more doses at 2 to 11 GBq to a total of 20–160 GBq in 50 patients with sst-2-positive tumours (Valkema et al. 2002). Among 40 evaluable patients, therapeutic benefit was achieved in 21 patients (52.5%) with stable disease in 14 patients, minor remissions in 6 patients and a partial remission in 1 patient. Mild hematopoietic toxicity was found in most patients, but myelodysplastic syndrome or leukemia developed in 3/6 patients who received a total dose > 100 GBq. Impairment in spermatogenesis was indicated in male patients who showed a decrease in serum inhibin B and concomitant increase of serum FSH. The radiation dose deposited in the kidneys from 111In-DTPA-octreotide was 0.45 mGy/MBq, corresponding to a total of 45 Gy for a total administered radioactivity of 100 GBq, which is twice the accepted limit for external beam radiation. However, none of these patients developed the sequelae expected for renal toxicity such as hypertension, proteinuria or any changes in serum creatinine or creatinine clearance, suggesting that this organ dosimetry did not predict renal toxicity from 111In-DTPA-octreotide. As mentioned above (see Radiation Dosimetry section) the regional distribution of 111In-DTPA-octreotide in the kidneys mainly in the renal tubules combined with the very short range of the AEs emitted by 111In, may protect the more radiation sensitive glomeruli (Konijnenberg et al. 2004). Limouris et al. infused 111In-DTPA-octreotide (average administered amount 6.3 GBq) via the hepatic artery in 17 patients with inoperable sst-2-positive liver metastases (Limouris et al. 2008). One patient achieved a complete response, while 8 patients exhibited a partial remission and 3 patients had stable disease. The median survival among the 12 responding patients was 32 months. Mild (grade 1) erythrocytopenia, leukocytopenia and thrombocytopenia were found in 3 patients.

Based on promising preclinical studies, our group conducted a Phase 1 clinical trial of 111In-DTPA-hEGF in 16 patients with metastatic EGFR-positive breast cancer administered 370–2220 MBq (0.25 mg) (Vallis et al. 2014) (Fig. 10). SPECT was used to assess the tumour and normal tissue uptake of 111In-DTPA-hEGF and to estimate radiation doses to normal organs. Toxicity was also evaluated. At these administered amounts, there were no hematopoietic, renal or hepatic toxicities. The estimated radiation dose to the whole body was 0.06 mGy/MBq, corresponding to 0.133 Gy at the maximum amount administered (2220 MBq). Following administration of 2200 MBq of 111In-DTPA-hEGF, the radiation dose to the kidneys (1.64 Gy) and liver (1.9 Gy) were within the radiation toxicity limit for these organs of 23 and 30 Gy, respectively, based on external beam radiotherapy (EBRT). However, some adverse effects were found that were related to the hEGF moiety. These included flushing, chills, nausea, and vomiting. One patient experienced grade 3 thrombocytopenia, but this was attributed to cancer metastasis to the BM rather than an adverse effect of 111In-DTPA-hEGF. No other patients experienced a serious adverse reaction. SPECT showed accumulation of 111In-DTPA-hEGF at known sites of breast cancer in 7/15 evaluable patients. Further dose-escalation of 111In-DTPA-hEGF is required to achieve a therapeutic effect, but the adverse effects associated with the hEGF moiety may require a higher SA than employed in this trial, in order to minimise the mass of hEGF injected. 111In-labelled anti-EGFR mAbs may be a promising alternative for AE-radiotherapy of EGFR-overexpressing breast cancer that would not cause these adverse effects (as discussed in the Preclinical Studies section). Our group is planning a Phase 1 clinical trial of 111In-NLS-trastuzumab to study its uptake in HER2-positive breast cancer by SPECT. Based on the results of this trial, we aim to conduct future therapeutic studies aimed at treatment of HER2-positive breast cancer with 111In-NLS-trastuzumab.

Fig. 10
figure 10

a Treatment of athymic mice with subcutaneous EGFR-positive MDA-MB-468 human breast cancer xenografts with 5 weekly amounts of 111In-DTPA-hEGF (cumulative dose, 92.5 MBq; 17 μg). Auger electron (AE) radiotherapy was effective compared to control mice treated with normal saline, but the growth of smaller, non-established tumours (right panel) was more strongly inhibited than larger tumours (left panel) (Chen et al. 2003). b SPECT and corresponding CT images of two patients at 24 h after injection of 111In-DTPA-hEGF in a Phase 1 clinical trial, demonstrating uptake into a recurrent primary breast cancer (left panels) or a lung metastasis (right panels) (Vallis et al. 2014)

Li et al. reported a Phase 2 clinical trial of adjuvant RIT with 125I-labelled murine anti-EGFR mAb 425 in 192 patients with glioblastoma multiforme (GBM) (Li et al. 2010). Up to 3 weekly intravenous injections of 125I-mAb 425 (1.8 GBq each) were administered with a maximum cumulative radioactivity amount of 5.4 GBq. Among these 192 patients, 132 patients received RIT alone, and 60 patients received RIT and temozolomide (TMZ) chemotherapy. The median overall survival of 97 patients who received RIT alone was 14.5 months (range 12.1–16.7 months), and for 51 patients who received RIT and TMZ, the overall median survival was 20.4 months (range 14.9–25.8 months). Both treatment arms resulted in significantly improved overall survival compared to a historical control group of 39 patients receiving standard-of-care treatment, who had a median survival of 10.2 months (range 8.4–12.0 months). No grade 3 or 4 toxicities were observed for either of the RIT treatment groups, with only 7 of the 192 patients experiencing acute side effects (transient flushing, grade 1 nausea, hypotension and skin irritation). Four patients developed human anti-mouse antibodies (HAMA) preventing further administration of 125I-mAb 425, which was a murine mAb. The development of humanised or chimeric (e.g. cetuximab) and fully human anti-EGFR mAbs (e.g. panitumumab) in recent years should obviate this immunogenicity issue.


AEs have very attractive properties for cancer therapy since their nanometre-micrometre range results in high LET that is potent for causing lethal damage in cancer cells. Biomolecules (mAbs and peptides), nucleosides and nanoparticles have been labelled with AE-emitting radionuclides (e.g. 111In, 67Ga, 99mTc, 123I or 125I) and studied for cancer treatment. Several concepts have emerged. Firstly, AEs are especially lethal to cancer cells when emitted in close proximity to the cell nucleus, and particularly if the AE-emitting radiotherapeutic agent is incorporated directly into DNA (e.g. 125I-IUdR). Strategies that promote the delivery of AE-emitters to the nucleus by conjugating internalising mAbs to NLS peptides (e.g. 111In-NLS-trastuzumab), or targeting a receptor that harbours an endogenous NLS to enable nuclear uptake (e.g. EGFR or HER2) amplifies the lethal DNA-damaging effects of AEs. Nonetheless, nuclear localisation is not an absolute requirement and AEs can also kill targeted cancer cells by damaging the cell membrane, or non-targeted cells by a local cross-dose effect or a longer range bystander effect. Numerous studies have shown that AEs can kill cancer cells in vitro in clonogenic assays by inflicting lethal DNA damage (e.g. DSBs) detected by immunofluorescence for γH2AX foci in the nucleus or by damaging the cell membrane. Preclinical studies of AE-emitting radiotherapeutic agents in vivo in mouse tumour xenograft models have further demonstrated that cancer treatment using AEs is feasible. Strong tumour growth inhibition has been achieved with minimal toxicity to normal tissues at the amounts administered due to the mostly restricted cytotoxicity of AEs towards cancer cells that bind these radiotherapeutic agents. A limited number of clinical studies of AEs for cancer therapy have been performed, and these mainly evaluated the tumour and normal tissue localisation of the radiotherapeutic agents, estimated normal organ dosimetry and assessed normal tissue toxicity at relatively low administered amounts. Nonetheless, some studies have shown promising results for treatment of cancer with AE-emitting radiotherapeutic agents (e.g. 125I-IUdR, 111In-DTPA-octreotide or 125I-mAb 425), achieving tumour remissions or improved survival in patients. The recent introduction of a large armamentarium of biologically-targeted therapies for cancer, especially humanised and fully human mAbs creates many new opportunities to design novel AE-emitting radiotherapeutic agents for cancer treatment. In only a few years, we will celebrate the 100th anniversary of the publication by Pierre Auger (Auger 1923) on the discovery of these electrons that bear his name. Pierre Auger did not conceive of the application of AEs for targeted cancer treatment, but this is a tremendously exciting future that we and many other scientists in this field envision.

Availability of data and materials

Not applicable.













Auger electrons


Acute myeloid leukemia


Ataxia telangiectasia mutated


Area under the curve


Gold nanoparticles


Breast cancer


Block copolymer micelles


Bone marrow


Bispecific radioimmunoconjugates




Chinese Hamster Ovary


Confidence interval


Clonogenic survival




1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid


Double strand breaks


Diethylenetriaminepentaacetic acid


External beam radiotherapy


Electron capture


Ethylene diamine N,N′-diacetic acid


Epidermal growth factor receptor


Glioblastoma multiforme


Human anti-mouse antibodies


Human epidermal growth factor


Human epidermal growth factor receptor 2










International Atomic Energy Agency


Internal conversion


125I- or 123I-5-iodo-2-deoxyuridine


Linear energy transfer


Leukemic stem cells


Monoclonal antibody


Metal-chelating polymers


Multidrug resistance


Medical Internal Radiation Dose


Modular nanotransporters


Mononuclear phagocyte system


Nuclear localisation sequence


No observable adverse effects


1,4,7-triazacyclononane-1,4,7-triacetic acid


NOD-Rag1null IL2rgnull, NOD rag gamma


Non-uniform rational B-splines






Polyethylene glycol

PEG24 :

24 mer PEG


Pulsed-field gel electrophoresis


Peptide receptor radionuclide therapy




Radiotracer Biodistribution Template


Relative biological effectiveness






Reactive oxygen species

rS :

Source region

rT :

Target region


Specific activity


Single cell gel electrophoresis assay


Stable disease


Single-photon emission computed tomography


Somatostatin receptor subtype-2

Tb :

Biological half life

TD :

Dose-integration period

Te :

Effective half life



Tp :

Physical half-life


White blood cell


  • 65-Terbium-161. National Nuclear Data Centre. (2011).

  • Abuqbeitah M, Demir M, Çavdar İ, Tanyildizi H, Yeyin N, Uslu-Beşli L, Kabasakal L, Işıkcı Nİ, Sönmezoğlu K. Red bone marrow dose estimation using several internal dosimetry models for prospective dosimetry-oriented radioiodine therapy. Radiat Environ Biophys. 2018;57(4):395–404.

    CAS  PubMed  Article  Google Scholar 

  • Aghevlian S, Lu Y, Winnik MA, Hedley DW, Reilly RM. Panitumumab modified with metal-chelating polymers (MCP) complexed to 111In and 177Lu—an EGFR-targeted theranostic for pancreatic cancer. Mol Pharm. 2018;15(3):1150–9.

    CAS  PubMed  Article  Google Scholar 

  • Andersson M, Johansson L, Eckerman K, Mattsson S. IDAC-Dose 2.1, an internal dosimetry program for diagnostic nuclear medicine based on the ICRP adult reference voxel phantoms. EJNMMI Res. 2017;7(1):88.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  • Areberg J, Björkman S, Einarsson L, Frankenberg B, Lundqvist H, Mattsson S, Norrgren K, Scheike O, Wallin R. Gamma camera imaging of platinum in tumours and tissues of patients after administration of 191Pt-cisplatin. Acta Oncol. 1999;38(2):221–8.

    CAS  PubMed  Article  Google Scholar 

  • Areberg J, Johnsson A, Wennerberg J. In vitro toxicity of 191Pt-labeled cisplatin to a human cervical carcinoma cell line (ME-180). Int J Radiat Oncol Biol Phys. 2000;46(5):1275–80.

    CAS  PubMed  Article  Google Scholar 

  • Areberg J, Wennerberg J, Johnsson A, Norrgren K, Mattsson S. Antitumor effect of radioactive cisplatin (191Pt) on nude mice. Int J Radiat Oncol Biol Phys. 2001;49(3):827–32.

    CAS  PubMed  Article  Google Scholar 

  • Auger P. Sur les rayons β secondaires produits dans un gaz par des rayons X. CR Acad Sci. 1923;177:169.

    CAS  Google Scholar 

  • Auger P. L’effet photoélectrique compose. Ann Phys. 1926;10(6):183–253.

    Article  Google Scholar 

  • Auger P. The Auger effect. Surf Sci. 1975;48(1):1–8.

    CAS  Article  Google Scholar 

  • Balagurumoorthy P, Xu X, Wang K, Adelstein SJ, Kassis AI. Effect of distance between decaying 125I and DNA on Auger-electron induced double-strand break yield. Int J of Radiat Biol. 2012;88(12):998–1008.

    CAS  Article  Google Scholar 

  • Bavelaar BM, Lee BQ, Gill MR, Falzone N, Vallis KA. Subcellular targeting of theranostic radionuclides. Front Pharmacol. 2018;9.

  • Beckmann MW, Scharl A, Rosinsky BJ, Holt JA. Breaks in DNA accompany estrogen-receptor-mediated cytotoxicity from 16α [125I] iodo-17β-estradiol. J of Cancer Res Clin Oncol. 1993;119(4):207–14.

    CAS  Article  Google Scholar 

  • Behr TM, Béhé M, Löhr M, Sgouros G, Angerstein C, Wehrmann E, Nebendahl K, Becker W. Therapeutic advantages of Auger electron- over beta-emitting radiometals or radioiodine when conjugated to internalizing antibodies. Eur J Nucl Med. 2000;27(7):753–65.

    CAS  PubMed  Article  Google Scholar 

  • Berger MJ, Coursey JS, Zucker MA, Chang J. ESTAR, PSTAR, and ASTAR: computer programs for calculating stopping-power and range tables for electrons, protons, and helium ions (version 1.2.3). Natl Inst Stand Technol. 2005;

  • Bergstrom D, Leyton JV, Zereshkian A, Chan C, Cai Z, Reilly RM. Paradoxical effects of Auger electron-emitting 111In-DTPA-NLS-CSL360 radioimmunoconjugates on hCD45+ cells in the bone marrow and spleen of leukemia-engrafted NOD/SCID or NRG mice. Nucl Med Biol. 2016;43(10):635–41.

    CAS  PubMed  Article  Google Scholar 

  • Bhattathiri NV, Bindu L, Remani P, Chandralekha B, Nair KM. Radiation-induced acute immediate nuclear abnormalities in oral cancer cells: serial cytologic evaluation. Acta Cytol. 1998;42(5):1084–90.

    CAS  PubMed  Article  Google Scholar 

  • Bodnar EN, Dikiy MP, Medvedeva EP. Photonuclear production and antitumor effect of radioactive cisplatin (195mPt). J Radioanal Nucl Chem. 2015;305(1):133–8.

    CAS  Article  Google Scholar 

  • Bolch WE, Bouchet LG, Robertson JS, Wessels BW, Siegel JA, Howell RW, Erdi AK, Aydogan B, Costes S, Watson EE. MIRD committee. MIRD pamphlet no. 17: the dosimetry of nonuniform activity distributions—radionuclide S values at the voxel level. J Nucl Med. 1999;40(1):11S–36S.

    CAS  PubMed  Google Scholar 

  • Bolch WE, Eckerman KF, Sgouros G, Thomas SR. MIRD pamphlet no. 21: a generalized schema for radiopharmaceutical dosimetry—standardization of nomenclature. J Nucl Med. 2009;50(3):477–84.

    CAS  PubMed  Article  Google Scholar 

  • Bonnet D, Bhatia M, Wang JC, Kapp U, Dick JE. Cytokine treatment or accessory cells are required to initiate engraftment of purified primitive human hematopoietic cells transplanted at limiting doses into NOD/SCID mice. Bone Marrow Transplant. 1999;23(3):203.

    CAS  PubMed  Article  Google Scholar 

  • Bouchet LG, Bolch WE, Blanco HP, Wessels BW, Siegel JA, Rajon DA, Clairand I, Sgouros G. MIRD pamphlet no. 19: absorbed fractions and radionuclide S values for six age-dependent multiregion models of the kidney. J Nucl Med. 2003;44(7):1113–47.

    PubMed  Google Scholar 

  • Bouchet LG, Bolch WE, Weber DA, Atkins HL, Poston JW. MIRD pamphlet no. 15: radionuclide S values in a revised dosimetric model of the adult head and brain. J Nucl Med. 1999;40:62S–71S.

    CAS  PubMed  Google Scholar 

  • Boyd M, Ross SC, Dorrens J, Fullerton NE, Tan KW, Zalutsky MR, Mairs RJ. Radiation-induced biologic bystander effect elicited in vitro by targeted radiopharmaceuticals labeled with α-, β-, and Auger electron–emitting radionuclides. J Nucl Med. 2006;47(6):1007–15.

    CAS  PubMed  Google Scholar 

  • Brom M, Joosten L, Oyen WJ, Gotthardt M, Boerman OC. Improved labelling of DTPA-and DOTA-conjugated peptides and antibodies with 111In in HEPES and MES buffer. EJNMMI Res. 2012;2(1):4.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  • Burdak-Rothkamm S, Prise KM. New molecular targets in radiotherapy: DNA damage signalling and repair in targeted and non-targeted cells. Eur J Pharmacol. 2009;625(1–3):151–5.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Cai Z, Chattopadhyay N, Yang K, Kwon YL, Yook S, Pignol JP, Reilly RM. 111In-labeled trastuzumab-modified gold nanoparticles are cytotoxic in vitro to HER2-positive breast cancer cells and arrest tumor growth in vivo in athymic mice after intratumoral injection. Nucl Med Biol. 2016;43(12):818–26.

    CAS  PubMed  Article  Google Scholar 

  • Cai Z, Chen Z, Bailey KE, Scollard DA, Reilly RM, Vallis KA. Relationship between induction of phosphorylated H2AX and survival in breast cancer cells exposed to 111In-DTPA-hEGF. J Nucl Med. 2008;49(8):1353–61.

    CAS  PubMed  Article  Google Scholar 

  • Cai Z, Kwon YL, Reilly RM. Monte Carlo N-particle (MCNP) modeling of the cellular dosimetry of 64Cu: comparison with MIRDcell S values and implications for studies of its cytotoxic effects. J Nucl Med. 2017;58(2):339–45.

    CAS  PubMed  Article  Google Scholar 

  • Cai Z, Pignol JP, Chan C, Reilly RM. Cellular dosimetry of in-111 using Monte Carlo N-particle computer code: comparison with analytic methods and correlation with in vitro cytotoxicity. J Nucl Med. 2010;51:462–70.

    PubMed  Article  Google Scholar 

  • Cai Z, Vallis KA, Reilly RM. Computational analysis of the number, area and density of γ-H2AX foci in breast cancer cells exposed to 111In-DTPA-hEGF or γ-rays using image-J software. Int J Radiat Biol. 2009;85(3):262–71.

    CAS  PubMed  Article  Google Scholar 

  • Capello A, Krenning E, Bernard B, Reubi JC, Breeman W, de Jong M. 111In-labelled somatostatin analogues in a rat tumour model: somatostatin receptor status and effects of peptide receptor radionuclide therapy. Eur J Nucl Med Mol Imaging. 2005;32(11):1288–95.

    CAS  PubMed  Article  Google Scholar 

  • Capello A, Krenning EP, Breeman WA, Bernard BF, de Jong M. Peptide receptor radionuclide therapy in vitro using [111In-DTPA0] octreotide. J Nucl Med. 2003;44(1):98–104.

    CAS  PubMed  Google Scholar 

  • Carr BI. Hepatic arterial 90Yttrium glass microspheres (Therasphere) for unresectable hepatocellular carcinoma: interim safety and survival data on 65 patients. Liver Transpl. 2004;10(S2):S107–10.

    PubMed  Article  Google Scholar 

  • Carrillo-Cázares TA, Torres-García E. Monte Carlo mitochondrial dosimetry and microdosimetry of 131I. Radiat Prot Dosim. 2012;153(4):411–6.

    Article  CAS  Google Scholar 

  • Chalkia MT, Stefanoyiannis AP, Chatziioannou SN, Round WH, Efstathopoulos EP, Nikiforidis GC. Patient-specific dosimetry in peptide receptor radionuclide therapy: a clinical review. Australas Phys Eng Sci Med. 2015;38(1):7–22.

    CAS  PubMed  Article  Google Scholar 

  • Chan C, Cai Z, Reilly RM. Trastuzumab labeled to high specific activity with 111In by conjugation to G4 PAMAM dendrimers derivatized with multiple DTPA chelators exhibits increased cytotoxic potency on HER2-positive breast cancer cells. Pharm Res. 2013;30(8):1999–2009.

    CAS  PubMed  Article  Google Scholar 

  • Chan PC, Lisco E, Lisco H, Adelstein SJ. The radiotoxicity of Iodine-125 in mammalian cells: II. A comparative study on cell survival and cytogenetic responses to 125IUdR, 131IUdR, and 3HTdR. J Radiat Res. 1976;67:332–43.

    CAS  Article  Google Scholar 

  • Charlton DE. The range of high LET effects from 125I decays. J Radiat Res. 1986;107(2):163–71.

    CAS  Article  Google Scholar 

  • Chattopadhyay N, Fonge H, Cai Z, Scollard D, Lechtman E, Done SJ, Pignol JP, Reilly RM. Role of antibody-mediated tumor targeting and route of administration in nanoparticle tumor accumulation in vivo. Mol Pharm. 2012;9(8):2168–79.

    CAS  PubMed  Article  Google Scholar 

  • Chen P, Cameron R, Wang J, Vallis KA, Reilly RM. Antitumor effects and normal tissue toxicity of 111In-labeled epidermal growth factor administered to athymic mice bearing epidermal growth factor receptor-positive human breast cancer xenografts. J Nucl Med. 2003;44(9):1469–78.

    CAS  PubMed  Google Scholar 

  • Chen P, Wang J, Hope K, Jin L, Dick J, Cameron R, Brandwein J, Minden M, Reilly RM. Nuclear localizing sequences promote nuclear translocation and enhance the radiotoxicity of the anti-CD33 monoclonal antibody HuM195 labeled with 111In in human myeloid leukemia cells. J Nucl Med. 2006;47(5):827–36.

    CAS  PubMed  Google Scholar 

  • Chen QQ, Chen XY, Jiang YY, Jing LI. Identification of novel nuclear localization signal within the ErbB-2 protein. Cell Res. 2005;15(7):504.

    CAS  PubMed  Article  Google Scholar 

  • Chen Y, Vastenhouw B, Wu C, Goorden MC, Beekman FJ. Optimized image acquisition for dopamine transporter imaging with ultra-high resolution clinical pinhole SPECT. Phys Med Biol. 2018;63(22).

    Article  Google Scholar 

  • Chin BB, Kronauge JF, Femia FJ, Chen J, Maresca KP, Hillier S, Petry NA, James OG, Oldan JD, Armor T, Stubbs JB. Phase-1 clinical trial results of high-specific-activity carrier-free 123I-iobenguane. J Nucl Med. 2014;55(5):765–71.

    CAS  PubMed  Article  Google Scholar 

  • Choppin G, Liljenzin JO, Rydberg J. Chapter 4 - unstable nuclei and radioactive decay. In: Choppin G, Liljenzin JO, Rydberg J, editors. Radiochemistry and nuclear chemistry. 3rd ed. Woburn: Butterworth-Heinemann; 2002. p. 58–93.

    Chapter  Google Scholar 

  • Costantini DL, Bateman K, McLarty K, Vallis KA, Reilly RM. Trastuzumab-resistant breast cancer cells remain sensitive to the auger electron–emitting radiotherapeutic agent 111In-NLS-trastuzumab and are radiosensitized by methotrexate. J Nucl Med. 2008a;49(9):1498–505.

    CAS  PubMed  Article  Google Scholar 

  • Costantini DL, Chan C, Cai Z, Vallis KA, Reilly RM. 111In-labeled trastuzumab (Herceptin) modified with nuclear localization sequences (NLS): an Auger electron-emitting radiotherapeutic agent for HER2/neu-amplified breast cancer. J Nucl Med. 2007;48(8):1357–68.

    CAS  PubMed  Article  Google Scholar 

  • Costantini DL, Hu M, Reilly RM. Update: peptide motifs for insertion of radiolabeled biomolecules into cells and routing to the nucleus for cancer imaging or radiotherapeutic applications. Cancer Biother Radiopharm. 2008b;23(1):3–24.

    CAS  PubMed  Article  Google Scholar 

  • Costantini DL, McLarty K, Lee H, Done SJ, Vallis KA, Reilly RM. Antitumor effects and normal-tissue toxicity of 111In-nuclear localization sequence-trastuzumab in athymic mice bearing HER-positive human breast cancer xenografts. J Nucl Med. 2010;51(7):1084–91.

    CAS  PubMed  Article  Google Scholar 

  • Desbois N, Gardette M, Papon J, Labarre P, Maisonial A, Auzeloux P, Lartigue C, Bouchon B, Debiton E, Blache Y, Chavignon O. Design, synthesis and preliminary biological evaluation of acridine compounds as potential agents for a combined targeted chemo-radionuclide therapy approach to melanoma. Bioorg Med Chem. 2008;16(16):7671–90.

    CAS  PubMed  Article  Google Scholar 

  • Dewaraja YK, Frey EC, Sgouros G, Brill AB, Roberson P, Zanzonico PB, Ljungberg M. MIRD pamphlet no. 23: quantitative SPECT for patient-specific 3-dimensional dosimetry in internal radionuclide therapy. J Nucl Med. 2012;53(8):1310–25.

    CAS  PubMed  Article  Google Scholar 

  • Di Maria S, Belchior A, Romanets Y, Paulo A, Vaz P. Monte Carlo dose distribution calculation at nuclear level for Auger-emitting radionuclide energies. Appl Radiat Isot. 2018;135:72–7.

    PubMed  Article  CAS  Google Scholar 

  • Dong Y, Gao Y, Liu W, Gao T, Zheng Y, Sanche L. Clustered DNA damage induced by 2–20 eV electrons and transient anions: general mechanism and correlation to cell death. J Phys Chem Lett. 2019;10(11):2985–90.

    CAS  PubMed  Article  Google Scholar 

  • Dykiy MP, Dovbnya AN, Lyashko YV, Medvedeva EP, Medvedev DV, Uvarov VL. Photonuclear production of 193m, 195mPt and synthesis of radioactive cisplatin. J Labelled Comp Radiopharm. 2007;50:480–2.

    CAS  Article  Google Scholar 

  • Eckerman KF, Endo A. MIRD: Radionuclide Data and Decay Schemes. 2nd ed. Reston, VA: Society of Nuclear Medicine; 2008. p. 117.

    Google Scholar 

  • Elmroth K, Stenerlöw B. DNA-incorporated 125I induces more than one double-strand break per decay in mammalian cells. J Radiat Res. 2005;163(4):369–73.

    CAS  Article  Google Scholar 

  • Eriksson D, Stigbrand T. Radiation-induced cell death mechanisms. Tumor Biol. 2010;31(4):363–72.

    Article  Google Scholar 

  • Falzone N, Ackerman NL, de la Fuente RL, Bernal MA, Liu X, Peeters SG, Soto MS, Corroyer-Dulmont A, Bernaudin M, Grimoin E, Touzani O. Dosimetric evaluation of radionuclides for VCAM-1-targeted radionuclide therapy of early brain metastases. Theranostics. 2018;8(1):292.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Falzone N, Lee BQ, Able S, Malcolm J, Terry S, Alayed Y, Vallis KA. Targeting micrometastases: the effect of heterogeneous radionuclide distribution on tumor control probability. J Nucl Med. 2019;60(2):250–8.

    CAS  PubMed Central  Article  Google Scholar 

  • Falzone N, Lee BQ, Fernández-Varea JM, Kartsonaki C, Stuchbery AE, Kibédi T, Vallis KA. Absorbed dose evaluation of Auger electron-emitting radionuclides: impact of input decay spectra on dose point kernels and S-values. Phys Med Biol. 2017;62(6):2239.

    CAS  PubMed  Article  Google Scholar 

  • Faraggi M, Gardin I, de Labriolle-Vaylet C, Moretti JL, Bok BD. The influence of tracer localization on the electron dose rate delivered to the cell nucleus. J Nucl Med. 1994;35(1):113–9.

    CAS  PubMed  Google Scholar 

  • Fasih A, Fonge H, Cai Z, Leyton JV, Tikhomirov I, Done SJ, Reilly RM. 111In-Bn-DTPA-nimotuzumab with/without modification with nuclear translocation sequence (NLS) peptides: an Auger electron-emitting radioimmunotherapeutic agent for EGFR-positive and trastuzumab (Herceptin)-resistant breast cancer. Breast Cancer Res Treat. 2012;135(1):189–200.

    CAS  PubMed  Article  Google Scholar 

  • Fisher DR, Shen S, Meredith RF. MIRD dose estimate report no. 20: radiation absorbed-dose estimates for 111In-and 90Y-ibritumomab tiuxetan. J Nucl Med. 2009;50(4):644–52.

    CAS  PubMed  Article  Google Scholar 

  • Fonge H, Lee H, Reilly RM, Allen C. Multifunctional block copolymer micelles for the delivery of 111In to EGFR-positive breast cancer cells for targeted Auger electron radiotherapy. Mol Pharm. 2009;7(1):177–86.

    Article  CAS  Google Scholar 

  • Fraker PJ, Speck JC Jr. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1, 3, 4, 6-tetrachloro-3a, 6a-diphenylglycoluril. Biochem Biophys Res Commun. 1978;80(4):849–57.

    CAS  PubMed  Article  Google Scholar 

  • Freudenberg R, Runge R, Maucksch U, Berger V, Kotzerke J. On the dose calculation at the cellular level and its implications for the RBE of 99mTc and 123I. Med Phys. 2014;41(6Part1):062503.

    CAS  PubMed  Article  Google Scholar 

  • Gallardo A, Lerma E, Escuin D, Tibau A, Munoz J, Ojeda B, Barnadas A, Adrover E, Sánchez-Tejada L, Giner D, Ortiz-Martínez F. Increased signalling of EGFR and IGF1R, and deregulation of PTEN/PI3K/Akt pathway are related with trastuzumab resistance in HER2 breast carcinomas. Br J Cancer. 2012;106(8):1367.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Gao C, Leyton JV, Schimmer AD, Minden M, Reilly RM. Auger electron-emitting 111In-DTPA-NLS-CSL360 radioimmunoconjugates are cytotoxic to human acute myeloid leukemia (AML) cells displaying the CD123+/CD131− phenotype of leukemia stem cells. Int J Rad Appl Instrum A. 2016;110:1–7.

    CAS  Google Scholar 

  • Gardette M, Viallard C, Paillas S, Guerquin-Kern JL, Papon J, Moins N, Labarre P, Desbois N, Wong-Wah-Chung P, Palle S, Wu TD. Evaluation of two 125I-radiolabeled acridine derivatives for Auger-electron radionuclide therapy of melanoma. Investig New Drugs. 2014;32(4):587–97.

    CAS  Article  Google Scholar 

  • Ghosh A, Heston WD. Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancer. J Cell Biochem. 2004;91(3):528–39.

    CAS  PubMed  Article  Google Scholar 

  • Goddu SM, Howell RW, Bouchet LG, Bolch WE, Rao D. MIRD Cellular S Values. Reston, VA: Society of Nuclear Medicine; 1997.

    Google Scholar 

  • Goddu SM, Narra VR, Harapanhalli RS, Howell RW, Rao DV. Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo: comparison with other radioprotectors and evidence for indirect action of Auger electrons. Acta Oncol. 1996;35(7):901–7.

    CAS  PubMed  Article  Google Scholar 

  • Gorodetsky R, Levy-Agababa F, Mou X, Vexler AM. Combination of cisplatin and radiation in cell culture: effect of duration of exposure to drug and timing of irradiation. Int J Cancer. 1998;75(4):635–42.

    CAS  PubMed  Article  Google Scholar 

  • Haefliger P, Agorastos N, Renard A, Giambonini-Brugnoli G, Marty C, Alberto R. Cell uptake and radiotoxicity studies of an nuclear localization signal peptide− intercalator conjugate labeled with [99mTc (CO)3]+. Bioconjug Chem. 2005;16(3):582–7.

    CAS  PubMed  Article  Google Scholar 

  • Haines GA, Hendry JH, Daniel CP, Morris ID. Increased levels of comet-detected spermatozoa DNA damage following in vivo isotopic-or X-irradiation of spermatogonia. Mutat Res Genet Toxicol Environ Mutagen. 2001;495(1–2):21–32.

    CAS  Article  Google Scholar 

  • Hoang B, Ekdawi SN, Reilly RM, Allen C. Active targeting of block copolymer micelles with trastuzumab fab fragments and nuclear localization signal leads to increased tumor uptake and nuclear localization in HER2-overexpressing xenografts. Mol Pharm. 2013;10(11):4229–41.

    CAS  PubMed  Article  Google Scholar 

  • Hoang B, Reilly RM, Allen C. Block copolymer micelles target auger electron radiotherapy to the nucleus of HER2-positive breast cancer cells. Biomacromolecules. 2012;13(2):455–65.

    CAS  PubMed  Article  Google Scholar 

  • Howell RW. Radiation spectra for Auger electron emitting radionuclides: report no. 2 of AAPM nuclear medicine task group no. 6. Med Phys. 1992;19(6):1371–83.

    CAS  PubMed  Article  Google Scholar 

  • Hoyes KP, Lord BI, McCann C, Hendry JH, Morris ID. Transgenerational effects of preconception paternal contamination with 55Fe. Radiat Res. 2001;156(5):488–94.

    CAS  PubMed  Article  Google Scholar 

  • Hubbell JH, Seltzer SM. Tables of X-ray mass attenuation coefficients and mass energy-absorption coefficients (version 1.4). Natl Inst Stand Technol. 2004;

  • IAEA Radiotracer Biodistribution Template (RaBiT) (n.d.). Available at:

  • Ickenstein LM, Edwards K, Sjöberg S, Carlsson J, Gedda L. A novel 125I-labeled daunorubicin derivative for radionuclide-based cancer therapy. Nucl Med Biol. 2006;33(6):773–83.

    CAS  PubMed  Article  Google Scholar 

  • IDAC-Dose2.1 (n.d.). Avalable at:

  • Iliakis GE, Cicilioni O, Metzger L. Measurement of DNA double-strand breaks in CHO cells at various stages of the cell cycle using pulsed field gel electrophoresis: calibration by means of 125I decay. Int J Radiat Biol. 1991;59(2):343–57.

    CAS  PubMed  Article  Google Scholar 

  • Imstepf S, Pierroz V, Raposinho P, Bauwens M, Felber M, Fox T, Shapiro AB, Freudenberg R, Fernandes C, Gama S, Gasser G. Nuclear targeting with an Auger electron emitter potentiates the action of a widely used antineoplastic drug. Bioconjug Chem. 2015;26(12):2397–407.

    CAS  PubMed  Article  Google Scholar 

  • Intemann RL, Pollock F. K-electron ejection accompanying nuclear K capture. Phys Rev. 1967;157(1):41.

    CAS  Article  Google Scholar 

  • Ismail IH, Nyström S, Nygren J, Hammarsten O. Activation of ataxia telangiectasia mutated by DNA strand break-inducing agents correlates closely with the number of DNA double strand breaks. J Biol Chem. 2005;280(6):4649–55.

    CAS  PubMed  Article  Google Scholar 

  • Jordan CT, Upchurch D, Szilvassy SJ, Guzman ML, Howard DS, Pettigrew AL, Meyerrose T, Rossi R, Grimes B, Rizzieri DA, Luger SM. The interleukin-3 receptor alpha chain is a unique marker for human acute myelogenous leukemia stem cells. Leukemia. 2000;14(10):1777.

    CAS  PubMed  Article  Google Scholar 

  • Karamychev VN, Reed MW, Neumann RD, Panyutin IG. Distribution of DNA strand breaks produced by iodine-123 and indium-111 in synthetic oligodeoxynucleotides. Acta Oncol. 2000;39(6):687–92.

    CAS  PubMed  Article  Google Scholar 

  • Kassis AI, Fayad F, Kinsey BM, Sastry KS, Taube RA, Adelstein SJ. Radiotoxicity of 125I in mammalian cells. Radiat Res. 1987;111(2):305–18.

    CAS  PubMed  Article  Google Scholar 

  • Kersemans V, Cornelissen B, Minden MD, Brandwein J, Reilly RM. Drug-resistant AML cells and primary AML specimens are killed by 111In-anti-CD33 monoclonal antibodies modified with nuclear localizing peptide sequences. J Nucl Med. 2008;49(9):1546–54.

    CAS  PubMed  Article  Google Scholar 

  • Kesner AL, Bodei L. Modern radiopharmaceutical dosimetry should include robust biodistribution reporting. J Nucl Med. 2018;59(10):1507.

    PubMed  Article  Google Scholar 

  • Kesner AL, Poli GL, Beykan S, Lassmann M. The IAEA radiotracer biodistribution template–a community resource for supporting the standardization and reporting of radionuclide pre-dosimetry data. Phys Medica. 2017;44:83–5.

    Article  Google Scholar 

  • Kirkby C, Ghasroddashti E. Targeting mitochondria in cancer cells using gold nanoparticle enhanced radiotherapy: a Monte Carlo study. Med Phys. 2015;42(2):1119–28.

    CAS  PubMed  Article  Google Scholar 

  • Kirschner AS, Ice RD, Beierwaltes WH. Radiation-dosimetry of 131I-19-iodocholesterol - pitfalls of using tissue concentration data - reply. J Nucl Med. 1975;16:248–9.

    CAS  Google Scholar 

  • Konijnenberg MW, Bijster M, Krenning EP, De Jong M. A stylized computational model of the rat for organ dosimetry in support of preclinical evaluations of peptide receptor radionuclide therapy with 90Y, 111In, or 177Lu. J Nucl Med. 2004;45(7):1260–9.

    CAS  PubMed  Google Scholar 

  • Krenning EP, De Jong M, Kooij PP, Breeman WA, Bakker WH, De Herder WW, Van Eijck CH, Kwekkeboom DJ, Jamar F, Pauwels S, Valkema R. Radiolabelled somatostatin analogue (s) for peptide receptor scintigraphy and radionuclide therapy. Ann Oncol. 1999;10(Suppl 2):S23–9.

    PubMed  Article  Google Scholar 

  • Kriehuber R, Kadenbach K, Schultz F, Weiss DG. Study on cell survival, induction of apoptosis and micronucleus formation in SCL-II cells after exposure to the auger electron emitter 99mTc. Int J Radiat Biol. 2004a;80(11–12):875–80.

    CAS  PubMed  Article  Google Scholar 

  • Kriehuber R, Riedling M, Simkó M, Weiss DG. Cytotoxicity, genotoxicity and intracellular distribution of the Auger electron emitter 65Zn in two human cell lines. Radiat Environ Biophys. 2004b;43(1):15–22.

    PubMed  Article  Google Scholar 

  • Kwon LY, Scollard DA, Reilly RM. 64Cu-labeled trastuzumab fab-PEG24-EGF radioimmunoconjugates bispecific for HER2 and EGFR: pharmacokinetics, biodistribution, and tumor imaging by PET in comparison to monospecific agents. Mol Pharm. 2017;14(2):492–501.

    CAS  PubMed  Article  Google Scholar 

  • Lai P, Cai Z, Pignol JP, Lechtman E, Mashouf S, Lu Y, Winnik MA, Jaffray DA, Reilly RM. Monte Carlo simulation of radiation transport and dose deposition from locally released gold nanoparticles labeled with 111In, 177Lu or 90Y incorporated into tissue implantable depots. Phys Med Biol. 2017;62(22):8581–99.

    CAS  PubMed  Article  Google Scholar 

  • Larionov AA. Current therapies for human epidermal growth factor receptor 2-positive metastatic breast cancer patients. Front Oncol. 2018;8:89.

    PubMed  PubMed Central  Article  Google Scholar 

  • Lee H, Hoang B, Fonge H, Reilly RM, Allen C. In vivo distribution of polymeric nanoparticles at the whole-body, tumor, and cellular levels. Pharm Res. 2010;27(11):2343–55.

    CAS  PubMed  Article  Google Scholar 

  • Lever JR, Fergason-Cantrell EA, Carmack TL, Watkinson LD, Gallazzi F. Design, synthesis and evaluation of [111In]-labeled, DOTA-conjugated tetrapeptides having high affinity and selectivity for mu opioid receptors. Nucl Med Biol. 2019;70:53–66.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Leyton JV, Hu M, Gao C, Turner PV, Dick JE, Minden M, Reilly RM. Auger electron radioimmunotherapeutic agent specific for the CD123+/CD131− phenotype of the leukemia stem cell population. J Nucl Med. 2011;52(9):1465–73.

    CAS  PubMed  Article  Google Scholar 

  • Leyton JV, Williams B, Gao C, Keating A, Minden M, Reilly RM. MicroSPECT/CT imaging of primary human AML engrafted into the bone marrow and spleen of NOD/SCID mice using 111In-DTPA-NLS-CSL360 radioimmunoconjugates recognizing the CD123+/CD131− epitope expressed by leukemia stem cells. Leuk Res. 2014;38(11):1367–73.

    CAS  PubMed  Article  Google Scholar 

  • Li L, Quang TS, Gracely EJ, Kim JH, Emrich JG, Yaeger TE, Jenrette JM, Cohen SC, Black P, Brady LW. A phase II study of anti–epidermal growth factor receptor radioimmunotherapy in the treatment of glioblastoma multiforme. J Neurosurg. 2010;113(2):192–8.

    PubMed  Article  Google Scholar 

  • Limouris GS, Chatziioannou A, Kontogeorgakos D, Mourikis D, Lyra M, Dimitriou P, Stavraka A, Gouliamos A, Vlahos L. Selective hepatic arterial infusion of in-111-DTPA-Phe1-octreotide in neuroendocrine liver metastases. Eur J Nucl Med Mol Imaging. 2008;35(10):1827–37.

    CAS  PubMed  Article  Google Scholar 

  • Lobachevsky PN, White J, Leung M, Skene C, White J, Martin RF. Plasmid breakage by 125I-labelled DNA ligands: effect of DNA-iodine atom distance on breakage efficiency. Int J Radiat Biol. 2008;84(12):991–1000.

    CAS  PubMed  Article  Google Scholar 

  • Loevinger R, Budinger TF, Watson EE. MIRD primer for absorbed dose calculations. New York: Society of Nuclear Medicine; 1988.

    Google Scholar 

  • López-Coello LI, Torres-García E, Díaz-Sánchez LE, Oros-Pantoja R, Aranda-Lara L. Differences in the S value between male and female murine model for diagnostic, therapeutic and theragnostic radionuclides. Int J Rad Appl Instrum A. 2019;146:61–5.

    Google Scholar 

  • Ma J, Kumar A, Muroya Y, Yamashita S, Sakurai T, Denisov SA, Sevilla MD, Adhikary A, Seki S, Mostafavi M. Observation of dissociative quasi-free electron attachment to nucleoside via excited anion radical in solution. Nat Commun. 2019;10(1):102.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  • Macapinlac HA, Kemeny N, Daghighian F, Finn R, Zhang J, Humm J, Squire O, Larson SM. Pilot clinical trial of 5-[125I] iodo-2′-deoxyuridine in the treatment of colorectal cancer metastatic to the liver. J Nucl Med. 1996;37(4 Suppl):25S–9S.

    CAS  PubMed  Google Scholar 

  • Mah LJ, Orlowski C, Ververis K, El-Osta AC, Karagiannis T. Utility of γH2AX as a molecular marker of DNA double-strand breaks in nuclear medicine: applications to radionuclide therapy employing auger electron-emitting isotopes. Curr Radiopharm. 2011;4(1):59–67.

    CAS  PubMed  Article  Google Scholar 

  • Makrigiorgos GM, Kassis AI, Baranowska-Kortylewicz J, McElvany KD, Welch MJ, Sastry KS, Adelstein SJ. Radiotoxicity of 5-[123I] iodo-2′-deoxyuridine in V79 cells: a comparison with 5-[125I] iodo-2′-deoxyuridine. Radiat Res. 1989;118(3):532–44.

    CAS  PubMed  Article  Google Scholar 

  • Marín A, Martín M, Liñán O, Alvarenga F, López M, Fernández L, Büchser D, Cerezo L. Bystander effects and radiotherapy. Rep Pract Oncol Radiother. 2015;20(1):12–21.

    PubMed  Article  Google Scholar 

  • Massari R, D’Elia A, Soluri A. A new high-resolution imaging system (HiRIS2) detector for preclinical SPECT imaging. Nucl Instrum Methods Phys Res A. 2019;917:25–30.

    CAS  Article  Google Scholar 

  • Mattsson S, Johansson L, Leide SS, Liniecki J, Noßke D, Riklund KÅ, Stabin M, Taylor D, Bolch W, Carlsson S, Eckerman K. Radiation dose to patients from radiopharmaceuticals: a compendium of current information related to frequently used substances. Ann ICRP. 2015;44(Suppl. 2):7–321.

    CAS  PubMed  Article  Google Scholar 

  • McGuire E. Auger and Coster Kronig Transitions. In: Crasemann B, editor. Atomic Inner-Shell Processes. I Ionization and Transition Probabilities. New York: Academic Press; 1975. p. 294.

    Google Scholar 

  • Meitner L. Über die Entstehung der β-Strahl-Spektren radioaktiver Substanzen. Z Physik. 1922;9(1):131–44.

    CAS  Article  Google Scholar 

  • Michel RB, Brechbiel MW, Mattes MJ. A comparison of 4 radionuclides conjugated to antibodies for single-cell kill. J Nucl Med. 2003;44(4):632–40.

    CAS  PubMed  Google Scholar 

  • Michel RB, Castillo ME, Andrews PM, Mattes MJ. In vitro toxicity of A-431 carcinoma cells with antibodies to epidermal growth factor receptor and epithelial glycoprotein-1 conjugated to radionuclides emitting low-energy electrons. Clin Cancer Res. 2004;10(17):5957–66.

    CAS  PubMed  Article  Google Scholar 

  • Michel RB, Rosario AV, Andrews PM, Goldenberg DM, Mattes MJ. Therapy of small subcutaneous B-lymphoma xenografts with antibodies conjugated to radionuclides emitting low-energy electrons. Clin Cancer Res. 2005;11(2):777–86.

    CAS  PubMed  Google Scholar 

  • MIRDcell, a Multicellular Dosimetry Tool (n.d.). Available at:

  • Mothersill C, Rusin A, Fernandez-Palomo C, Seymour C. History of bystander effects research 1905-present; what is in a name? Int J Radiat Biol. 2018;94(8):696–707.

    CAS  PubMed  Article  Google Scholar 

  • Müller C, Umbricht CA, Gracheva N, Tschan VJ, Pellegrini G, Bernhardt P, Zeevaart JR, Köster U, Schibli R, van der Meulen NP. Terbium-161 for PSMA-targeted radionuclide therapy of prostate cancer. Eur J Nucl Med Mol Imaging. 2019:1–2.

  • Narra VR, Harapanhalli RS, Goddu SM, Howell RW, Rao DV. Radioprotection against biological effects of internal radionuclides in vivo by S-(2-aminoethyl) isothiouronium bromide hydrobromide (AET). J Nucl Med. 1995;36(2):259–66.

    CAS  PubMed  Google Scholar 

  • Ngo Ndjock Mbong G, Lu Y, Chan C, Cai Z, Liu P, Boyle AJ, Winnik MA, Reilly RM. Trastuzumab labeled to high specific activity with 111In by site-specific conjugation to a metal-chelating polymer exhibits amplified Auger electron-mediated cytotoxicity on HER2-positive breast cancer cells. Mol Pharm. 2015;12(6):1951–60.

    CAS  PubMed  Article  Google Scholar 

  • Ocampo-García BE, Santos-Cuevas CL, Luna-Gutiérrez MA, Ignacio-Alvarez E, Pedraza-López M, Manzano-Mayoral C. 99mTc-exendin (9-39)/octreotide: biokinetics and radiation dosimetry in healthy individuals. Nucl Med Commun. 2017;38(11):912–8.

    PubMed  Article  CAS  Google Scholar 

  • Olayioye MA, Neve RM, Lane HA, Hynes NE. The ErbB signaling network: receptor heterodimerization in development and cancer. EMBO J. 2000;19(13):3159–67.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Olive PL, Banath JP. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and 125IdUrd. Int J Radiat Biol. 1993;64(4):349–58.

    CAS  PubMed  Article  Google Scholar 

  • Paillas S, Ladjohounlou R, Lozza C, Pichard A, Boudousq V, Jarlier M, Sevestre S, Le Blay M, Deshayes E, Sosabowski J, Chardes T. Localized irradiation of cell membrane by auger electrons is cytotoxic through oxidative stress-mediated nontargeted effects. Antioxid Redox Signal. 2016;25(8):467–84.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Panosa C, Fonge H, Ferrer-Batallé M, Menéndez JA, Massaguer A, De Llorens R, Reilly RM. A comparison of non-biologically active truncated EGF (EGFt) and full-length hEGF for delivery of Auger electron-emitting 111In to EGFR-positive breast cancer cells and tumor xenografts in athymic mice. Nucl Med Bio. 2015;42(12):931–8.

    CAS  Article  Google Scholar 

  • Panyutin IG, Neumann RD. Radioprobing of DNA: distribution of DNA breaks produced by decay of 125I incorporated into a triplex-forming oligonucleotide correlates with geometry of the triplex. Nucleic Acids Res. 1997;25(4):883–7.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Pedraza-López M, Ferro-Flores G, Mendiola-Cruz MT, Morales-Ramirez P. Assessment of radiation-induced DNA damage caused by the incorporation of 99mTc-radiopharmaceuticals in murine lymphocytes using single cell gel electrophoresis. Mutat Res Genet Toxicol Environ Mutagen. 2000;465(1–2):139–44.

    Article  Google Scholar 

  • Piron B, Paillas S, Boudousq V, Pèlegrin A, Bascoul-Mollevi C, Chouin N, Navarro-Teulon I, Pouget JP. DNA damage-centered signaling pathways are effectively activated during low dose-rate Auger radioimmunotherapy. Nucl Med Bio. 2014;41:e75–83.

    CAS  Article  Google Scholar 

  • Pouget JP, Georgakilas AG, Ravanat JL. Targeted and off-target (bystander and abscopal) effects of radiation therapy: redox mechanisms and risk/benefit analysis. Antioxid Redox Signal. 2018;29(15):1447–87.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Pouget JP, Santoro L, Raymond L, Chouin N, Bardiès M, Bascoul-Mollevi C, Huguet H, Azria D, Kotzki PO, Pèlegrin M, Vivès E. Cell membrane is a more sensitive target than cytoplasm to dense ionization produced by auger electrons. Radiat Res. 2008;170(2):192–200.

    CAS  PubMed  Article  Google Scholar 

  • Psyrri A, Kwong M, DiStasio S, Lekakis L, Kassar M, Sasaki C, Wilson LD, Haffty BG, Son YH, Ross DA, Weinberger PM. Cisplatin, fluorouracil, and leucovorin induction chemotherapy followed by concurrent cisplatin chemoradiotherapy for organ preservation and cure in patients with advanced head and neck cancer: long-term follow-up. J Clin Oncol. 2004;22(15):3061–9.

    CAS  PubMed  Article  Google Scholar 

  • Rao D, Howell R, Narra V, Govelitz G, Sastry KR. In-vivo radiotoxicity of DNA-incorporated 125I compared with that of densely ionising alpha-particles. Lancet. 1989;334(8664):650–3.

    Article  Google Scholar 

  • Rao DV, Narra VR, Howell RW, Sastry KS. Biological consequence of nuclear versus cytoplasmic decays of125 I: Cysteamine as a radioprotector against auger cascades in vivo. Radiat Res. 1990;124(2):188–93.

    CAS  PubMed  Article  Google Scholar 

  • Razumienko EJ, Chen JC, Cai Z, Chan C, Reilly RM. Dual-receptor–targeted radioimmunotherapy of human breast cancer xenografts in athymic mice coexpressing HER2 and EGFR using 177Lu-or 111In-labeled bispecific radioimmunoconjugates. J Nucl Med. 2016;57(3):444–52.

    CAS  PubMed  Article  Google Scholar 

  • Razumienko EJ, Dryden L, Scollard D, Reilly RM. MicroSPECT/CT imaging of co-expressed HER2 and EGFR on subcutaneous human tumor xenografts in athymic mice using 111In-labeled bispecific radioimmunoconjugates. Breast Cancer Res Treat. 2013;138(3):709–18.

    CAS  PubMed  Article  Google Scholar 

  • Rebischung C, Hoffmann D, Stefani L, Desruet MD, Wang K, Adelstein SJ, Artignan X, Vincent F, Gauchez AS, Zhang H, Fagret D. First human treatment of resistant neoplastic meningitis by intrathecal administration of MTX plus 125IUdR. Int J Radiat Biol. 2008;84(12):1123–9.

    CAS  PubMed  Article  Google Scholar 

  • Regulla D, Schmid E, Friedland W, Panzer W, Heinzmann U, Harder D. Enhanced values of the RBE and H ratio for cytogenetic effects induced by secondary electrons from an X-irradiated gold surface. Radiat Res. 2002;158(4):505–15.

    CAS  PubMed  Article  Google Scholar 

  • Reilly RM. The radiopharmaceutical science of monoclonal antibodies and peptides for imaging and targeted in situ radiotherapy of malignancies. In: Gad SC, editor. Handbook of pharmaceutical biotechnology. Toronto: John Wiley & Sons; 2007. p. 987–1053.

    Google Scholar 

  • Reilly RM, Kassis A. Targeted Auger electron radiotherapy of malignancies. In: Reilly RM, editor. Monoclonal antibody and peptide-targeted radiotherapy of cancer. Hoboken: John Wiley & Sons; 2010. p. 289–348.

    Chapter  Google Scholar 

  • Reilly RM, Kiarash R, Cameron RG, Porlier N, Sandhu J, Hill RP, Vallis K, Hendler A, Gariépy J. 111In-labeled EGF is selectively radiotoxic to human breast cancer cells overexpressing EGFR. J Nucl Med. 2000;41(3):429–38.

    CAS  PubMed  Google Scholar 

  • Reilly RM, Scollard DA, Wang J, Mondal H, Chen P, Henderson LA, Bowen BM, Vallis KA. A kit formulated under good manufacturing practices for labeling human epidermal growth factor with 111In for radiotherapeutic applications. J Nucl Med. 2004;45(4):701–8.

    CAS  PubMed  Google Scholar 

  • Reske SN, Deisenhofer S, Glatting G, Zlatopolskiy BD, Morgenroth A, Vogg AT, Buck AK, Friesen C. 123I-ITdU–mediated nanoirradiation of DNA efficiently induces cell kill in HL60 leukemia cells and in doxorubicin-, β-, or γ-radiation–resistant cell lines. J Nucl Med. 2007;48(6):1000–7.

    CAS  PubMed  Article  Google Scholar 

  • Roeske JC, Aydogan B, Bardies M, Humm JL. Small-scale dosimetry: challenges and future directions. Semin Nucl Med. 2008;38:367–83.

    CAS  PubMed  Article  Google Scholar 

  • Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem. 1998;273(10):5858–68.

    CAS  PubMed  Article  Google Scholar 

  • Sahu SK, Wen PY, Foulon CF, Nagel JS. Intrathecal 5-(125I) Iodo-2′-deoxyuridine in a rat model of leptomeningeal metastases. J Nucl Med. 1997;38(3):386.

    CAS  PubMed  Google Scholar 

  • Salem R, Lewandowski RJ, Atassi B, Gordon SC, Gates VL, Barakat O, Sergie Z, Wong CY, Thurston KG. Treatment of unresectable hepatocellular carcinoma with use of 90Y microspheres (TheraSphere): safety, tumor response, and survival. J Vasc Interv Radiol. 2005;16(12):1627–39.

    PubMed  Article  Google Scholar 

  • Salomon DS, Brandt R, Ciardiello F, Normanno N. Epidermal growth factor-related peptides and their receptors in human malignancies. Crit Rev Oncol Hematol. 1995;19(3):183–232.

    CAS  PubMed  Article  Google Scholar 

  • Santoro L, Boutaleb S, Garambois V, Bascoul-Mollevi C, Boudousq V, Kotzki PO, Pèlegrin M, Navarro-Teulon I, Pèlegrin A, Pouget JP. Noninternalizing monoclonal antibodies are suitable candidates for 125I radioimmunotherapy of small-volume peritoneal carcinomatosis. J Nucl Med. 2009;50(12):2033–41.

    PubMed  Article  Google Scholar 

  • Sarnelli A, Guerriero F, Botta F, Ferrari M, Strigari L, Bodei L, D'Errico V, Grassi E, Fioroni F, Paganelli G, Orecchia R. Therapeutic schemes in 177Lu and 90Y-PRRT: radiobiological considerations. Q J Nucl Med Mol Imaging. 2017;61(2):216–31.

    PubMed  Article  Google Scholar 

  • Schneider DO, Whitmore GF. Comparative effects of neutrons and X-rays on mammalian cells. Radiat Res. 1963;18(3):286–306.

    CAS  PubMed  Article  Google Scholar 

  • Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM. Quantitative detection of 125IdU-induced DNA double-strand breaks with γ-H2AX antibody. Radiat Res. 2002;158(4):486–92.

    CAS  PubMed  Article  Google Scholar 

  • Shrivastava S, Mahantshetty U, Engineer R, Chopra S, Hawaldar R, Hande V, Kerkar RA, Maheshwari A, Shylasree TS, Ghosh J, Bajpai J. Cisplatin chemoradiotherapy vs radiotherapy in FIGO stage IIIB squamous cell carcinoma of the uterine cervix: a randomized clinical trial. JAMA Oncol. 2018;4(4):506–13.

    PubMed  PubMed Central  Article  Google Scholar 

  • Siegel JA, Thomas SR, Stubbs JB, Stabin MG, Hays MT, Koral KF, Robertson JS, Howell RW, Wessels BW, Fisher DR, Weber DA. MIRD pamphlet no. 16: techniques for quantitative radiopharmaceutical biodistribution data acquisition and analysis for use in human radiation dose estimates. J Nucl Med. 1999;40(2):37S–61S.

    CAS  PubMed  Google Scholar 

  • Silver DA, Pellicer I, Fair WR, Heston WD, Cordon-Cardo C. Prostate-specific membrane antigen expression in normal and malignant human tissues. Clin Cancer Res. 1997;3(1):81–5.

    CAS  PubMed  Google Scholar 

  • Slastnikova TA, Koumarianou E, Rosenkranz AA, Vaidyanathan G, Lupanova TN, Sobolev AS, Zalutsky MR. Modular nanotransporters: a versatile approach for enhancing nuclear delivery and cytotoxicity of Auger electron-emitting 125I. EJNMMI Res. 2012;2(1):59.

    PubMed  PubMed Central  Article  CAS  Google Scholar 

  • Sobolev AS. Modular nanotransporters for nuclear-targeted delivery of auger electron emitters. Front Pharmacol. 2018;9.

  • Song L, Able S, Johnson E, Vallis KA. Accumulation of 111In-labelled EGF-au-PEG nanoparticles in EGFR-positive tumours is enhanced by coadministration of targeting ligand. Nanotheranostics. 2017;1(3):232–43.

    PubMed  PubMed Central  Article  Google Scholar 

  • Song L, Falzone N, Vallis KA. EGF-coated gold nanoparticles provide an efficient nano-scale delivery system for the molecular radiotherapy of EGFR-positive cancer. Int J Radiat Biol. 2016;92(11):716–23.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Stabin M. Nuclear medicine dosimetry. Phys Med Biol. 2006;51(13):R187–202.

    CAS  PubMed  Article  Google Scholar 

  • Stabin MG, Siegel JA. RADAR dose estimate report: a compendium of radiopharmaceutical dose estimates based on OLINDA/EXM version 2.0. J Nucl Med. 2018;59(1):154–60.

    PubMed  Article  Google Scholar 

  • Stabin MG, Siegel JA, Sparks RB, Eckerman KF, Breitz HB. Contribution to red marrow absorbed dose from total body activity: a correction to the MIRD method. J Nucl Med. 2001;42(3):492–8.

    CAS  PubMed  Google Scholar 

  • Stabin MG, Sparks RB, Crowe E. OLINDA/EXM: the second-generation personal computer software for internal dose assessment in nuclear medicine. J Nucl Med. 2005;46(6):1023–7.

    PubMed  Google Scholar 

  • Strigari L, Konijnenberg M, Chiesa C, Bardies M, Du Y, Gleisner KS, Lassmann M, Flux G. The evidence base for the use of internal dosimetry in the clinical practice of molecular radiotherapy. Eur J Nucl Med Mol Imaging. 2014;41(10):1976–88.

    CAS  PubMed  Article  Google Scholar 

  • Tajik-Mansoury MA, Rajabi H, Mozdarani H. A comparison between track-structure, condensed-history Monte Carlo simulations and MIRD cellular S-values. Phys Med Biol. 2017;62(5):N90–N106.

    CAS  PubMed  Article  Google Scholar 

  • Thierens HM, Monsieurs MA, Brans B, Van Driessche T, Christiaens I, Dierckx RA. Dosimetry from organ to cellular dimensions. Comput Med Imaging Graph. 2001;25(2):187–93.

    CAS  PubMed  Article  Google Scholar 

  • Urashima T, Nagasawa H, Wang K, Adelstein SJ, Little JB, Kassis AI. Induction of apoptosis in human tumor cells after exposure to Auger electrons: comparison with γ-ray exposure. Nucl Med Biol. 2006;33(8):1055–63.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Uusijärvi H, Bernhardt P, Rösch F, Maecke HR, Forssell-Aronsson E. Electron-and positron-emitting radiolanthanides for therapy: aspects of dosimetry and production. J Nucl Med. 2006;47(5):807–14.

    PubMed  Google Scholar 

  • Valkema R, De Jong M, Bakker WH, Breeman WA, Kooij PP, Lugtenburg PJ, De Jong FH, Christiansen A, Kam BL, De Herder WW, Stridsberg M. Phase I study of peptide receptor radionuclide therapy with [111In-DTPA0] octreotide: the Rotterdam experience. Semin Nucl Med. 2002;32:110.

    PubMed  Article  Google Scholar 

  • Vallabhajosula S, Goldsmith SJ, Hamacher KA, Kostakoglu L, Konishi S, Milowski MI, Nanus DM, Bander NH. Prediction of myelotoxicity based on bone marrow radiation-absorbed dose: radioimmunotherapy studies using 90Y-and 177Lu-labeled J591 antibodies specific for prostate-specific membrane antigen. J Nucl Med. 2005;46(5):850–8.

    CAS  PubMed  Google Scholar 

  • Vallis KA, Reilly RM, Scollard D, Merante P, Brade A, Velauthapillai S, Caldwell C, Chan I, Freeman M, Lockwood G, Miller NA. Phase I trial to evaluate the tumor and normal tissue uptake, radiation dosimetry and safety of 111In-DTPA-human epidermal growth factor in patients with metastatic EGFR-positive breast cancer. Am J Nucl Med Mol Imaging. 2014;4(2):181–92.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Vaziri B, Wu H, Dhawan AP, Du P, Howell RW, Bolch WE, Brill AB, Dewaraja YK, Dunphy MP, Fisher DR, Meredith RF. MIRD pamphlet no. 25: MIRDcell V2. 0 software tool for dosimetric analysis of biologic response of multicellular populations. J Nucl Med. 2014;55(9):1557–64.

    PubMed  Article  Google Scholar 

  • Wang YN, Hung MC. Nuclear functions and subcellular trafficking mechanisms of the epidermal growth factor receptor family. Cell Biosci. 2012;2(1):13.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Wilson CT. Investigations on X-rays and α-rays by the cloud method. Nature. 1923;112:26–7.

    Article  Google Scholar 

  • Woo DV, Li D, Mattis JA, Steplewski Z. Selective chromosomal damage and cytotoxicity of 125I-labeled monoclonal antibody 17-1a in human cancer cells. Cancer Res. 1989;49(11):2952–8.

    CAS  PubMed  Google Scholar 

  • Xue LY, Butler NJ, Makrigiorgos GM, Adelstein SJ, Kassis AI. Bystander effect produced by radiolabeled tumor cells in vivo. Proc Natl Acad Sci U S A. 2002;99(21):13765–70.

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  • Yasui LS, Chen K, Wang K, Jones TP, Caldwell J, Guse D, Kassis AI. Using Hoechst 33342 to target radioactivity to the cell nucleus. Radiat Res. 2007;167(2):167–75.

    CAS  PubMed  Article  Google Scholar 

  • Yasui LS, Hughes A, DeSombre ER. Relative biological effectiveness of accumulated 125IdU and 125I-estrogen decays in estrogen receptor-expressing MCF-7 human breast cancer cells. Radiat Res. 2001;155(2):328–34.

    CAS  PubMed  Article  Google Scholar 

  • Zatloukal P, Petruzelka L, Zemanova M, Havel L, Janku F, Judas L, Kubik A, Krepela E, Fiala P, Pecen L. Concurrent versus sequential chemoradiotherapy with cisplatin and vinorelbine in locally advanced non-small cell lung cancer: a randomized study. Lung Cancer. 2004;46(1):87–98.

    PubMed  Article  Google Scholar 

  • Zereshkian A, Leyton JV, Cai Z, Bergstrom D, Weinfeld M, Reilly RM. The human polynucleotide kinase/phosphatase (hPNKP) inhibitor A12B4C3 radiosensitizes human myeloid leukemia cells to Auger electron-emitting anti-CD123 111In-NLS-7G3 radioimmunoconjugates. Nucl Med Biol. 2014;41(5):377–83.

    CAS  PubMed  Article  Google Scholar 

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The authors acknowledge research grant funding from the Canadian Breast Cancer Foundation, Canadian Cancer Society, and the Canadian Institutes of Health Research. AK received a scholarship from the Centre for Pharmaceutical Oncology at the University of Toronto and a Queen Elizabeth II Graduate Scholarship in Science and Technology (QEII-GSST). VF was supported by the Polymer Nanoparticles for Drug Delivery (POND) CREATE training program sponsored by the Natural Sciences and Engineering Research Council of Canada and the MDS Nordion Graduate Scholarship in Radiopharmaceutical Sciences (OTSS) award. The publication of this article was supported by funds of the European Association of Nuclear Medicine (EANM).

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AK and VF contributed equally to this article. RMR conceived of the article design and edited the final manuscript. AK, VF and ZC researched the literature and drafted the manuscript. All authors reviewed and approved the final manuscript.

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Correspondence to Raymond M. Reilly.

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Ku, A., Facca, V.J., Cai, Z. et al. Auger electrons for cancer therapy – a review. EJNMMI radiopharm. chem. 4, 27 (2019).

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  • Auger electrons
  • 111In
  • Monoclonal antibodies
  • Nanoparticles
  • Peptides
  • Dosimetry
  • Radiolabelling
  • Cancer treatment
  • Preclinical studies
  • Clinical studies