Table 3 The final HPLC conditions proposed
From: An alternative methodology for the determination of the radiochemical purity of 11C-methionine
Column | Macherey-Nagel - EC250/4.6 NUCLEODUR 100–5 C18ec (MN) | |
|---|---|---|
Flow | 1.0 mL/min. | |
Run time | 16 min. | |
Solvent A | KH2PO4 1,4 g/L | |
Solvent B | CH3CN | |
Gradient | % A | % B |
0 to 6 min | 99 to 98 | 1 to 2 |
6 to 8 min | 98 to 20 | 2 to 80 |
8 to 16 min | 20 | 80 |